Polyphenolic Compounds from Lespedeza Bicolor Root Bark Inhibit Progression of Human Prostate Cancer Cells via Induction of Apoptosis and Cell Cycle Arrest

Abstract

From a root bark of Lespedeza bicolor Turch we isolated two new (7 and 8) and six previously known compounds (1-6) belonging to the group of prenylated polyphenols. Their structures were elucidated using mass spectrometry, nuclear magnetic resonance and circular dichroism spectroscopy. These natural compounds selectively inhibited human drug-resistant prostate cancer in vitro. Prenylated pterocarpans 1-3 prevented the cell cycle progression of human cancer cells in S-phase. This was accompanied by a reduced expression of mRNA corresponding to several human cyclin-dependent kinases (CDKs). In contrast, compounds 4-8 induced a G1-phase cell cycle arrest without any pronounced effect on CDKs mRNA expression. Interestingly, a non-substituted hydroxy group at C-8 of ring D of the pterocarpan skeleton of compounds 1-3 seems to be important for the CDKs inhibitory activity.

Estrogenic Plants: to Prevent Neurodegeneration and Memory Loss and Other Symptoms in Women After Menopause

“…Catechin, genistein daidzein, luteolin, naringenin, and rutin were identified in the powdered extract of L. bicolor . The root extracts contain polyphenolic compounds that delay prostate cancer progression ( Dyshlovoy et al, 2020 ). Also, the effects of L. bicolor on cognitive function were investigated in a mice model of AD using a cerebral injection of Aβ.…”

Antileishmanial potential of immunomodulator gallic acid against experimental murine visceral leishmaniasis

“…Polyphenolic compounds isolated from Lespedeza bicolour root bark extract induced sub-G1 cell cycle arrest in human drug-resistant prostate cancer cell line PC-3. 44 Cells infected with pathogens generate reactive oxygen species (ROS) such as peroxides, hydroxyl radicals, and superoxides to fight the infection. Likewise, ROS is also produced in retaliation to certain medications; on a similar basis, antiprotozoal drugs execute parasites in infected cells.…”

Yerba Mate Modulates Tumor Cells Functions Involved in Metastasis in Breast Cancer Models

“…There is consensus that polyphenols act as anti-cancer agents by reducing cell growth, arresting cell cycle and inducing apoptosis ( Rajamani 2018 ; Chaves et al, 2020 ; Dyshlovoy et al, 2020 ). In a previous work, our group showed that YMe, which contains a complex mixture of phytochemicals, reduces cell growth and induces apoptosis due to an activation of the intrinsic pathway in a colorectal cancer model.…”