Polymorphisms in gyrA and gyrB Genes among Mycobacterium avium subsp. paratuberculosis Type I, II, and III Isolates

Abstract

The analysis of the gyrA and gyrB genes of a panel of Mycobacterium avium subsp. paratuberculosis isolates from types I, II, and III detected type-specific single nucleotide polymorphisms. Based on these results, we developed a PCR and restriction enzyme analysis to discriminate type I and III isolates. The application of this technique would be the unique strategy to characterize these strains when there is not enough bacterial growth to perform pulsed-field gel electrophoresis and IS900 restriction fragment length polymorphism.

Multiplexed Typing of Mycobacterium avium subsp. paratuberculosis Types I, II, and III by Luminex xMAP Suspension Array

“…In particular, Castellanos et al identified SNPs in the gyrase B (gyrB) gene that allow discriminating all three types of M. avium subsp. paratuberculosis (3). The authors also developed a PCR restriction enzyme analysis (PCR-REA) of gyrB locus 1626 that is capable of differentiating types I and II from type III.…”

Viable Mycobacterium avium ssp. paratuberculosis isolated from calf milk replacer

“…The consensus sequence of each sample was then extracted from CLCBio software and used to build a phylogenetic tree using HarvestTools (Treangen et al, 2014). Finally, to distinguish MAP genotypes (type I vs. II or III), hsp65, gyrA, and gyrB PCR followed by enzyme digestion were performed as detailed previously by Castellanos et al (2007) and Ghosh et al (2012).…”

Trends and advances in the diagnosis and control of paratuberculosis in domestic livestock

“…Single nucleotide polymorphism (SNP) in MAP IS900 elements has been exploited to develop PCR-REA (restriction enzyme analysis) for genotyping of MAP (animal species-specific MAP), which has enabled to track the sources and the pattern of transmission for improving pTB control programmes Castellanos et al 2007Castellanos et al , 2010.…”