Poncirin and its metabolite ponciretin attenuate colitis in mice by inhibiting LPS binding on TLR4 of macrophages and correcting Th17/Treg imbalance

Kang, Geum-Dan; Kim, Dong-Hyun

doi:10.1016/j.jep.2016.05.044

Cited by 54 publications

(22 citation statements)

References 29 publications

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“…In order to better study the mechanisms of severe asthma, we urgently need to build a neutrophil-predominant severe asthma model. While OVA is a classic asthma model allergen, exposure to LPS is also known to lead to an increased risk of asthma-like symptoms [ 17 ] and the onset of asthma exacerbations [ 18 , 19 ]. Meanwhile, the development of allergic asthma is strongly associated with the exposure to HDM [ 20 , 21 ].…”

Section: Discussionmentioning

confidence: 99%

MBD2 Regulates Th17 Cell Differentiation and Experimental Severe Asthma by Affecting IRF4 Expression

Jia

Wang

Sun

et al. 2017

Mediators of Inflammation

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Th17 cells and IL-17 participate in airway neutrophil infiltration characteristics in the pathogenesis of severe asthma. Methyl-CpG binding domain protein 2 (MBD2) expression increased in CD4+ T cells in peripheral blood samples of asthma patients. However, little is known about that epigenetic regulation of MBD2 in both immunological pathogenesis of experimental severe asthma and CD4+ T cell differentiation. Here, we established a neutrophil-predominant severe asthma model, which was characterized by airway hyperresponsiveness (AHR), BALF neutrophil granulocyte (NEU) increase, higher NEU and IL-17 protein levels, and more Th17 cell differentiation. In the model, MBD2 and IRF4 protein expression increased in the lung and spleen cells. Under overexpression or silencing of the MBD2 and IRF4 gene, the differentiation of Th17 cells and IL-17 secretion showed positive changes. IRF4 protein expression showed a positive change with overexpression or silencing of the MBD2 gene, whereas there was no significant difference in the expression of MBD2 under overexpression or silencing of the IRF4 gene. These data provide novel insights into epigenetic regulation of severe asthma.

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Section: Discussionmentioning

confidence: 99%

MBD2 Regulates Th17 Cell Differentiation and Experimental Severe Asthma by Affecting IRF4 Expression

Jia

Wang

Sun

et al. 2017

Mediators of Inflammation

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“…Saikosaponin A inhibited LPS induced nuclear factor- κ B (NF- κ B) and interferon regulatory factor 3 (IRF3) activation and the production of tumor necrosis factor- α (TNF- α ), interleukin- (IL-) 1 β , IL-6 and regulated upon activation normal T-cell expressed and secreted (RANTES) in primary mouse macrophages [30]. Poncirin also inhibited the activation of macrophages stimulated with LPS through the inhibition of LPS binding on Toll-like receptor-4 (TLR4) of macrophages [31]. Paeoniflorin exerted anti-inflammatory effects on primary human hepatic sinusoidal endothelial cells through blocking IL-8 secretion via downregulation of extracellular signal-regulated kinase (ERK) 1/2 and Akt phosphorylation [32].…”

Section: Discussionmentioning

confidence: 99%

Shugan Xiaozhi Decoction Attenuates Nonalcoholic Steatohepatitis by Enhancing PPARα and L‐FABP Expressions in High‐Fat‐Fed Rats

Xing

Zhang

et al. 2016

Evidence-Based Complementary and Alternative Medicine

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This study aimed to investigate the effects of Shugan Xiaozhi decoction (SX) on nonalcoholic steatohepatitis (NASH) induced by high-fat diet in rats. The rats were randomly divided into 6 groups, namely, control, model, fenofibrate, and three different dosage of SX (10, 20, and 40 g/kg/day, p.o.). After establishing the NASH model, at 8 weeks of the experiment, treatments were administrated intragastrically to the fenofibrate and SX groups. All rats were killed after 4 weeks of treatment. Compared with the model group, alanine aminotransferase (ALT), aspartate aminotransferase (AST), free fatty acid (FFA), total cholesterol (TC), triacylglycerol (TG), and low-density lipoprotein cholesterol (LDL) serum in the serum were significantly reduced in all SX treatment groups in a dose-dependent manner. Evidence showed that SX could protect the liver by upregulating the gene and protein expressions of peroxisome proliferator-activated receptor alpha (PPARα) and liver fatty acid binding protein (L-FABP) in a dose-dependent manner. Chemical constituents of SX were further analyzed by ultraperformance liquid chromatography coupled with electrospray ionization mass spectrometry (UPLC-ESI-MS) and 30 chemicals in the ethanolic extract were tentatively identified. To conclude, our results clearly indicated that SX could protect liver functions and relieve hepatic steatosis and inflammation.

