Porous ceramic titanium dioxide scaffolds promote bone formation in rabbit peri-implant cortical defect model

Haugen, Håvard Jostein; Monjo, Marta; Rubert, Marina; Verket, Anders; Lyngstadaas, Ståle Petter; Ellingsen, Jan Eirik; Rønold, Hans Jacob; Wohlfahrt, Johan Caspar

doi:10.1016/j.actbio.2012.09.009

Cited by 85 publications

(58 citation statements)

References 65 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…189,190 Also, 3D microporous NiTi and Ti scaffolds have been produced by powder metallurgy obtaining a hydrophilic surface to facilitate the deposition of HA and stimulate cell attachment and proliferation. 190 Osteoconductive properties and biological performance of Ti and TiO 2 scaffolds have been investigated 191, 192 in rabbit tibia peri-implant osseous defects and rabbit peri-implant defects, showing osteoconductivity and osseointegration. 69, 70 The fixation of porous Ti implants in rabbits is shown in Figure 7.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Scaffold Design for Bone Regeneration

Polo-Corrales¹,

Latorre-Esteves²,

Ramı́rez-Vick³

2014

j. nanosci. nanotech.

788

502

View full text Add to dashboard Cite

The use of bone grafts is the standard to treat skeletal fractures, or to replace and regenerate lost bone, as demonstrated by the large number of bone graft procedures performed worldwide. The most common of these is the autograft, however, its use can lead to complications such as pain, infection, scarring, blood loss, and donor-site morbidity. The alternative is allografts, but they lack the osteoactive capacity of autografts and carry the risk of carrying infectious agents or immune rejection. Other approaches, such as the bone graft substitutes, have focused on improving the efficacy of bone grafts or other scaffolds by incorporating bone progenitor cells and growth factors to stimulate cells. An ideal bone graft or scaffold should be made of biomaterials that imitate the structure and properties of natural bone ECM, include osteoprogenitor cells and provide all the necessary environmental cues found in natural bone. However, creating living tissue constructs that are structurally, functionally and mechanically comparable to the natural bone has been a challenge so far. This focus of this review is on the evolution of these scaffolds as bone graft substitutes in the process of recreating the bone tissue microenvironment, including biochemical and biophysical cues.

show abstract

Section: Methodsmentioning

confidence: 99%

“…Also, Haugen et al 191 reported in vitro results that showed cell viability of the mouse osteoblastic cell line MC3T3-E1, no cytotoxic effects from the TiO 2 -HA scaffolds were found. It was also reported that healing occurred when this scaffold was implanted into rabbit defects.…”

Section: Methodsmentioning

confidence: 99%

Scaffold Design for Bone Regeneration

Polo-Corrales¹,

Latorre-Esteves²,

Ramı́rez-Vick³

2014

j. nanosci. nanotech.

788

502

View full text Add to dashboard Cite

show abstract

“…In this context, titanium dioxide (TiO 2 ) represents a gold standard in bone tissue engineering, in fact the nanotopography of TiO 2 raised several important properties, which allow the use of this scaffold to mimic the morphology and the hierarchical organization typical of the extra cellular matrix (ECMs) in bone [11,12]. TiO 2 nanostructured surface was obtained by the deposition of a supersonic beam of TiO x clusters.…”

Section: Introductionmentioning

confidence: 99%

Nanostructured TiO2 Surfaces Promote Human Bone Marrow Mesenchymal Stem Cells Differentiation to Osteoblasts

et al. 2016

View full text Add to dashboard Cite

Micro- and nano-patterning/modification are emerging strategies to improve surfaces properties that may influence critically cells adherence and differentiation. Aim of this work was to study the in vitro biological reactivity of human bone marrow mesenchymal stem cells (hBMSCs) to a nanostructured titanium dioxide (TiO2) surface in comparison to a coverglass (Glass) in two different culture conditions: with (osteogenic medium (OM)) and without (proliferative medium (PM)) osteogenic factors. To evaluate cell adhesion, hBMSCs phosphorylated focal adhesion kinase (pFAK) foci were analyzed by confocal laser scanning microscopy (CLSM) at 24 h: the TiO2 surface showed a higher number of pFAK foci with respect to Glass. The hBMSCs differentiation to osteoblasts was evaluated in both PM and OM culture conditions by enzyme-linked immunosorbent assay (ELISA), CLSM and real-time quantitative reverse transcription PCR (qRT-PCR) at 28 days. In comparison with Glass, TiO2 surface in combination with OM conditions increased the content of extracellular bone proteins, calcium deposition and alkaline phosphatase activity. The qRT-PCR analysis revealed, both in PM and OM, that TiO2 surface increased at seven and 28 days the expression of osteogenic genes. All together, these results demonstrate the capability of TiO2 nanostructured surface to promote hBMSCs osteoblast differentiation and its potentiality in biomedical applications.

