Porphyromonas gingivalis induces exacerbated periodontal disease during pregnancy

Duan, Xuefeng; Hays, Aislinn; Zhou, Wei; Sileewa, Nawar; Upadhyayula, Satya; Wang, Huizhi; Liang, Shuang

doi:10.1016/j.micpath.2018.08.019

Cited by 8 publications

(10 citation statements)

References 50 publications

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“…A ligature induced periodontal disease animal model, in which periodontal disease was induced by molar ligation and P. gingivalis infection, was modified as described in our previous publication [50]. Our results validated the model by showing that ligation and P. gingivalis infection caused significantly higher alveolar bone loss in pregnant mice (Fig.…”

Section: Exacerbated Periodontal Conditions In Pregnant Micesupporting

confidence: 78%

“…Treg cells have manifested their critical roles in down-regulating the host immunity and inflammation [24][25][26][27]. Indeed, we found that after periodontal disease induction, IL-10 expression was lower in the pregnant mice than non-pregnant mice [50], which led us to further investigate Treg cell responses in the exacerbated periodontal disease during pregnancy. We tested the gingival mRNA expression of Treg-related molecules, including Foxp3, TGFβ, CTLA4, and CD28.…”

Section: Discussionmentioning

confidence: 91%

“…While the inflammation during pregnancy can be detrimental to pregnant women's health, negatively impact their quality of life, and may even cause adverse pregnancy outcomes, the mechanism of the more severe oral inflammation is illusive. We modified a widely applied ligature model and utilized it to understand the pathogenesis of the disease in pregnant mice, as described in our previous publication [50]. This model induced inflammation in a shorter time period, which enabled us to fit the experiment into the time frame of pregnancy.…”

Section: Discussionmentioning

confidence: 99%

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Down-regulated Treg cells in exacerbated periodontal disease during pregnancy

Hays

Duan

Zhu

et al. 2019

International Immunopharmacology

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Pregnancy is a special period marked with complicated changes in various immune responses. Although pregnant women are prone to developing gingival inflammation, its immunological mechanism remains to be clarified. In a modified ligature-induced periodontal disease murine model, pregnant mice developed more severe alveolar bone loss. Using this model, we investigated the Treg responses during exacerbated periodontal disease in pregnant mice. We tested Tregassociated molecules in gingival tissues by quantitative real-time PCR and found decreased gingival expression of Foxp3, TGFβ, CTLA-4, and CD28 in pregnant mice after periodontal disease induction. We further confirmed that lower number of Treg cells were present in the cervical lymph nodes of pregnant periodontitis mice. Treg cells from the cervical lymph nodes of ligated pregnant mice and non-pregnant mice were tested for their suppressive function in vitro. We manifested that Treg suppressive function was also down-regulated in the pregnant mice. Additionally, we demonstrated that more inflammatory Th17 cells were present in the cervical lymph nodes of ligated pregnant mice. Therefore, impaired Treg development and function, together with upregulated Th17 response, may contribute to the exacerbated periodontal disease during pregnancy.

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Section: Exacerbated Periodontal Conditions In Pregnant Micesupporting

confidence: 78%

Section: Discussionmentioning

confidence: 91%

Section: Discussionmentioning

confidence: 99%

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Down-regulated Treg cells in exacerbated periodontal disease during pregnancy

Hays

Duan

Zhu

et al. 2019

International Immunopharmacology

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“…Mediators of Inflammation destruction of periodontal tissues is observed [102], and an imbalance in the Th17/Treg cell ratio with aggravation of periodontitis during pregnancy occurs [127]. Periodontitis causes gynecological problems, ranging from difficulty in embryo implantation to preterm delivery and low birth weight.…”

Section: Pregnancy and Periodontitismentioning

confidence: 99%

Influence of Gestational Hormones on the Bacteria-Induced Cytokine Response in Periodontitis

Ortiz-Sánchez

Legorreta-Herrera²,

Rodrı́guez-Sosa³

2021

Mediators of Inflammation

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Periodontitis is an inflammatory disease that affects the supporting structures of teeth. The presence of a bacterial biofilm initiates a destructive inflammatory process orchestrated by various inflammatory mediators, most notably proinflammatory cytokines, which are upregulated in the gingival crevicular fluid, leading to the formation of periodontal pockets. This represents a well-characterized microbial change during the transition from periodontal health to periodontitis; interestingly, the gestational condition increases the risk and severity of periodontal disease. Although the influence of periodontitis on pregnancy has been extensively reviewed, the relationship between pregnancy and the development/evolution of periodontitis has been little studied compared to the effect of periodontitis on adverse pregnancy outcomes. This review is aimed at summarizing the findings on the pregnancy-proinflammatory cytokine relationship and discussing its possible involvement in the development of periodontitis. We address (1) an overview of periodontal disease, (2) the immune response and possible involvement of proinflammatory cytokines in the development of periodontitis, (3) how bone tissue remodelling takes place with an emphasis on the involvement of the inflammatory response and metalloproteinases during periodontitis, and (4) the influence of hormonal profile during pregnancy on the development of periodontitis. Finally, we believe this review may be helpful for designing immunotherapies based on the stage of pregnancy to control the severity and pathology of periodontal disease.

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“…The presence of vaginal plug, as indicative of successful copulation, was considered day 1 of pregnancy. 33 After pregnancy was confirmed, the animals were randomly allocated into the following groups (n = 8).…”

Section: Sample Population and Sample Size Calculationmentioning

confidence: 99%

A new mixed model of periodontitis‐induced preeclampsia: A pilot study

Mata

Nobre

Silva

et al. 2021

J of Periodontal Research

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Objectives/Background: Recent studies have shown that periodontal disease is strongly related to gestational complications such as preeclampsia (PE). PE is responsible for 42% of maternal deaths worldwide and kills approximately 76 000 women a year. In addition, children born under PE conditions are at increased risk of hospitalization due to metabolic disorders, epilepsy, and other complications. Numerous reviews and clinical studies on PE have been published, but the mechanisms underlying the relationship between periodontal disease and PE and the way periodontopathogens alter vascular response in pregnant women remain unclear.Methods: This study aims to verify whether periodontal disease induces PE by using the association of two periodontitis (PD) models: ligature and oral Porphyromonas gingivalis (P. gingivalis) W83 inoculation in Wistar rats. At gestational day 5, the ligature was placed on each mandibular first molar, which was followed by daily oral P. gingivalis inoculation for 15 days. At gestational day 19, urine was collected, and invasive arterial pressure was measured. The animals were euthanized, and plasma and tissues were collected.Results: After 15 days of the association of ligature and P. gingivalis inoculation, the animals presented the characteristic symptoms of PE: altered blood pressure, proteinuria, and change in litter size (number of pups) and pup weight when compared to the control group (p < .005). The PE animals also presented greater bone porosity, trabecular separation, and reduced bone volume in the hemimandibles, as well as altered inflammatory response. The level of cytokine IL-6 was higher in the PE group than in the control group (p < .005). Conclusion:The association of two PD models effectively induced PE. To our knowledge, this is the first study on the oral use of P. gingivalis for PE induction. Our results support the importance of PD as a possible cause for PE development, opening an | 727 MATA eT Al.

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Porphyromonas gingivalis induces exacerbated periodontal disease during pregnancy

Cited by 8 publications

References 50 publications

Down-regulated Treg cells in exacerbated periodontal disease during pregnancy

Down-regulated Treg cells in exacerbated periodontal disease during pregnancy

Influence of Gestational Hormones on the Bacteria-Induced Cytokine Response in Periodontitis

A new mixed model of periodontitis‐induced preeclampsia: A pilot study

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