Polymorphism study of growth differentiation factor 9B (GDF9B) gene and its association with reproductive traits in sheep

Shabir, Mir; Ganai, T. A. S.; Misra, S. S.; Shah, Ruksana; Ahmad, Tavsief

doi:10.1016/j.gene.2012.12.018

Cited by 8 publications

(4 citation statements)

References 18 publications

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“…A large number of studies have been performed on various sheep breeds to identify the candidate genes that are involved in controlling the ovulation rate and prolificacy (Kumari et al, 2016). Three genes of the ovary-derived transforming growth factor β (TGFβ) superfamily (Shabir M et al, 2013), including bone morphogenetic protein receptor-type 1B (BMPR1B/FecB), also called booroola gene (Chu et al, 2007); growth differentiation factor 9 (GDF9/FecG) (Feng et al, 2011); and bone morphogenetic protein 15 (BMP15/FecX) (Wang et al, 2011) have been reported to be the major candidate genes for prolificacy in sheep (Polley et al, 2009b).…”

Section: Introductionmentioning

confidence: 99%

PCR-RFLP-based identification of polymorphisms in BMPR1B, GDF9 and BMP15 genes associated with litter size in Beetal and Teddy goats

Islam¹,

Basheer²,

Javed³

et al. 2019

SA J. An. Sci.

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The genes BMPR1B, GDF9 and BMP15 are oocyte-derived members of the transforming growth factor-β superfamily, which is essential for follicular growth and ovulation. The aim of the current study was to determine the incidence of reported mutations in exon 3 of BMPR1B gene, exon 1 of GDF9 gene, and exon 2 of BMP15 gene and their association with litter size in three parities of Beetal and Teddy goats. Based on the known mutations in these genes, PCR primers were designed to find out the polymorphism through the PCR-RFLP technique in 120 randomly selected animals of the two breeds, which vary in their prolificacy. The current results show that there were significant differences in litter size [1.708 ± 0.060 in Beetal (B) and 2.167 ± 0.056 in Teddy (T) goats] in both breeds. The results of breed x parity interaction showed significant differences in litter size in first [1.158 ± 0.061 (B); 1.861 ± 0.057 (T)], third [2.062 ± 0.078 (B); 2.583 ± 0.073 (T)] and average parity [708 ± 0.060 (B); 2.167 ± 0.056 (T)] in both breeds. All three loci in both breeds were found to be polymorphic and the results of breed x parity x genotype analysis showed that genotypes of all the three genes were significantly associated with litter size in all parities in both breeds. For the BMPR1B gene, heterozygous (CT) animals of Beetal and Teddy goats had the largest litter size in all three parities and the average (parity) litter size, showing the overdominance of the heterozygotes. Similarly, for GDF9 gene, heterozygous animals (AG) had the largest litter size in both breeds. For BMP15 gene homozygous carrier animals (CC) of both breeds had the highest number of kids, followed by the heterozygous (AC) genotypes. The current results show the importance of BMPR1B, GDF9 and BMP15 as the major genes that influence prolificacy in both Beetal and Teddy goats. It is suggested that these polymorphisms can be used as molecular markers to select the prolific animals in these breeds.

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Section: Introductionmentioning

confidence: 99%

PCR-RFLP-based identification of polymorphisms in BMPR1B, GDF9 and BMP15 genes associated with litter size in Beetal and Teddy goats

Islam¹,

Basheer²,

Javed³

et al. 2019

SA J. An. Sci.

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“…Li L et al (2009) found two SNPs in rabbit BMP15, and the mean litter size of BB genotypes was higher than that of AA and AN genotype in angora rabbit flock (P<0.05) [ 34 ]. Shabir M, et al (2013) found that SNP "C" of mutations in exon-2 of the designated genotype AC was observed to produce a significant effect on litter size with average litter size going up by 0.63, as compared with the nearest genotype AB with the litter size of 1.29±0.05 [ 35 ]. In sheep, eight genetic mutations with major effects on ovulation rate and litter size have been identified in BMP15 [ 22 – 27 ].…”

