Polysaccharides from <i>Ulva prolifera</i> O.F. Müller inhibit cell proliferation <i>via</i> activating MAPK signaling in A549 and H1650 cells

Yang, Juan; Wang, Ye Hua; Yin, Jiao; Liang, Hong; Shen, Song Dong

doi:10.1039/d1fo00294e

Cited by 7 publications

(7 citation statements)

References 40 publications

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“…Serving as both food and medicine, the anticancer activity of E. prolifera has been investigated for decades. The anti-carcinogenesis properties have been attributed to pheophytin and polysaccharides [ 8 , 9 , 10 ]. In contrast, there have been no reports on the bioactive peptides derived from E. prolifera .…”

Section: Discussionmentioning

confidence: 99%

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Preparation and Characterization of an Anticancer Peptide from Oriental Tonic Food Enteromorpha prolifera

Lin

Liu

Yan

et al. 2022

Foods

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Enteromorpha prolifera (E. prolifera), a tonic food in East Asian countries, is frequently studied for their pharmaceutical and healthcare applications. However, limited research has focused on antitumor peptides derived from this edible seaweed. In this study, we aimed to investigate the anticancer properties of peptides isolated from the hydrolysate of E. prolifera generated by a plethora of proteases including trypsin, papain, bromelain, and alkaline protease. The results showed that the hydrolysate produced by papain digestion exhibited remarkably stronger anticancer activity and was subjected to further purification by ultrafiltration and sequential chromatography. One heptapeptide, designated HTDT-6-2-3-2, showed significant antiproliferation activity towards several human cancer cell lines. The IC50 values for NCI-H460, HepG2, and A549 were 0.3686 ± 0.0935 mg/mL, 1.2564 ± 0.0548 mg/mL, and 0.9867 ± 0.0857 mg/mL, respectively. Moreover, results from flow cytometry confirmed that cell apoptosis was induced by HTDT-6-2-3-2 in a dose-dependent manner. The amino acid sequence for this heptapeptide, GPLGAGP, was characterized by Edman degradation and further verified by Liquid Chromatography-Tandem Mass Spectrometry. In silico analysis results suggested that XIAP could be a potential target for HTDT-6-2-3-2. Molecular docking simulation showed that HTDT-6-2-3-2 could occupy a shallow pocket in the BIR3 domain of XIAP, which is involved in the inhibitory effect of caspase-9 activation. In conclusion, this E. prolifera derived peptide exhibited strong anticancer properties, which could be explored for pharmaceutical applications.

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Section: Discussionmentioning

confidence: 99%

“…In another study, polysaccharides extracted from Ulva ( Enteromorpha ) prolifera O.F. Müller were reported to suppress H 2 O 2 -mediated MAPK activation and inhibit cell invasion by decreasing MMP-9 levels [ 10 ]. However, no research has been focused on the anticancer properties of peptides derived from E. prolifera .…”

Section: Introductionmentioning

confidence: 99%

Preparation and Characterization of an Anticancer Peptide from Oriental Tonic Food Enteromorpha prolifera

Lin

Liu

Yan

et al. 2022

Foods

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“…Furthermore, polysaccharides from Ulva prolifera O.F. Müller contributed to suppression of H 2 O 2 -induced cell invasion by inhibiting MYC-mediated MMP-9 gene transcription via MAPK signaling in A549 and NCI-H1650 lung cancer cells [ 562 ]. In a gastric cancer cell model, polysaccharides extracted from different parts of Dendrobium huoshanense downregulated c-Myc gene expression in MFC cells and promoted p53 transcription, thereby enhancing p53-dependent apoptosis [ 563 ].…”

