Pomegranate (Punica granatum Linn.) leaves attenuate disturbed glucose homeostasis and hyperglycemia mediated hyperlipidemia and oxidative stress in streptozotocin induced diabetic rats

Patel, Ankita; Bandawane, D. D.; Mhetre, Nilam Kiran

doi:10.1016/j.eujim.2014.03.009

Cited by 45 publications

(31 citation statements)

References 49 publications

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“…Thus, in the present study, STZ was used for induction of diabetes in rats. Administration of STZ led to significant increase in blood glucose level which was lowered on treatment with MPGL thus confirming the antihyperglycemic activity of the extract as reported by earlier established studies 13, 24. However, up to 4 weeks no significant difference was observed in the glucose level of MPGL treated groups as compared to diabetic group.…”

Section: Discussionsupporting

confidence: 86%

Attenuation of diabetic nephropathy in streptozotocin-induced diabetic rats by Punica granatum Linn. leaves extract

Mestry

Dhodi

Kumbhar

et al. 2017

Journal of Traditional and Complementary Medicine

134

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With an objective to develop Complementary and Alternative Medicine for the treatment of diabetic nephropathy, the present study investigated the protective effects of methanolic extract of Punica granatum leaves (MPGL) in streptozotocin-induced diabetic nephropathy. Diabetic nephropathy has become a leading cause of end stage renal failure worldwide. P. granatum, due to its anti-diabetic, anti-inflammatory and antioxidant activities may retard the progression of diabetic nephropathy. In this study, diabetes was induced by a single injection of streptozotocin (STZ, 45 mg/kg, i.p.) in rats. STZ-diabetic rats were treated with oral doses of MPGL (100, 200 and 400 mg/kg) for 8 weeks. At the end of the experimental period, body and kidney weight and blood glucose levels were determined. Serum and urine parameters were investigated. Antioxidant enzymes and lipid peroxide levels were determined in the kidney along with histopathological examination of the same. MPGL significantly increased body weight, lowered blood glucose levels and ameliorated kidney hypertrophy index in the STZ-diabetic rats. The extract also decreased the levels of creatinine, blood urea nitrogen, total cholesterol, triglycerides, advanced glycation end products and albumin in serum and urine, respectively. MPGL significantly increased the antioxidant parameters in the kidney. Histological evaluation revealed that MPGL treated STZ-diabetic rats demonstrated reduced vacuolar degeneration of tubules; periodic acid Schiff base (PAS) positivity staining intensity in glomeruli and basement membrane thickening. Present findings provide experimental evidence that MPGL has potential antioxidant, antihyperglycemic and anti-glycation activities which might be helpful in slowing the progression of diabetic nephropathy.

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Section: Discussionsupporting

confidence: 86%

Attenuation of diabetic nephropathy in streptozotocin-induced diabetic rats by Punica granatum Linn. leaves extract

Mestry

Dhodi

Kumbhar

et al. 2017

Journal of Traditional and Complementary Medicine

134

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“…This was clearly evidenced by the increased levels of plasma insulin in diabetic rats treated with plant extract which may stimulate insulin secretion from regenerated b-cells or caused the release of insulin from the residual b-cells. Some antidiabetic plants may exert their action by stimulating the function or number of b-cells, and thus increasing insulin release (Daisy et al 2009;Patel et al 2014). Another parameter is the a-glucosidase inhibitors, such as acarbose, voglibose and miglitol, which is widely used either alone or in combination with insulin secretagogues in patients with type 2 diabetes (Bedekar et al 2010).…”

