Polyploid response of <i>Artemisia annua</i> L. to colchicine treatment

Yunus, Ahmad; Parjanto,; Samanhudi,; Hikam, Maharani Putri; Widyastuti, Yuli

doi:10.1088/1755-1315/142/1/012020

Cited by 7 publications

(6 citation statements)

References 10 publications

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“…The result is that colchicine plays a role in doubling the number of chromosomes from diploid to polyploid which has a different effect on each plant. In accordance with another research which states that ploidy in plants will have a different effect according to the gene reaction and physiological reaction of the plant [8].…”

Section: Resultssupporting

confidence: 90%

Morphological Characteristics of Phalaenopsis spp. by Colchicine Application Based on Qualitative

Samanhudi¹,

Hartati²,

Cahyono³

et al. 2023

Advances in Biological Sciences Research

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Indonesia has very diverse biodiversity, such as Phalaenopsis. Phalaenopsis have the potential to be cultivated in plant breeding as parents for new hybrids. One of the attempts to improve the potential of Phalaenopsis spp. is polyploidization using colchicine. Polyploidization using colchicine can change morphological characteristics of Phalaenopsis spp. Into the desired character. This study aimed to examine the morphological characteristics of Phalaenopsis spp. Resulted from polyploidy induction by using 1000 ppm colchicine. The research was conducted at screenhouse of the Faculty of Agriculture, Sebelas Maret University, Surakarta in December 2021-March 2022. Materials used in this research were Doritaenopsis Shu Long Purple Queen x Ox King '3534', Phalaenopsis Chain Xen Moment, and Phalaenopsis Shu Long 'TS2904' which had been treated with 1000 ppm colchicine on the flower buds. Morphological observations based on the Orchid Ornamental Plant Characterization Guidebook. Observed variables consisted of 15 characters, including the characterization of leaves and flowers. Morphological diversities were was analyzed descriptively based on direct observation and documentation of orchid flower. Results showed that the treatment of 1000 ppm colchicine on orchid flower buds could change the shape of dorsal sepal tip and lateral sepal tip.

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Section: Resultssupporting

confidence: 90%

Morphological Characteristics of Phalaenopsis spp. by Colchicine Application Based on Qualitative

Samanhudi¹,

Hartati²,

Cahyono³

et al. 2023

Advances in Biological Sciences Research

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“…A decrease in stomatal density may be caused by inappropriate colchicine concentration. Other evidence also showed that polyploid plants had a lower stomatal density than diploid plants because of enlarged size of stomata per leaf area (Yulianti et al, 2015;Yunus et al, 2018).…”

Section: Resultsmentioning

confidence: 99%

Polyploidy induction by colchicine treatment in garlic (Allium sativum L.), and effects of polyploidy on some traits

Pillai¹,

Ahmed²,

Ambika³

et al. 2021

Int. J. Curr. Res. Biosci. Plant Biol.

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The garlic plant (Allium sativum L) is a suitable indicator plant for the determination of potential genotoxic agents in the samples taken from the environment. Polyploidy has the utmost importance in horticulture for the development of new varieties with desirable morphological traits referring to plant size and vigor, leaf thickness, larger flowers with thicker petals, intense color of leaves and flowers, long lasting flowers, compactness, dwarfness and restored fertility. Polyploidy may occur naturally due to the formation of unreduced gametes or can be artificially induced by doubling the number of chromosomes in somatic cells. Usually, natural polyploid plants are unavailable, so polyploidy is induced synthetically with the help of mitotic inhibitors. Colchicine is a widely used mitotic inhibitor for the induction of polyploidy in plants during their cell division by inhibiting the chromosome segregation. The aim of this study was to determine the effect of various concentrations and duration of colchicine treatment on root length, number of roots and stomatal densities Allium sativum L.

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“…The reduction in total chlorophyll content of the A. cina polyploid mutant in this study is not in line with the results of studies reported by several previous researchers. Polyploid induction is reported to increase chlorophyll content in some plants including Acacia mearnsii (Mathura et al 2006), Pogostemon cablin (Wu & Li, 2013), Stevia reboudiana (Zhang et al 2018) and Artemisia annua (Yunus et al 2018). However, Manzoor et al (2018 reported that colchicine-induced polyploid in Gladiolus grandiflorus was decreased the chlorophyll content, this result supports the result of this study.…”

Section: Resultsmentioning

confidence: 99%

Effect of Induced Polyploidy on Plant Growth, Chlorophyll and Flavonoid Content of Artemisia cina

2020

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Artemisia cina is one of a member of genus Artemisia that has potential as a medicinal plant. However, the levels of Artemisia medicinal bioactive compounds are very low. Polyploidization is an alternative method that can enhance of growth and secondary metabolite productions of plants. The aims of this research were to determine the effect of polyploid induction using colchicine and plant growth regulator toward plant growth, the chlorophyll, kaemferol and quercetin contents of A. cina. Four different A. cina used in this research consisted of two diploid genotypes (TWN and KJT) and two polyploid genotypes (J and M). Induction of mutant polyploid was conducted using colchicine and combination of plant growth regulator benziladenyl (BA) and 2,4-dichlorophenoxyacetic acid (2,4-D). The measured plant growth parameters were dry weight, leaf area, and plant height. The chlorophyll content of leaves was determined spectrophotometrically, and flavonoid content determined using HPLC. The result showed that the polyploid genotype (M) significantly decreased leaf and root growth compared to the KJT and TWN. In A. cina plants, polyploidization using colchicine is more effective in increasing the biomass than using combination plant growth regulator BA and 2,4-D. The flavonoid content of KJT was the lowest, and significantly different compared to the other plants. This study provides new information about the effect of polyploid on growth and flavonoid content in A. cina. This can be useful information to develop A. cina to become a medicinal plant.

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Polyploid response of Artemisia annua L. to colchicine treatment

Cited by 7 publications

References 10 publications

Morphological Characteristics of Phalaenopsis spp. by Colchicine Application Based on Qualitative

Morphological Characteristics of Phalaenopsis spp. by Colchicine Application Based on Qualitative

Polyploidy induction by colchicine treatment in garlic (Allium sativum L.), and effects of polyploidy on some traits

Effect of Induced Polyploidy on Plant Growth, Chlorophyll and Flavonoid Content of Artemisia cina

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