Section: Discussionmentioning

confidence: 99%

Association between BMP15 Gene Polymorphism and Reproduction Traits and Its Tissues Expression Characteristics in Chicken

et al. 2015

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BMP15 (Bone morphogenetic protein 15) is an oocyte-secreted growth factor required for ovarian follicle development and ovulation in mammals, but its effects on reproduction in chickens are unclear. In this study, the association between BMP15 polymorphisms and reproduction traits were analyzed, and its expression characteristics in different tissues were explored in LaiWu Black chickens. Three single nucleotide polymorphisms (SNPs) were identified in four hundred LaiWu Black chickens. One SNP (NC_006091.3:g.1773T>C) located in exon 2 which was significantly associated with egg weight at first egg (EWFE) (P = 0.0389), was novel. Diplotypes based on the three SNPs were found to be significantly associated with egg weight at age of 43W (EW43) (P = 0.0058). The chickens with H3H3 diplotype had their first egg 0.57 days later than chickens with H5H5 diplotype and 1.21 days-3.96 days earlier than the other five diplotype chickens. The egg production at age of 43W (E43), egg production at age of 46W (E46) and egg production at age of 48W (E48) for chickens with H3H3 diplotype were the highest among all the chickens, and the E48 of chickens with H3H3 diplotype had 11.83 eggs higher than chickens with H1H5 diplotype. RT-qPCR results showed that the expression level of BMP15 gene in ovarian follicle was in the order of 4 mm>6 mm -8 mm> 15 mm -19 mm> 23 mm -29 mm > 33 mm -34 mm in diameter. The mRNA level in follicles of 4 mm and 6–8 mm in diameter were significantly higher than that in the other follicles (P<0.01). In the same week, the highest mRNA level was found in the ovary, and it was significantly different from that found in the liver and oviduct (P<0.01). Our results indicate that BMP15 plays a vital role in the development of ovary and follicles, especially in the development of primary follicles. H3H3 may be an potential advantageous molecular marker for improving reproduction traits in chickens.

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“…The BMP15 gene is located on ovine chromosome X: 50970938‐50977454, with 2 exons, is annotated with seven domains and features and is associated with 10 variations (http://ensembl.org). There are several point mutations in BMP15 gene (Table ), identified in different sheep breeds (Bodin et al., ; Demars et al., ; Galloway et al., ; Hanrahan et al., ; Monteagudo, Ponz, Tejedor, Lavina, & Sierra, ; Shabir, Ganai, Misra, Shah, & Ahmad, ; Zamani, Nadri et al, ).…”

Section: Bone Morphogenetic Protein 15mentioning

confidence: 99%

A review on prolificacy genes in sheep

Abdoli

Zamani

Mirhoseini

et al. 2016

Reprod Domestic Animals

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Ovulation rate and litter size are important reproduction traits in sheep and are of high economic value. Reproduction traits typically have low to medium heritabilities and do not exhibit a noticeable response to phenotypic selection. Therefore, inclusion of genetic information of the genes associated with reproductive ability could efficiently enhance the selection response. The most important major genes affecting prolificacy and their genetic diversities in different sheep breeds were reviewed. Different causative mutations with major effects on reproductive traits including ovulation rate and litter size have been found in various sheep breeds around the world. A general overview of the studies on main prolificacy genes showed that some alleles may express different phenotypic effects in different breeds, and thus, further studies on epistatic effects are necessary for more understanding of genetic control of reproductivity in sheep. Regarding the polygenic control of fertility traits, application of new high-throughput technologies to find new variants is essential for future studies. Moreover, genomewide association studies and genomic best linear unbiased predictions of breeding values are likely to be effective tools for genetic improvement of sheep reproductive performance traits.

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Polymorphism study of growth differentiation factor 9B (GDF9B) gene and its association with reproductive traits in sheep

Cited by 8 publications

References 18 publications

PCR-RFLP-based identification of polymorphisms in <i>BMPR1B</i>, <i>GDF9</i> and <i>BMP15</i> genes associated with litter size in Beetal and Teddy goats

PCR-RFLP-based identification of polymorphisms in <i>BMPR1B</i>, <i>GDF9</i> and <i>BMP15</i> genes associated with litter size in Beetal and Teddy goats

Association between BMP15 Gene Polymorphism and Reproduction Traits and Its Tissues Expression Characteristics in Chicken

A review on prolificacy genes in sheep

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