Section: Myc Modulators As Cancer Treatmentsmentioning

confidence: 99%

MYC Oncogene: A Druggable Target for Treating Cancers with Natural Products

Chan,

Zhang,

et al. 2024

Aging and disease

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Various diseases, including cancers, age-associated disorders, and acute liver failure, have been linked to the oncogene, MYC . Animal testing and clinical trials have shown that sustained tumor volume reduction can be achieved when MYC is inactivated, and different combinations of therapeutic agents including MYC inhibitors are currently being developed. In this review, we first provide a summary of the multiple biological functions of the MYC oncoprotein in cancer treatment, highlighting that the equilibrium points of the MYC/MAX, MIZ1/MYC/MAX, and MAD (MNT)/MAX complexes have further potential in cancer treatment that could be used to restrain MYC oncogene expression and its functions in tumorigenesis. We also discuss the multifunctional capacity of MYC in various cellular cancer processes, including its influences on immune response, metabolism, cell cycle, apoptosis, autophagy, pyroptosis, metastasis, angiogenesis, multidrug resistance, and intestinal flora. Moreover, we summarize the MYC therapy patent landscape and emphasize the potential of MYC as a druggable target, using herbal medicine modulators. Finally, we describe pending challenges and future perspectives in biomedical research, involving the development of therapeutic approaches to modulate MYC or its targeted genes. Patients with cancers driven by MYC signaling may benefit from therapies targeting these pathways, which could delay cancerous growth and recover antitumor immune responses.

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“…Washed cells are further incubated with 10 mM CM-DCFH at RT for 30 min. Relative fluorescent intensity of the fluorophore can be detected at ex/em 485/530 nm or using a fluorescence microscope [ 75 ].…”

Section: Analysis Of Ros Productionmentioning

confidence: 99%

“…Twenty μl of the lysate is then mixed with 100 μl luciferin and the luminescence is measured. Results can be described as relative luciferase activity (fold difference in comparison with negative control) [ 75 , 92 , 114 , 128 ].…”

Section: Assays On Nf-κb Proteinmentioning

confidence: 99%

Understanding immune-modulatory efficacy in vitro

Khatua

Simal-Gándara

Acharya

2022

Chemico-Biological Interactions

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Boosting or suppressing our immune system represents an attractive adjunct in the treatment of infections including SARS-CoV-2, cancer, AIDS, malnutrition, age related problems and some inflammatory disorders. Thus, there has been a growing interest in exploring and developing novel drugs, natural or synthetic, that can manipulate our defence mechanism. Many of such studies, reported till date, have been designed to explore effect of the therapeutic on function of macrophages, being a key component in innate immune system. Indeed, RAW264.7, J774A.1, THP-1 and U937 cell lines act as ideal model systems for preliminary investigation and selection of dose for in vivo studies. Several bioassays have been standardized so far where many techniques require high throughput instruments, cost effective reagents and technical assistance that may hinder many scholars to perform a method demanding compilation of available protocols. In this review, we have taken an attempt for the first time to congregate commonly used in vitro immune-modulating techniques explaining their principles. The study detected that among about 40 different assays and more than 150 sets of primers, the methods of cell proliferation by MTT, phagocytosis by neutral red, NO detection by Griess reaction and estimation of expression of TLRs, COX-2, iNOS, TNF-α, IL-6 and IL-1β by PCR have been the most widely used to screen the therapeutics under investigation.

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Polysaccharides from Ulva prolifera O.F. Müller inhibit cell proliferation via activating MAPK signaling in A549 and H1650 cells

Abstract: Reactive oxygen species (ROS), especially hydrogen peroxide (H2O2), has recently been reported to cause a significant increase in the production and expression of matrix metalloproteinase (MMPs), which are closely correlated...

Cited by 7 publications

References 40 publications

Preparation and Characterization of an Anticancer Peptide from Oriental Tonic Food Enteromorpha prolifera

Preparation and Characterization of an Anticancer Peptide from Oriental Tonic Food Enteromorpha prolifera

MYC Oncogene: A Druggable Target for Treating Cancers with Natural Products

Understanding immune-modulatory efficacy in vitro

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