Polyploidy and microbiome associations mediate similar responses to pathogens in Arabidopsis

Mehlferber, Elijah C.; Song, Michael; Peláez, Julianne; Jaenisch, Johan; Coate, Jeremy E.; Koskella, Britt; Rothfels, Carl J.

doi:10.1016/j.cub.2022.05.015

Cited by 17 publications

(12 citation statements)

References 71 publications

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“…Similarly, synthetic tetraploids from two genotypes of A. thaliana were exposed to a single inoculum source and found to host a greater abundance, but similar diversity, of bacterial taxa in the rhizosphere compared to their diploid ancestors (Ponsford et al, 2022). However, another experiment using seven synthetic tetraploid genotypes of A. thaliana found that polyploidy did not restructure the microbiome when exposed to a synthetic community of 16 bacterial taxa but the polyploids were more tolerant to pathogenic attack than their diploid progenitors (Mehlferber et al, 2022). However, they also found that diploids inoculated with the synthetic microbiome performed better than the uninoculated diploids when attacked by pathogens (Mehlferber et al, 2022), which may imply some differential recruitment of the microbiome in polyploids versus diploids.…”

Section: Introductionmentioning

confidence: 99%

“…However, another experiment using seven synthetic tetraploid genotypes of A. thaliana found that polyploidy did not restructure the microbiome when exposed to a synthetic community of 16 bacterial taxa but the polyploids were more tolerant to pathogenic attack than their diploid progenitors (Mehlferber et al, 2022). However, they also found that diploids inoculated with the synthetic microbiome performed better than the uninoculated diploids when attacked by pathogens (Mehlferber et al, 2022), which may imply some differential recruitment of the microbiome in polyploids versus diploids. These latter two studies used synthetic “neopolyploids” (incipient polyploids), rather than established polyploids, and thus avoided the confounding effects of subsequent evolution following the whole‐genome duplication event, and yet they still observed variable responses to polyploidy, suggesting that the genotype of origin can alter the impact of whole‐genome duplication.…”

Section: Introductionmentioning

confidence: 99%

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Plant neopolyploidy and genetic background differentiate the microbiome of duckweed across a variety of natural freshwater sources

Anneberg,

Turcotte,

Ashman

2023

Molecular Ecology

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Whole‐genome duplication has long been appreciated for its role in driving phenotypic novelty in plants, often altering the way organisms interface with the abiotic environment. Only recently, however, have we begun to investigate how polyploidy influences interactions of plants with other species, despite the biotic niche being predicted as one of the main determinants of polyploid establishment. Nevertheless, we lack information about how polyploidy affects the diversity and composition of the microbial taxa that colonize plants, and whether this is genotype‐dependent and repeatable across natural environments. This information is a first step towards understanding whether the microbiome contributes to polyploid establishment. We, thus, tested the immediate effect of polyploidy on the diversity and composition of the bacterial microbiome of the aquatic plant Spirodela polyrhiza using four pairs of diploids and synthetic autotetraploids. Under controlled conditions, axenic plants were inoculated with pond waters collected from 10 field sites across a broad environmental gradient. Autotetraploids hosted 4%–11% greater bacterial taxonomic and phylogenetic diversity than their diploid progenitors. Polyploidy, along with its interactions with the inoculum source and genetic lineage, collectively explained 7% of the total variation in microbiome composition. Furthermore, polyploidy broadened the core microbiome, with autotetraploids having 15 unique bacterial taxa in addition to the 55 they shared with diploids. Our results show that whole‐genome duplication directly leads to novelty in the plant microbiome and importantly that the effect is dependent on the genetic ancestry of the polyploid and generalizable over many environmental contexts.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Plant neopolyploidy and genetic background differentiate the microbiome of duckweed across a variety of natural freshwater sources