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“…Among the various types of identified phytochemicals in HECA, the therapeutic effects of coumarins (e.g., scopoletin and isoimperatorin), flavonoids (e.g., hesperidin, isoquercetin, miscanthoside, naringin, naringenin, nobiletin, poncirin, quercetin, rutin, tangeritin, vitexin), lignans (e.g., acanthoside D), phenolics (e.g., ferulic acid), and sesquiterpenes (e.g., abscisic acid) have been reported against UC in animal models and human studies [12,17,[24][25][26][27][28][29][30][31][32][33][34]. These phytochemicals inhibited the overproduction of inflammatory mediators (e.g., TNF-α, IL-1β, IL-4, IL-6, IL-17, and continued prostaglandin E 2 ) and oxidative stress (e.g., nitric oxide, myeloperoxidase, superoxide dismutase, and malonaldehyde) while improving colonic structural damage (e.g., occludin, claudin-1, and zona occludens protein-1).…”

Section: Resultsmentioning

confidence: 99%

Preventive Effects of an UPLC-DAD-MS/MS Fingerprinted Hydroalcoholic Extract of Citrus aurantium in a Mouse Model of Ulcerative Colitis

Hwang

Yeul

2018

Planta Med

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Although traditionally used to improve indigestion, diarrhea, dysentery, and constipation, the therapeutic effects of on intestinal inflammation remain unclear. The aim of this study was to evaluate the beneficial effects and to identify the active components of a hydroalcoholic extract of (HECA) on ulcerative colitis. HECA was prepared with 70% ethanol solution in water and extracted at 37 °C for 12 h in triplicate, filtered through a sieve, and lyophilized. Phytochemical identification of HECA was performed by ultra-performance liquid chromatography-diode array detector-tandem mass spectrometry (UPLC-DAD-MS/MS). Animals were randomly assigned to one of four groups based on the treatment conditions. Ulcerative colitis was induced by administration of 2.5% dextran sodium sulfate (DSS) in drinking water for 5 d. Body weight, clinical signs, colon length, pro-inflammatory cytokine expression levels, and histopathological findings were evaluated. In UPLC-DAD-MS/MS analysis, the identified phytochemical components of HECA included four alkaloids, seven coumarins, 18 flavonoids, two lignans, two phenolics, and 10 terpenoids. HECA markedly protected against body weight loss and colon shortening. In pathological examination, HECA alleviated DSS-related mucosal inflammatory lesions in the colon. Moreover, HECA markedly reduced the expression levels of interleukin-6, interferon-, tumor necrosis factor-, and monocyte chemotactic protein-1 in colonic inflammation. Taken together, HECA has potential to relieve mucosal inflammation in the colon, suggesting that the putative active ingredients are responsible for the anti-ulcerative effects.

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Poncirin and its metabolite ponciretin attenuate colitis in mice by inhibiting LPS binding on TLR4 of macrophages and correcting Th17/Treg imbalance

Cited by 54 publications

References 29 publications

MBD2 Regulates Th17 Cell Differentiation and Experimental Severe Asthma by Affecting IRF4 Expression

MBD2 Regulates Th17 Cell Differentiation and Experimental Severe Asthma by Affecting IRF4 Expression

Shugan Xiaozhi Decoction Attenuates Nonalcoholic Steatohepatitis by Enhancing PPARα and L‐FABP Expressions in High‐Fat‐Fed Rats

Preventive Effects of an UPLC-DAD-MS/MS Fingerprinted Hydroalcoholic Extract of Citrus aurantium in a Mouse Model of Ulcerative Colitis

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