Portable quantitative phase microscope for material metrology and biological imaging

Abstract

Quantitative phase microscopy (QPM) has emerged as an important tool for material metrology and biological imaging. For broader adoption in those applications, we have proposed and demonstrated a new portable off-axis QPM method, which works in both transmission and reflection modes to meet different sample measurement requirements. The temporal and spatial sensitivities of our system, as quantified by optical path-length difference values, are 0.65 nm and 1.04 nm, respectively. To demonstrate its applicability for a wide range of applications, we deployed our system for profiling transistor gold electrode samples, observing red blood cell membrane fluctuations, imaging living cells flowing in a microfluidic chip, etc. Our portable QPM system has a low-cost design and involves a simple and robust phase-retrieval algorithm that we envision will allow for broader deployment at different environmental settings, including in resource-limited sites and integration with other metrology or imaging modalities.

Multiparametric quantitative phase imaging for real-time, single cell, drug screening in breast cancer

“…S2a). We calibrated the phase measurements by imaging polystyrene microbeads (Polysciences, Warrington, PA), a common calibration standard for QPI microscopes [51][52][53][54] . To prepare our bead sample, we diluted the bead stock by a factor of 10 such that our bead solution was ~0.25% microbeads (weight/volume).…”

Artificial Intelligence Enabled Reagent-free Imaging Hematology Analyzer

“…The combined QPM-Raman system obviates the need of any additional separation method to select B lymphocytes either from the blood or from the leucocyte mixtures for leukemia diagnosis in a label-free manner. Moreover, as AIRFIHA involves a low-cost system that requires minimal sample preparation or chemical consumables, our AIRFIHA has a great potential to be used in point-of-care applications, resource-limited settings, or pandemic situations, e.g., COVID-19 pandemic, in view of a portable and low-cost QPM system recently demonstrated by us (Niu et al, 2020).…”

Artificial‐Intelligence‐Enabled Reagent‐Free Imaging Hematology Analyzer

“…The combined QPM-Raman system obviates the need of any additional separation method to select B lymphocytes either from the blood or from the leucocyte mixtures for leukemia diagnosis in a label-free manner. Moreover, as AIRFIHA involves a low-cost system that requires minimal sample preparation or chemical consumables, our AIRFIHA has a great potential to be used in point-of-care applications, resource-limited settings, or pandemic situations, e.g., COVID-19 pandemic, in view of a portable and low-cost QPM system recently demonstrated by Niu et al [71] 4. Experimental Section…”