Anneberg,

Turcotte,

Ashman

2023

Molecular Ecology

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“…Cold tolerance Arabidopsis accessions also showed changes in their microbiota composition [ 57 ]. In addition to host genotype effects on the Arabidopsis microbiota, ploidy levels, which vary in wild and managed populations, also affected microbial communities, as recently reported [ 129 , 149 ].…”

Section: Arabidopsis Features That Shape Its Microbial Communitiesmentioning

confidence: 80%

The Arabidopsis holobiont: a (re)source of insights to understand the amazing world of plant–microbe interactions

Poupin

Ledger

Roselló-Móra

et al. 2023

Environmental Microbiome

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As holobiont, a plant is intrinsically connected to its microbiomes. However, some characteristics of these microbiomes, such as their taxonomic composition, biological and evolutionary role, and especially the drivers that shape them, are not entirely elucidated. Reports on the microbiota of Arabidopsis thaliana first appeared more than ten years ago. However, there is still a lack of a comprehensive understanding of the vast amount of information that has been generated using this holobiont. The main goal of this review was to perform an in-depth, exhaustive, and systematic analysis of the literature regarding the Arabidopsis–microbiome interaction. A core microbiota was identified as composed of a few bacterial and non-bacterial taxa. The soil (and, to a lesser degree, air) were detected as primary microorganism sources. From the plant perspective, the species, ecotype, circadian cycle, developmental stage, environmental responses, and the exudation of metabolites were crucial factors shaping the plant–microbe interaction. From the microbial perspective, the microbe-microbe interactions, the type of microorganisms belonging to the microbiota (i.e., beneficial or detrimental), and the microbial metabolic responses were also key drivers. The underlying mechanisms are just beginning to be unveiled, but relevant future research needs were identified. Thus, this review provides valuable information and novel analyses that will shed light to deepen our understanding of this plant holobiont and its interaction with the environment.

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“…It has been observed that there is no difference in the establishment of the synthetic microbiome, neither its composition nor its diversity is significantly affected with respect to host ploidy. Also, at the gene expression level, autotetraploid plants show active defenses constitutively independent of pathogen colonization, whereas diploid plants show a high number of defense-related genes that are differentially expressed in the presence or absence of pathogens ( Mehlferber et al, 2022 ).…”

Section: Genomic Duplication and Biotic Stress Tolerancementioning

confidence: 99%

Impact of polyploidy on plant tolerance to abiotic and biotic stresses

et al. 2022

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Polyploidy, defined as the coexistence of three or more complete sets of chromosomes in an organism’s cells, is considered as a pivotal moving force in the evolutionary history of vascular plants and has played a major role in the domestication of several crops. In the last decades, improved cultivars of economically important species have been developed artificially by inducing autopolyploidy with chemical agents. Studies on diverse species have shown that the anatomical and physiological changes generated by either natural or artificial polyploidization can increase tolerance to abiotic and biotic stresses as well as disease resistance, which may positively impact on plant growth and net production. The aim of this work is to review the current literature regarding the link between plant ploidy level and tolerance to abiotic and biotic stressors, with an emphasis on the physiological and molecular mechanisms responsible for these effects, as well as their impact on the growth and development of both natural and artificially generated polyploids, during exposure to adverse environmental conditions. We focused on the analysis of those types of stressors in which more progress has been made in the knowledge of the putative morpho-physiological and/or molecular mechanisms involved, revealing both the factors in common, as well as those that need to be addressed in future research.

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Polyploidy and microbiome associations mediate similar responses to pathogens in Arabidopsis

Cited by 17 publications

References 71 publications

Plant neopolyploidy and genetic background differentiate the microbiome of duckweed across a variety of natural freshwater sources

Plant neopolyploidy and genetic background differentiate the microbiome of duckweed across a variety of natural freshwater sources

The Arabidopsis holobiont: a (re)source of insights to understand the amazing world of plant–microbe interactions

Impact of polyploidy on plant tolerance to abiotic and biotic stresses

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