show abstract

“…Intensive efforts have been made to determine the most adequate composition and architecture. On the chemical side, many materials varying in composition and architecture have been proposed, including polymers (Ishaug-Riley et al, 1997;Ignatius et al, 2001;Mondrinos et al, 2006;Gogolewski et al, 2008), metals (Ayers et al, 1999;Bobyn et al, 1999;Itala et al, 2001;Witte et al, 2007) and ceramics (Klawitter and Hulbert, 1971;Klein et al, 1985;van Blitterswijk et al, 1986;Eggli et al, 1988;Daculsi and Passuti, 1990;Schliephake et al, 1991;Basle et al, 1993;Metsger et al, 1993;Lu et al, 1999;Flautre et al, 2001;Walsh et al, 2003;Jones and Hench, 2004;Linhart et al, 2004;Hench, 2006;Von Doernberg et al, 2006;Lan Levengood et al, 2010;Murakami et al, 2010;Yuan et al, 2010;Polak et al, 2011;Haugen et al, 2013). These materials present very different resorption rates, and many resorption mechanisms, such as dissolution, hydrolysis (e.g., poly(α-hydroxy acids) (Ignatius et al, 2001)), cell-mediated resorption (Basle et al, 1993;Lu et al, 1999;Von Doernberg et al, 2006;Yuan et al, 2010), corrosion (Witte et al, 2007), enzymatic degradation (Hutmacher, 2000;Vert, 2007), and transport <...>…”

Section: Introductionmentioning

confidence: 99%

Effect of grain size and microporosity on the in vivo behaviour of β-tricalcium phosphate scaffolds

Lapczyna

Galea²,

Wüst

et al. 2014

eCM

View full text Add to dashboard Cite

Defining the most adequate architecture of a bone substitute scaffold is a topic that has received much attention over the last 40 years. However, contradictory results exist on the effect of grain size and microporosity. Therefore, the aim of this study was to determine the effect of these two factors on the in vivo behaviour of β-tricalcium phosphate (β-TCP) scaffolds. For that purpose, β-TCP scaffolds were produced with roughly the same macropore size (≈ 150 μm), and porosity (≈ 80 %), but two levels of microporosity (low: 10 % / high: ≈ 25 %) and grain size (small: 1.3 μm /large: ≈ 3.3 μm). The sample architecture was characterised extensively using materialography, Hg porosimetry, micro-computed tomography (μCT), and nitrogen adsorption. The scaffolds were implanted for 2, 4 and 8 weeks in a cylindrical 5-wall cancellous bone defect in sheep. The histological, histomorphometrical and μCT analysis of the samples revealed that all four scaffold types were almost completely resorbed within 8 weeks and replaced by new bone. Despite the three-fold difference in microporosity and grain size, very few biological differences were observed. The only significant effect at p < 0.01 was a slightly faster resorption rate and soft tissue formation between 4 and 8 weeks of implantation when microporosity was increased. Past and present results suggest that the biological response of this particular defect is not very sensitive towards physico-chemical differences of resorbable bone graft substitutes. As bone formed not only in the macropores but also in the micropores, a closer study at the microscopic and localised effects is necessary.

show abstract

Porous ceramic titanium dioxide scaffolds promote bone formation in rabbit peri-implant cortical defect model

Cited by 85 publications

References 65 publications

Scaffold Design for Bone Regeneration

Scaffold Design for Bone Regeneration

Nanostructured TiO2 Surfaces Promote Human Bone Marrow Mesenchymal Stem Cells Differentiation to Osteoblasts

Effect of grain size and microporosity on the in vivo behaviour of β-tricalcium phosphate scaffolds

Contact Info

Product

Resources

About