Pontibacter toksunensis sp. nov., isolated from soil, and emended descriptions of Pontibacter roseus and Pontibacter akesuensis

Abstract

A Gram-reaction-negative, rod-shaped, non-motile, red-pigmented bacterial strain, designated ZLD-7T, was isolated from a soil sample collected from an arid area in Xinjiang Province in north-west China, and characterized by using a polyphasic taxonomic approach. 16S rRNA gene sequence analysis showed that strain ZLD-7T was a member of the genus Pontibacter in the family Cytophagaceae , with sequence similarities of 93.7–96.2 % to type strains of other Pontibacter species. The only isoprenoid quinone of strain ZLD-7T was MK-7, and its major cellular fatty acids were summed feature 4 (anteiso-C17 : 1 B and/or iso-C17 : 1 I), iso-C15 : 0, iso-C17 : 0 3-OH and summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c). The polar lipid profile consisted of phosphatidylethanolamine, one unidentified aminolipid and four unidentified polar lipids. The DNA G+C content was 47.8 mol%. On the basis of the evidence presented, it is proposed that strain ZLD-7T represents a novel species of the genus Pontibacter , for which the name Pontibacter toksunensis sp. nov. is proposed. The type strain is ZLD-7T ( = CCTCC AB 208003T = KCTC 23984T). Emended descriptions of Pontibacter roseus and Pontibacter akesuensis are also proposed.

Pontibacter diazotrophicus sp. nov., a Novel Nitrogen-Fixing Bacterium of the Family Cytophagaceae

“…The major fatty acids include iso-C 15∶0 (10.9%), C 16∶1 ω5c (14.3%), summed feature 3 (containing C 16∶1 ω6c and/or C 16∶1 ω7c) (21.6%) and summed feature 4 (comprising anteiso-C 17∶1 B and/or iso-C 17∶1 I) (31.9%) (Table 1). All these chemotaxonomic properties of the strain H4X T are consistent with those of other members of the genus Pontibacter described so far [22], [23].…”

Pontibacter flavimaris sp. nov., of the family Hymenobacteraceae, isolated from marine sediment

“…However, the close relative P. litorisediminis KCTC 52252 T showed that α-chymotrypsin, β-glucosidase, α-mannosidase, α-galactosidase and cystine arylamidase were weakly positive, α-glucosidase, N-acetyl-β-glucosaminidase, acid phosphatase, naphthol-AS-BI-phosphohydrolase, alkaline phosphatase, esterase lipase (C8), esterase (C4), leucine arylamidase, trypsin and valine arylamidase were positive, and β-galactosidase, α-fucosidase, β-glucuronidase and lipase (C14) were negative. MK-7 was identified as the major respiratory quinone of strain S10-8 T , similar to P. actiniarum KCTC 12367 T , P. korlensis CCTCC AB206081 T , P. akesuensis CCTCC AB206086 T , P. litorisediminis KCTC 52252 T and most other members of the genus Pontibacter [1][2][3][4][5][6][7][8][9][10][11][12][13][14][15][16][17][18].…”

Pontibacter mucosus sp. nov., isolated from hexachlorocyclohexane-contaminated pond sediment

“…This is because obtaining the type strain of P. niistensis NII-0905 T from the two culture collections where it was deposited (the NCIM in Pune, India, and the CCTCC in Wuhan, China) and from the authors who described the species (Dastager et al, 2010) was unsuccessful. The authors were unable to revive the strain and even the two culture collection centers confirmed that this strain was not available (Zhang et al, 2013;Singh et al, 2015). The DNA-DNA hybridization was carried out between strain PB3 T , P. chinhatensis LP51 T and P. korlensis X-14 T , which showed sequence similarity >97 % at the 16S rRNA gene sequence level.…”