In this study, we focus on the preparation, microstructures, and properties of polyurethane (PU) elastomers by using polyether glycol, toluene diisocyanate, and extender chain reagent. We find that the elastomers prepared by using extender chain reagent exhibit the most excellent mechanical properties. Furthermore, by an optimum synthetic route, hard segments can be formed uniformly in the soft segments, leading to the perfect micro-phase separation. We also find that the relative molecular weight of polyether glycol can affect the mechanical properties of the synthesized PU elastomers, i.e., the more relative molecular weight, the more soft segments, and the smaller tensile strength, rupture strength, and rigidity.