Poseidonibacter parvus sp. nov., isolated from a squid

Abstract

A Gram-stain-negative, aerobic, short rod-shaped, motile, brownish-coloured bacterium, termed strain LPB0137T, was isolated from a squid. Its cells could grow weakly on marine agar 2216 with 0.04 % 2,3,5-triphenyl tetrazolium chloride (TTC). Each cell of strain LPB0137T has a circular chromosome with a length of 2.87 Mb and 27.7 mol% DNA G+C content. The genome includes 2698 protein-coding genes and six rRNA operons. In 16S rRNA gene sequence trees, strain LPB0137T formed a robust monophyletic clade with Poseidonibacter antarcticus SM1702T with a sequence similarity of 98.3 %. However, the average nucleotide identity and in silico DNA–DNA hybridization values between the two type strains were low (83.9 and 28.1 %, respectively). The overall phenotypic and genomic features of strain LPB0137T supported its assignment to the genus Poseidonibacter . However, the relatively low gene and genome sequence similarity between this strain and other type strains of the genus Poseidonibacter and several enzymatic characteristics indicated the taxonomic novelty of the isolated strain as a new member of the genus Poseidonibacter . Therefore, based on the phylogenetic and phenotypic characteristics of LPB0137T, we proposed a novel species of the genus Poseidonibacter for it, with the name Poseidonibacter parvus sp. nov. The type strain of this new species is thus LPB0137T (=KACC 18888T=JCM 31548T).

Aliarcobacter, Halarcobacter, Malaciobacter, Pseudarcobacter and Poseidonibacter are later synonyms of Arcobacter: transfer of Poseidonibacter parvus, Poseidonibacter antarcticus, ‘Halarcobacter arenosus’, and ‘Aliarcobacter vitoriensis’ to Arcobacter as Arcobacter parvus comb. nov., Arcobacter antarcticus comb. nov., Arcobacter arenosus comb. nov. and Arcobacter vitoriensis comb. nov.

“…lacus ) and one of Halarcobacter ( ‘H. arenosus ’) have been proposed [9–12]. Since the latter descriptions were not accounted for in our earlier extensive analyses, we re-examined their position relative to other Epsilonproteobacteria (including the Arcobacter clade) using phylogenomic and OGRI approaches as before [2].…”

“…antarcticus , ‘ Al. vitoriensis ’ and ‘ H. arenosus ’ [9–11], possesses a core phenotype (aerotolerance and psychrotrophism), enabling their ready distinction from all other Epsilonproteobacterial genera. In brief, all of our phenotypic, genomic and phylogenetic analyses [2] failed to establish appropriate genus-level distinction between the proposed novel genera of Pérez-Cataluña et al .…”

“…Basonym: Poseidonibacter parvus Kim et al . 2021 [11]. The description is identical to that given by [11].…”

“…2021 [11]. The description is identical to that given by [11]. The type strain is LPB0137 T (=KACC 18888 T =JCM 31548 T ).…”

“…vitoriensis' and 'H. arenosus' [9][10][11], possesses a core phenotype (aerotolerance and psychrotrophism), enabling their ready distinction from all other Epsilonproteobacterial genera. In brief, all of our phenotypic, genomic and phylogenetic analyses [2] failed to establish appropriate genus-level distinction between the proposed novel genera of Pérez-Cataluña et al [5] when placed into context within the wider Epsilonproteobacteria, but emphasised the relative homogeneity and distinct phylogenetic position Arcobacter sensu lato has in that same context.…”