Porcine myelomonocytic markers: summary of the second international swine CD workshop

Domı́nguez, Javier; Ezquerra, Ángel; Alonso, Fernando; McCullough, Ken; Summerfield, Artur; Bianchi, A.T.J.; Zwart, Rob; Kim, Y.B; Blecha, Frank; Eicher, S.D.; Murtaugh, Michael P.; Pampusch, Mary S.; Burger, K

doi:10.1016/s0165-2427(97)00109-8

Cited by 28 publications

(12 citation statements)

References 6 publications

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“…1B). Blood DC were firstly identified as CD172a + lin − cells, and CD172a high cells were excluded since it has been shown that Tuk4 antibody for CD14 may not identify all monocytes4445. Two populations of CD172a + lin − cells could be identified; CD4 − CD1 − and CD4 − CD1 + ; while pDC were identified as CD4 + CD1 − cells (Fig.…”

Section: Resultsmentioning

confidence: 99%

CD1− and CD1+ porcine blood dendritic cells are enriched for the orthologues of the two major mammalian conventional subsets

Edwards

Everett

Pedrera

et al. 2017

Sci Rep

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Conventional dendritic cells (cDC) are professional antigen-presenting cells that induce immune activation or tolerance. Two functionally specialised populations, termed cDC1 and cDC2, have been described in humans, mice, ruminants and recently in pigs. Pigs are an important biomedical model species and a key source of animal protein; therefore further understanding of their immune system will help underpin the development of disease prevention strategies. To characterise cDC populations in porcine blood, DC were enriched from PBMC by CD14 depletion and CD172a enrichment then stained with lineage mAbs (Lin; CD3, CD8α, CD14 and CD21) and mAbs specific for CD172a, CD1 and CD4. Two distinct porcine cDC subpopulations were FACSorted CD1− cDC (Lin−CD172+ CD1−CD4−) and CD1+ cDC (Lin−CD172a+ CD1+ CD4−), and characterised by phenotypic and functional analyses. CD1+ cDC were distinct from CD1− cDC, expressing higher levels of CD172a, MHC class II and CD11b. Following TLR stimulation, CD1+ cDC produced IL-8 and IL-10 while CD1− cDC secreted IFN-α, IL-12 and TNF-α. CD1− cDC were superior in stimulating allogeneic T cell responses and in cross-presenting viral antigens to CD8 T cells. Comparison of transcriptional profiles further suggested that the CD1− and CD1+ populations were enriched for the orthologues of cDC1 and cDC2 subsets respectively.

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Section: Resultsmentioning

confidence: 99%

CD1− and CD1+ porcine blood dendritic cells are enriched for the orthologues of the two major mammalian conventional subsets

Edwards

Everett

Pedrera

et al. 2017

Sci Rep

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“…The mAb anti-hCD14 cross-react with porcine CD14 expressed on alveolar macrophages [107], on 60-70% swine monocytes but few granulocytes (6-13%) [69]. Inversely mAb anti-swine CD14 cross-react with human monocytes and granulocytes, in a pattern similar to that of human CD14 [209].…”

Section: Cd14mentioning

confidence: 89%

“…Inversely, mAb anti-swine CD11b defines a cluster of differentiation that corresponds to the expression of CD11b similar to that observed on human cells but with a different MW so that they were designed as wCD11R3 [67,69,209,220].…”

Section: The 2 Integrin Subfamily Cd11a-c/cd18mentioning

confidence: 99%

Membrane markers of the immune cells in swine: an update

Piriou-Guzylack¹,

2008

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-Besides their breeding value, swine are increasingly used as biomedical models. As reported in three international swine clusters of differentiation (CD) workshops and in the animal homologue section of the last workshop for the determination of human leukocyte differentiation antigens (HLDA 8), characterisation of leukocyte surface antigens by monoclonal antibodies and other molecular studies have determined the cell lineages and blood leukocyte subsets implicated in the immune response, including cell adhesion molecules involved in cell trafficking. This review focusses on the current state of knowledge of porcine leukocyte differentiation and major histocompatibility complex (SLA) molecules. Examples of porcine particularities such as the double-positive T lymphocytes with the phenotype CD4 + CD8 low and CD4 − CD8 low T cell subsets and the persistence of SLA class II after T-lymphocyte activation are illustrated, as well as the shared characteristics of the Artiodactyla group, such as the high proportion of TcR (T cell receptor) T cells in blood and other lymphoid tissues. Furthermore, discrepancies between swine and humans, such as CD16 expression on dendritic cells and CD11b (wCD11R1) tissue distribution are outlined. The rapidly growing information should facilitate manipulation of the swine immune system towards improving disease control, and open new avenues for biomedical research using the pig as a model. cluster of differentiation (CD) / monoclonal antibody / immune system / histocompatibility system / pig

