Portable Dielectrophoresis Microfluidic Chip Integrated With Microscopic Platform for Water Blooms Monitoring

Meng, Jiyu; Li, Shanshan; Yu, Chengzhuang; Cheng, Jingmeng; Dai, Shijie; Wei, Chunyang; Li, Junwei

doi:10.1109/jmems.2021.3065328

“…Therefore, liquid-liquid microfluidic systems have made great progress in the fields of chemical reaction, liquid-liquid extraction, bioanalysis, crystallization process regulation, and the preparation of structural materials. In order to analyze the effects of operating conditions, fluid properties, and channel structure on multiphase flow, many useful studies have been carried out from the perspective of experiment [ 1 ].…”

Section: Introductionmentioning

confidence: 99%

[Retracted] Application of CFD Numerical Simulation Image Imaging Technology in the Study of Droplet Microfluidic Multiphase Flow Characteristics

Li

¹

,

Hu

²

2022

Contrast Media & Molecular Imaging

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In order to study the problem that the flow in the internal channel of the microfluidic chip is different from that of the macroscopic system by the FD numerical simulation image imaging technology. Taking the liquid-liquid extraction of microfluidic chip as the research object, this paper analyzes the theoretical basis, working principle, structural parameters, and the influence of working parameters on the fluid flow of liquid-liquid extraction. The results are as follows: when the inlet velocity of flowing liquid is 10^(−5) m/s, the diffusion efficiency can still be maintained at 95%; the double ψ-type aqueous phase showed laminar flow, the two-phase contact interface increased compared with the bottom flow rate, and the extraction efficiency increased to 98%; the extraction efficiency of double ψ type is higher than that of double Y-type: when the flow velocity ratio increases from v a q : v oil = 1 : 2 to v a q : v oil = 5 : 1 , the extraction efficiency increases to 99.8%; the experimental extraction efficiency is compared with the diffusion efficiency simulated by simulation. The diffusion efficiency of the cross type is 1.05 times that of the extraction efficiency, and that of the cylindrical type is 1.04 times that of the extraction efficiency. In this study, CFD is used to simulate the characteristics of droplet microfluidic multiphase flow, which enriches the theoretical method and research experience of liquid-liquid laminar flow.

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“…For example, based on CE (capillary electrophoresis), IEF (isoelectric focusing), ELISA (enzyme-linked immunosorbent assay), HPLC (high-performance liquid chromatography), IRMS (isotope ratio mass spectrometry), MRI (magnetic resonance imaging), NMR (nuclear magnetic resonance), NIR (light and electron microscopy for histology and image analysis, near-infrared) spectroscopy, GC (gas chromatography), LC (liquid chromatography [3]), or MS (mass spectrometry) approaches, there is commercial equipment developed for meat authentication. PCR (polymerase chain reaction [4,5]) method is a well-established targeted technology, including gel electrophoresis PCR [6], real-time fluorescence quantitative PCR [7], and digital PCR [8,9]. Among these PCR detection technologies, digital PCR is the latest method for deoxyribonucleic acid (DNA) amplification [10,11].…”

Section: Introductionmentioning

confidence: 99%

An integrated digital polymerase chain reaction chip for multiplexed meat adulteration detection

Yu

¹

,

Dai

²

,

Zhang

³

et al. 2023

Self Cite

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Meat adulteration detection is a common concern of consumers. Here, we proposed a multiplex digital polymerase chain reaction method and a low‐cost device for meat adulteration detection. Using a polydimethylsiloxane microfluidic device, polymerase chain reaction reagents could be pump‐free loaded into microchambers (40 × 40 chambers) automatically. Due to the independence of multiplex fluorescence channels, deoxyribonucleic acid templates extracted from different animal species could be distinguished by one test. In this paper, we designed primers and probes for four types of meat (beef, chicken, pork, and duck) and labeled each of the four fluorescent markers (hexachlorocyclohexane [HEX], 6‐carboxyfluorescein [FAM], X‐rhodamine [ROX], and cyanine dyes 5 [CY5]) on the probes. Specific detection and mixed detection experiments were performed on four types of meat, realizing a limit of detection of 3 copies/µL. A mixture of four different species can be detected by four independent fluorescence channels. The quantitative capability of this method is found to meet the requirements of meat adulteration detections. This method has great potential for point‐of‐care testing together with portable microscopy equipment.

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“…For example, mini-microscope systems built on polymethyl methacrylate (PMMA) architecture allow real-time monitoring of cell behavior in a thermostat [24]. Lensfree computational microscopes [25] have also proven to be suitable for low-cost portable architectures, using the lens-free imaging system for real-time imaging analysis of human malaria parasites [26].Based on our previous work on bright-field portable microscope prototypes [17,27] on the applications of dairy somatic cell counting and microalgae cell counting, here we have further developed a high-resolution fluorescence microscope system that is low-cost, portable and capable of adjustable mechanical movements. Compared with previous work, the proposed prototype has a compact XYZ stage to facilitate the operator to perform optical investigations in a larger area.…”

Section: Introductionmentioning

confidence: 99%

A Cost-Effective Nucleic Acid Detection System Using a Portable Microscopic Device

Yu

¹

,

Li

²

,

Wei

³

et al. 2022

Self Cite

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A fluorescence microscope is one of the most important tools for biomedical research and laboratory diagnosis. However, its high cost and bulky size hinder the application of laboratory microscopes in space-limited and low-resource applications. Here, in this work, we proposed a portable and cost-effective fluorescence microscope. Assembled from a set of 3D print components and a webcam, it consists of a three-degree-of-freedom sliding platform and a microscopic imaging system. The microscope is capable of bright-field and fluorescence imaging with micron-level resolution. The resolution and field of view of the microscope were evaluated. Compared with a laboratory-grade inverted fluorescence microscope, the portable microscope shows satisfactory performance, both in the bright-field and fluorescence mode. From the configurations of local resources, the microscope costs around USD 100 to assemble. To demonstrate the capability of the portable fluorescence microscope, we proposed a quantitative polymerase chain reaction experiment for meat product authenticating applications. The portable and low-cost microscope platform demonstrates the benefits in space-constrained environments and shows high potential in telemedicine, point-of-care testing, and more.

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Portable Dielectrophoresis Microfluidic Chip Integrated With Microscopic Platform for Water Blooms Monitoring

Cited by 5 publications

References 29 publications

[Retracted] Application of CFD Numerical Simulation Image Imaging Technology in the Study of Droplet Microfluidic Multiphase Flow Characteristics

[Retracted] Application of CFD Numerical Simulation Image Imaging Technology in the Study of Droplet Microfluidic Multiphase Flow Characteristics

An integrated digital polymerase chain reaction chip for multiplexed meat adulteration detection

A Cost-Effective Nucleic Acid Detection System Using a Portable Microscopic Device

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