Section: Discussionmentioning

confidence: 99%

Healing effects of sumac (Rhus coriaria) in streptozotocin-induced diabetic rats

Doğan

Çelik

2016

Pharmaceutical Biology

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Context Sumac [Rhus coriaria L. (RC) (Anacardiaceae)] is used as a folk medicine in the treatment of diabetes in Turkey. Objective This study investigates the in vivo healing and protective effects of lyophilized extract sumac against streptozotocin (STZ)-induced diabetic complications. Materials and methods Toxicity test was conducted in three different dosages (250, 500 and 1000 mg/kg of plant extracts, respectively). Six groups of seven rats each were used in experiments. Groups were designed as Normal control, Diabetic (DM), DM + AC-20 mg/kg, DM + Extract-100 mg/ kg, DM + Extract 250 mg/kg and DM + Extract 500 mg/kg group. Experimental diabetes [50 mg/kg, intraperitoneal (i.p.)] was induced by STZ. The effects of oral administration of the extract for 21 d on the level of serum glucose, insulin, C-peptide, lipid profile (LP), hepatic and renal damage biomarkers (HRDB), diabetic serum biomarkers (DSB), glycosylated haemoglobin (HbA1c), antioxidant defence system constituents (ADSCs), malondialdehyde (MDA) and a-glucosidase activity in small intestine tissue were evaluated. Results The extract decreased the levels of blood glucose in diabetic groups (an average of 31%). Triglyceride, total cholesterol, high-density lipoprotein and low-density lipoprotein levels were balanced by plant extract (500 mg/kg) supplementation in the diabetic group. Decreased levels of aspartate aminotransferase (89%), alanine aminotransferase (91%), lactate dehydrogenase (35%), alkaline phosphatase (47%), creatinine (25%) and urea (29%) were detected in plant extract (500 mg/kg) supplemented diabetic group. Additionally, a considerable increase in the HRDB, DSB, LP, MDA and fluctuated ADSC levels were restored in RC-extract supplemented groups. Conclusion RC lyophilized extract has a healing effect on diabetes and diabetes-related complications.ARTICLE HISTORY

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“…The solution was well vortexed and absorbance was measured against reagent blank at 510 nm. The total flavonoid content (mg/g) was determined from the calibration curve of quercetin and expressed as mg quercetin equivalents [27,28].…”

Section: Determination Of Total Flavonoidsmentioning

confidence: 99%

Correlation of the Cytotoxic and Antioxidant Activities of Moroccan Pomegranate (Punica Granatum) with Phenolic and Flavonoid Contents

Eddebbagh¹,

Massoudi²,

Abourriche³

et al. 2016

JPP

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Abstract:The Pomegranate (Punica Granatum), which belongs to the Lythraceae family, has been used for centuries in traditional Greco-Arab and Islamic medicine of its vermifuge properties and also to treat various diseases. The aim of this research was to investigate the cytotoxicity and antioxidant activities of Moroccan Pomegranate (peel, leaves, branches, flowers and corolla). Further, the biological activities were correlated with phytochemical contents of the plant extracts. Methanolic extract from different parts of Punica Granatum was assessed for its antiproliferative activity in two human cancer (breast and colon) cells lines (MBA-MD 231 and HT-29), through MTT (3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide) bioassay using cell viability and cytotoxicity indices. DPPH (2,2-diphenyl-1-picrylhydrazyl) assay was conducted to screen the antioxidant property of the extracts together with its phenolic and flavonoids content were evaluated, as well. The methanolic extract of Punica Granatum (peel, leaves, branches, flowers and corolla) showed the highest antiproliferative activity on MBA-MD 231 (IC 50 was 133.53~233.32 μg/mL) and HT-29 (IC 50 was 127.58~203.24 μg/mL) cells. Antioxidants contents are distributed as follows: peel > leaf > flower > corolla > branches. The inhibitory activities required for decreasing initial DPPH by 50% are 8.27, 9.9, 10.06, 11.67 and 13.28 µg/mL, respectively. These results are in correlation with polyphenols content from corolla, peel, leaves, flower and branches are 120.7, 115, 96.65, 90.73 and 64.67 mg GAE/g dw (mg gallic acid equivalents per g dry weight) and flavonoids are 188.8, 221.7, 180.2, 193.7 and 158.5 mg QE/g dw (mg quercetin equivalents per g dry weight). Our results show that the peel, flowers, corolla, leaves and branches of Moroccan Pomegranate may contain a lot of bioactive compounds which are responsible for strong antioxidant and cytotoxicity activities observed here. Our finding indicates the possibility of using the extracts of this plant as source of natural antioxidant and anticancer mainly for its abundant phenolic and flavonoid contents.

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Pomegranate (Punica granatum Linn.) leaves attenuate disturbed glucose homeostasis and hyperglycemia mediated hyperlipidemia and oxidative stress in streptozotocin induced diabetic rats

Cited by 45 publications

References 49 publications

Attenuation of diabetic nephropathy in streptozotocin-induced diabetic rats by Punica granatum Linn. leaves extract

Attenuation of diabetic nephropathy in streptozotocin-induced diabetic rats by Punica granatum Linn. leaves extract

Healing effects of sumac (Rhus coriaria) in streptozotocin-induced diabetic rats

Correlation of the Cytotoxic and Antioxidant Activities of Moroccan Pomegranate (Punica Granatum) with Phenolic and Flavonoid Contents

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