Positive and Negative Haematopoietic Cytokines Produced by Bone Marrow Endothelial Cells

Li, Wei Min; Huang, Wei; Huang, Yan; Jiang, De Zhao; Wang, Qi Ru

doi:10.1006/cyto.1999.0678

Cited by 47 publications

(31 citation statements)

References 39 publications

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“…The intracellular concentration of AcSDKP increases at the beginning of culture and decreases progressively as the cells approach confluence. These data are consistent with the recently reported presence of AcSDKP in extracts of bone marrow endothelial cells 39,40 and suggest that AcSDKP produced constitutively by the endothelium may act in an autocrine manner to induce angiogenesis. Here we report that AcSDKP indeed acts in vitro as a positive regulator of endothelial cell motility and differentiation, 2 essential components for new vessel formation.…”

Section: Discussionsupporting

confidence: 92%

The tetrapeptide AcSDKP, an inhibitor of primitive hematopoietic cell proliferation, induces angiogenesis in vitro and in vivo

Liu

Lawrence

Kovacevic

et al. 2003

Blood

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The tetrapeptide acetyl-Ser-Asp-Lys-Pro (AcSDKP), purified from bone marrow and constitutively synthesized in vivo, belongs to the family of negative regulators of hematopoiesis. It protects the stem cell compartment from the toxicity of anticancer drugs and irradiation and consequently contributes to a reduction in marrow failure. This current work provides experimental evidence for another novel biologic function of AcSDKP. We report that AcSDKP is a mediator of angiogen-

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Section: Discussionsupporting

confidence: 92%

The tetrapeptide AcSDKP, an inhibitor of primitive hematopoietic cell proliferation, induces angiogenesis in vitro and in vivo

Liu

Lawrence

Kovacevic

et al. 2003

Blood

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“…Intracellular thymosin ␤-4 is thought to be a G-actin sequestering molecule [13], while when secreted [14], especially as the thymosin ␤-4 sulfoxide, the molecule appears to act as a signaling molecule functioning in a number of ways including wound healing [11], inflammation [12], and hematopoesis [15]. Previous studies on cell lines have shown that thymosin ␤-4 is overexpressed on the mRNA and protein level in various cell lines [16].…”

Section: Resultsmentioning

confidence: 99%

Use of ProteinChip™ array surface enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF MS) to identify thymosin β-4, a differentially secreted protein from lymphoblastoid cell lines

Diamond¹,

Zhang

Gaiger

et al. 2003

J. Am. Soc. Mass Spectrom.

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The identification of proteins differentially expressed between cancer and normal cells is vital for the development of cancer diagnostics, therapeutics and vaccines. Using a ProteinChip Biomarker System (Ciphergen Biosystems, Fremont, CA) which combines ProteinChip™ technology with time-of-flight mass spectrometry, we have developed a simple method to screen and identify differentially secreted proteins from tumor cell lines. Mass spectra of the range of proteins secreted from normal B-cells were generated along with those secreted from Epstein-Barr virus transformed B-cells. A mass peak at m/z ϭ 4972.1 that was highly over-represented in the transformed B-cell line was chosen for identification and purified by reversed phase chromatography with concomitant monitoring of fractions by SELDI-TOF MS. The resulting purified protein was digested with trypsin and the peptide masses derived from the SELDI-TOF spectrum were used to search the public databases for protein identification. Fragment matching of the resulting peptides identified the protein as thymosin ␤-4. Using LC-electrospray ionization MS/MS, the identity of this protein was confirmed. Thymosin ␤-4 is a known marker in LCLs establishing the utility of this method to discover and identify proteins differentially expressed between cancers and their matched normal counterparts. (J Am Soc Mass Spectrom 2003, 14, 760 -765)

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“…23,24 One CRU represents the repopulating ability shown by a standard number of fresh competitor cells (150 000 adult low-density BM cells in present experiments). To calculate the number of CRUs present in an unknown donor HSC sample, the percentage of donor type cells in the peripheral blood of the recipient called P d and the number of competitor cells ( ϫ 10 4 ) termed C are used to solve the equation: CRU ϭ P d ϫ C/(100ϪP d ).…”

Section: Transplantationmentioning

confidence: 99%

Primary endothelial cells isolated from the yolk sac and para-aortic splanchnopleura support the expansion of adult marrow stem cells in vitro

Johnson

Shelley

et al. 2003

Blood

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The embryonic origin and development of hematopoietic and endothelial cells is highly interdependent. We hypothesized that primary endothelial cells from murine yolk sac and para-aortic splanchnopleura (P-Sp) may possess the capacity to expand hematopoietic stem cells (HSCs) and progenitor cells ex vivo. Using Tie2-GFP transgenic mice in combination with fluorochrome-conjugated monoclonal antibodies to vascular endothelial growth factor receptor-2 (Flk1) and CD41, we have successfully isolated pure populations of primary endothelial cells from 9.5-days after coitus (dpc) yolk sac and P-Sp. Adult murine bone marrow Sca-1 ؉ cKit ؉ lin ؊ cells were cocultured with yolk sac or P-Sp Tie2-GFP ؉ Flk-1 ؉ CD41 ؊ endothelial cell monolayers for 7 days and the total number of nonadherent cells increased 47-and 295-fold, respectively, and hematopoietic progenitor counts increased 9.4-and 11.4-fold, respectively. Both the yolk sac and P-Sp endothelial cell cocultures facilitated long-term (> 6 months) HSC competitive repopulating ability (2.8-to 9.8-fold increases, respectively). These data suggest that 9.5-dpc yolk sac-and P-Sp-derived primary Tie2-

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Positive and Negative Haematopoietic Cytokines Produced by Bone Marrow Endothelial Cells

Cited by 47 publications

References 39 publications

The tetrapeptide AcSDKP, an inhibitor of primitive hematopoietic cell proliferation, induces angiogenesis in vitro and in vivo

The tetrapeptide AcSDKP, an inhibitor of primitive hematopoietic cell proliferation, induces angiogenesis in vitro and in vivo

Use of ProteinChip™ array surface enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF MS) to identify thymosin β-4, a differentially secreted protein from lymphoblastoid cell lines

Primary endothelial cells isolated from the yolk sac and para-aortic splanchnopleura support the expansion of adult marrow stem cells in vitro

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