Portable guided-mode resonance biosensor platform for point-of-care testing

Sung, Gun Yong; Kim, Wan‐Joong; Ko, Hyunsung; Kim, Bong Kyu; Kim, Kyung-Hyun; Huh, Chul; Hong, Jin-Woong

doi:10.1117/12.929457

Cited by 5 publications

(1 citation statement)

References 21 publications

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“…In several previous works the functionality of this principle was demonstrated by tracking the shift of the GMR wavelength Δλ during biomarker detection with a spectrometer [16,18]. For compact point-of-care systems miniaturization of the spectrometer setup [19] as well as a smartphone adapter and use of the smartphone camera [20] have been investigated. For multiplexed detection of a biomarker array, a compact spectrometer-free setup is preferable.…”

Section: Introductionmentioning

confidence: 99%

Handheld imaging photonic crystal biosensor for multiplexed, label-free protein detection

Jahns¹,

Brau²,

Meyer³

et al. 2015

Biomed. Opt. Express

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We present a handheld biosensor system for the label-free and specific multiplexed detection of several biomarkers employing a spectrometer-free imaging measurement system. A photonic crystal surface functionalized with multiple specific ligands forms the optical transducer. The photonic crystal slab is fabricated on a glass substrate by replicating a periodic grating master stamp with a period of 370 nm into a photoresist via nanoimprint lithography and deposition of a 70-nm titanium dioxide layer. Capture molecules are coupled covalently and drop-wise to the photonic crystal surface. With a simple camera and imaging optics the surface-normal transmission is detected. In the transmission spectrum guided-mode resonances are observed that shift due to protein binding. This shift is observed as an intensity change in the green color channel of the camera. Non-functionalized image sections are used for continuous elimination of background drift. In a first experiment we demonstrate the specific and time-resolved detection of 90.0 nm CD40 ligand antibody, 90.0 nM EGF antibody, and 500 nM streptavidin in parallel on one sensor chip. In a second experiment, aptamers with two different spacer lengths are used as receptor. The binding kinetics with association and dissociation of 250 nM thrombin and regeneration of the sensor surface with acidic tris-HCl-buffer (pH 5.0) is presented for two measurement cycles.

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Section: Introductionmentioning

confidence: 99%

Handheld imaging photonic crystal biosensor for multiplexed, label-free protein detection

Jahns¹,

Brau²,

Meyer³

et al. 2015

Biomed. Opt. Express

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show abstract

Integration of a guided-mode resonance filter with microposts for in-cell protein detection

Tsai

Lee

et al. 2016

Analyst

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We present an integrated microfluidic system consisting of a label-free biosensor of a guided-mode resonance filter (GMRF) and a microfluidic channel with a micropost filter. The GMRF was fabricated through replica molding using an ultraviolet-curable polymer and a plastic substrate. An array of microposts (a diameter and height of 26.5 and 56 μm, respectively, and a spacing between 7.5 and 9.5 μm), fabricated on a silicon substrate through photolithography, was used as the filter. A double-sided tape was used to laminate the GMRF and a microfluidic chip such that the integrated device provides two functions: filtration of the cell debris and quantification of the in-cell protein concentration. By measuring the changes in the resonant wavelength from the GMRF, the detection of β-actin in an unprocessed lysed cell sample was demonstrated; the cell debris was separated using the micropost filter to prevent false measurement.

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Real-time CRP detection from whole blood using micropost-embedded microfluidic chip incorporated with label-free biosensor

Tsai

Hsiung

Chen

et al. 2018

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We demonstrate the detection of C-creative protein (CRP) from whole blood samples without sample pretreatment by using a lab-on-a-chip system consisting of a microfluidic chip and a label-free biosensor. The microfluidic chip includes an array of microposts for filtering blood cells and allows only plasma to flow through and reach the guided-mode resonance (GMR) biosensor for real-time monitoring. The developed GMR sensor can achieve a bulk sensitivity of 186 nm RIU, which supports a limit of detection of 3.2 ng mL for recombinant CRP spiked in human serum. The results are comparable with those obtained using enzyme-linked immunosorbent assay. In addition, we demonstrate the efficacy of filtration of blood cells using microposts and simultaneous measurement of CRP concentration using a GMR sensor by using whole blood and plasma samples.

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Portable guided-mode resonance biosensor platform for point-of-care testing

Cited by 5 publications

References 21 publications

Handheld imaging photonic crystal biosensor for multiplexed, label-free protein detection

Handheld imaging photonic crystal biosensor for multiplexed, label-free protein detection

Integration of a guided-mode resonance filter with microposts for in-cell protein detection

Real-time CRP detection from whole blood using micropost-embedded microfluidic chip incorporated with label-free biosensor

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