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“…After centrifugation at 400 g for 15 min, pelleted cells were washed once with NaCl/P i . Cultures were examined immuno-histochemically by staining with a specific antibody for porcine macrophages (mAb 517.2) [23]. The percentage of Kupfer cells present in the hepatocyte cultures was around 5±8%.…”

Section: Preparation Of Porcine Hepatocytesmentioning

confidence: 99%

Pig MAP/ITIH4 and haptoglobin are interleukin‐6‐dependent acute‐phase plasma proteins in porcine primary cultured hepatocytes

González‐Ramón

Hoebe²,

Álava

et al. 2000

European Journal of Biochemistry

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The acute-phase expression of pig MAP (major acute-phase protein)/ITIH4 (inter-a-trypsin inhibitor heavy chain 4) and haptoglobin were analysed in primary cultures of isolated pig hepatocytes in response to recombinant human (rh) cytokines: tumor necrosis factor-a (TNF-a), interleukin-1 (IL-1), interleukin-6 (IL-6), as well as to bacterial lipopolysaccharide (LPS). Analysis of pig MAP/ITIH4 and haptoglobin mRNAs was carried out by RT-PCR amplification. Secreted proteins from the cytokine-treated hepatocytes were quantified by immunochemical techniques. Time-course and dose±response experiments show that pig MAP/ITIH4 and haptoglobin belong to the type II acute-phase proteins, as they are specifically induced by rhIL-6 and not by rhTNF-a or rhIL-1. Stimulation of cultured pig hepatocytes with rhIL-6 for 48 h at doses of 1000 U´mL 21 showed a fourfold to fivefold increase in pig MAP/ITIH4 concentration in the medium, while the concentration of haptoglobin only increased twofold. A similar increase in the concentration of pig MAP/ITIH4 was also observed in media of LPS-treated hepatocytes with the simultaneous generation of IL-6 by the Kupfer cells present in the cultures. Albumin secretion decreased after stimulation with doses of 100 or 1000 U´mL 21 rhTNF-a, rhIL-1 or rhIL-6. Therefore, it can be concluded that pig MAP/ITIH4 behaves as a major acutephase protein produced by porcine hepatocytes under the effect of inflammatory cytokines.Keywords: acute-phase proteins; cytokines; hepatocytes; inter-a-trypsin inhibitor (IaI); ITIH4.The acute-phase response of an organism to infection, tissue injury and inflammation consists, apart from local reactions, of a systemic response characterized by fever, leukocytosis, secretion of glucocorticoids, activation of complement and clotting cascades, and a dramatic change in the concentration of some plasma proteins denoted acute-phase proteins [1±3]. Activated macrophages or blood monocytes release inflammation-related cytokines, such as tumor necrosis factor (TNF), interleukin-1 (IL-1) and interleukin-6 (IL-6), which initiate the acute-phase response [4]. Inflammation-related cytokines act as primary stimulators of acute-phase proteins gene expression in the liver, which is also modulated by glucocorticoids and growth factors. They are grouped in two major categories: (a) gp130 signalling cytokines (IL-6-type cytokines): IL-6, IL-11, leukaemia inhibitory factor, oncostatin M, ciliary neurotrophic factor and cardiotropin; and (b) IL-1-type cytokines, IL-1a, IL-1b, and TNF (a and b) [3,4].Acute-phase proteins have been studied extensively in humans and in the rat. Several studies in vivo and in vitro have been performed to analyse the response of acute-phase proteins to cytokine stimulation and IL-6 has been recognized as the principal regulator of most acute-phase protein genes in both species [5].Not much is known about the acute-phase protein response in domestic animals. Haptoglobin, C-reactive protein and serum amyloid A have been reported to be acute-phase proteins in pig...

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Porcine myelomonocytic markers: summary of the second international swine CD workshop

Cited by 28 publications

References 6 publications

CD1− and CD1+ porcine blood dendritic cells are enriched for the orthologues of the two major mammalian conventional subsets

CD1− and CD1+ porcine blood dendritic cells are enriched for the orthologues of the two major mammalian conventional subsets

Membrane markers of the immune cells in swine: an update

Pig MAP/ITIH4 and haptoglobin are interleukin‐6‐dependent acute‐phase plasma proteins in porcine primary cultured hepatocytes

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