Porphyromonas gingivalis, a Long-Range Pathogen: Systemic Impact and Therapeutic Implications

Mulhall, Hannah; Huck, Olivier; Amar, Salomon

doi:10.3390/microorganisms8060869

Cited by 41 publications

(33 citation statements)

References 137 publications

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“…Nevertheless, the consequences of maintained elevated systemic CRP levels in women with BV are unknown given the lack of clinical evidence. On the other hand, PD is a recognized factor towards persistent inflammatory states on a plethora of other chronic diseases, for instance in diabetes, cardiovascular diseases or rheumatoid arthritis 17,[66][67][68] . Further, the treatment of gum disease is effective in alleviating this inflammatory burden [69][70][71] .…”

Section: Discussionmentioning

confidence: 99%

Association of bacterial vaginosis with periodontitis in a cross-sectional American nationwide survey

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Mendes

et al. 2021

Sci Rep

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To explore the association between bacterial vaginosis (BV) and periodontitis (PD) and to determine whether PD and BV might be linked with systemic serum alterations. We used the National Health and Nutrition Examination Survey 2001–2004, with women aged 18–49 years old and diagnosed with or without BV according to Nugent’s method. PD was defined according to the 2012 case definition. We compared serum counts according to the presence of PD and the presence of BV. Multivariable regression was used to explore and identify relevant variables towards the presence of BV. 961 women fulfilled the inclusion criteria. In women with BV, PD was associated with higher inflammation, characterized by increased white blood cells (p = 0.006) and lymphocyte (p = 0.009) counts. Predictive models presented a statistically significant association between PD and BV [Odds Ratio (OD) = 1.69, 95% Confidence Interval (CI): 1.09–2.61 for periodontitis; OD = 2.37, 95% CI: 1.30–4.29 for severe PD]. Fully adjusted models for age, smoking, body mass index, diabetes mellitus and number of systemic conditions reinforced this association [OD = 1.71, 95% CI: 1.06–2.76 for PD; OD = 2.21, 95% CI: 1.15–4.25 for severe PD]. An association between BV and PD is conceivable. PD was associated with higher systemic markers of inflammation in women with BV. Our data is novel and could serve as a foundation to guide future studies in the confirmation of this association and the underlying mechanisms.

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Section: Discussionmentioning

confidence: 99%

Association of bacterial vaginosis with periodontitis in a cross-sectional American nationwide survey

Escalda

Botelho

Mendes

et al. 2021

Sci Rep

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“…Deletion of this gene resulted in reduced biofilm formation and downregulated virulence factor gene expression 41 . In another study, the presence of the cas 3 gene was linked with an increase in the virulence of Porphyromonas gingivalis , 42 a keystone pathogen commonly associated with periodontitis as well as other clinical conditions, including Alzheimer's disease, 43 rheumatoid arthritis, 44 cardiovascular disease, 45 and pneumonia 46 . The P .…”

Section: Crispr–cas Can Be Utilized To Investigate Biological/genetic Attributes Of the Oral Biofilm In Search Of Novel Therapeuticsmentioning

confidence: 99%

The role of CRISPR‐Cas in advancing precision periodontics

Barbour

Glogauer

Grinfeld

et al. 2021

J of Periodontal Research

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The significant advancement of molecular biology has revolutionized medicine and provided important technologies to further clinical research development. Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) are DNA sequences derived from bacteriophages which have previously infected the bacterial species. The CRISPR–Cas system plays a key role in bacterial defense by detecting and destroying DNA fragments during subsequent bacteriophage invasions. The Cas9 enzyme recognizes and cleaves new invading CRISPR‐complementary DNA sequences. Researchers have taken advantage of this biological device to manipulate microbes’ genes and develop novel therapeutics to tackle systemic disease. In this review, we discuss the potential of utilizing CRISPR–Cas systems in the periodontal field to develop personalized periodontal care. We summarize promising attempts to bring this technology to the clinical setting. Finally, we provide insights regarding future developments to best utilize the CRISPR–Cas systems to advance precision periodontics. Although further research is imperative to evaluate the safety and potential of using CRISPR–Cas to develop precision periodontics approaches, few studies showed promising data to support the investment into this important technology in the dental sector. CRISPR–Cas9 can be a useful tool to create knockouts in vitro and in vivo as a screening tool to identify cellular pathways involved in the pathogenesis of periodontitis. Alternative CRISPR systems such as CRISPRa, CRISPRi, and Cas13 can be used to modify the transcriptome and gene expression of genes involved in periodontitis progression. CRISPR systems such as Cas3 can be used to target the periodontal biofilm and to develop new strategies to reduce or eliminate periodontal pathogens. Currently, the utility of CRISPR–Cas applications in clinical settings is limited. Through this review, we hope to foster further discussion in the periodontal research and clinical communities with respect to the potential clinical application of novel, CRISPR–Cas based, therapeutics for periodontitis.

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“…We visited their homes at 10:00 in the morning to minimize the effect of food intake. Details of the survey were described in our previous report [27,28,37,38].…”

Section: Subject and Settingmentioning

confidence: 99%

“…Oral diseases, especially periodontal disease, can be the main risk factor for several systemic diseases [22][23][24][25][26][27]. Periodontal bacteria and their surface lipopolysaccharides were the agents of systemic diseases [28]. Recent advances of research on systemic diseases and oral health showed that there exist protective host factors for systemic diseases in relation to periodontal diseases [29][30][31].…”

Section: Introductionmentioning

confidence: 99%

Oral Microbiome in Four Female Centenarians

et al. 2020

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The oral microbiome of healthy older adults has valuable information about a healthy microbiome. In this study, we collected and analyzed the oral microbiome of denture plaque and tongue coating samples from four female centenarians. After DNA extraction and purification, pyrosequencing of the V3–V4 hypervariable regions of the 16S rRNA was carried out. The bacterial taxonomy for each lead was assigned based on a search of the EzBioCloud 16S database. We obtained a total of 199,723 valid, quality-controlled reads for denture plaque and 210,750 reads for tongue coating. The reads were assigned 407 operational taxonomic units with a 97% identity cutoff. Twenty-nine species were detected in both denture plaque and tongue coatings from all subjects. Firmicutes was the most abundant phylum; the Streptococcus salivarius group was the most abundant species in both the denture plaque and tongue coatings; and the Fusobacterium nucleatum group was detected in all subjects. In the bacterial profile, species formed clusters composed of bacteria with a wide range of prevalence and abundance, not dependent on phyla; each cluster may have specific species that could be candidates for a core microbiome. Firmicutes and Veillonella were abundant phyla on both plaque and tongue coatings of centenarians.

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Porphyromonas gingivalis, a Long-Range Pathogen: Systemic Impact and Therapeutic Implications

Cited by 41 publications

References 137 publications

Association of bacterial vaginosis with periodontitis in a cross-sectional American nationwide survey

Association of bacterial vaginosis with periodontitis in a cross-sectional American nationwide survey

The role of CRISPR‐Cas in advancing precision periodontics

Oral Microbiome in Four Female Centenarians

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