Polysaccharide fractions from Fortunella margarita affect proliferation of Bifidobacterium adolescentis ATCC 15703 and undergo structural changes following fermentation

Abstract

In this study, the relationships between the proliferation effect of polysaccharide fractions from the citrus shrub Fortunella margarita on Bifidobacterium adolescentis ATCC 15703 and their resulting structural changes were investigated. Four polysaccharide fractions, FP20, FP40, FP60, and FP80, were obtained by graded precipitation at ethanol concentrations of 20%, 40%, 60% and 80%, respectively. The results showed that polysaccharide fractions, especially FP20, FP40, and FP60, enhanced the proliferation of B. adolescentis ATCC 15703 and their effects were better than those of FP80, inulin or glucose. Moreover, acetic acid was mainly produced during fermentation. After fermentation, the molecular weight of polysaccharide fractions decreased and the tightly structural chain conformations of FP20, FP40, and FP60 changed to highly branched structures. The prebiotic effect of these polysaccharide fractions might be related to their molecular weight and chain conformation. Thus, these polysaccharides could be used as potential prebiotics.

Structural characterization and in vitro fermentation by rat intestinal microbiota of a polysaccharide from Porphyra haitanensis

“…The molecular weights of the fermentation products were determined as described previously (Chen et al, 2019). After fermentation, the culture was centrifuged at 9600g for 20 min to remove the bacteria, then the supernatant was passed through a 0.22 μm water film (Jinteng, Tianjin, China).…”

The influence of gut microbiota on the rheological characterization of soy hull polysaccharide and mucin interactions

“…In addition, other studies have shown that polysaccharide fractions from the citrus shrub, Fortunella margarita , as a carbon source are superior to inulin and glucose in the proliferation of Bifidobacterium . 44 All the above results indicated that polysaccharides can be used as potential prebiotics. Moreover, studies have shown that Bifidobacterium and Lactobacillus not only do not degrade mucin glycoprotein, but also promote the secretion of intestinal mucin glycoprotein, and inhibit the adhesion of harmful bacteria such as Escherichia coli to mucous and intestinal epithelial cells.…”

Anticancer Potential of Post-Fermentation Media and Cell Extracts of Probiotic Strains: An In Vitro Study

“…The quantification of LA and SCFA profiles in PFM was performed according to the method presented by Chen et al [ 27 ], with some modifications. Determination of lactic, acetic, propionic, and butyric acids was conducted using high-performance liquid chromatography (HPLC).…”