Possible functional scaffolds for periodontal regeneration

Shimauchi, Hidetoshi; Nemoto, Eiji; Ishihata, Hiroshi; Shimomura, Masatsugu

doi:10.1016/j.jdsr.2013.05.001

Cited by 46 publications

(56 citation statements)

References 92 publications

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“…Therefore, currently employed reabsorbable materials in periodontal therapy (e.g. nano/micro-sized calcium phosphate, hydroxyapatite or beta-tricalcium phosphate) may be disadvantageous, as dissolution behaviors are not always as long-lasting as required, and degradation products may not be completely cytocompatible [5,6]. The non-controllable calcium release of these materials may lead to inhibition, stimulation or no effect on osteoconductivity, osteoclastic activity or even fibrous formation at the implanted tissue.…”

Section: Introductionmentioning

confidence: 99%

“…The non-controllable calcium release of these materials may lead to inhibition, stimulation or no effect on osteoconductivity, osteoclastic activity or even fibrous formation at the implanted tissue. The attained effect will depend on the variations of extracellular calcium concentration values [5,6], and it is not predictable with reabsorbable materials.…”

Section: Introductionmentioning

confidence: 99%

“…COO - ) are along the backbone of the present polymeric NPs, and these functional groups facilitate the possibility of calcium and zinc quelation. Calcium ions have been previously employed to increase extracellular calcium concentration, because it is a potent chemical signal to produce cell migration, cell growth and for the initiation of bone remodeling [5]. Improved osteogenic ability [11] and increased osteoconductivity [12] have been associated with the use of calcium containing materials.…”

Section: Introductionmentioning

confidence: 99%

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Bioactive Polymeric Nanoparticles for Periodontal Therapy

et al. 2016

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Aimsto design calcium and zinc-loaded bioactive and cytocompatible nanoparticles for the treatment of periodontal disease.MethodsPolymP-nActive nanoparticles were zinc or calcium loaded. Biomimetic calcium phosphate precipitation on polymeric particles was assessed after 7 days immersion in simulated body fluid, by scanning electron microscopy attached to an energy dispersive analysis system. Amorphous mineral deposition was probed by X-ray diffraction. Cell viability analysis was performed using oral mucosa fibroblasts by: 1) quantifying the liberated deoxyribonucleic acid from dead cells, 2) detecting the amount of lactate dehydrogenase enzyme released by cells with damaged membranes, and 3) by examining the cytoplasmic esterase function and cell membranes integrity with a fluorescence-based method using the Live/Dead commercial kit. Data were analyzed by Kruskal-Wallis and Mann-Whitney tests.ResultsPrecipitation of calcium and phosphate on the nanoparticles surfaces was observed in calcium-loaded nanoparticles. Non-loaded nanoparticles were found to be non-toxic in all the assays, calcium and zinc-loaded particles presented a dose dependent but very low cytotoxic effect.ConclusionsThe ability of calcium-loaded nanoparticles to promote precipitation of calcium phosphate deposits, together with their observed non-toxicity may offer new strategies for periodontal disease treatment.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Bioactive Polymeric Nanoparticles for Periodontal Therapy

et al. 2016

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“…This has been met with limited success using biologically active agents and guided tissue regenerative (GTR) or guided bone regeneration (GBR) membranes [1,2]. The ideal requirements for a GTR membrane include; a cell isolating occlusive biomaterial which meets minimum mechanical, physical, structural and biocompatibility requirements; ability to support organized and vascularized ingrowth and wound stabilization; protecting the underlying blood clot and thereby limiting the epithelial and unwanted connective tissue growth into the defect; promoting functional tissue regeneration from the relevant cells in the defect (avoiding healing by repair); and degrading in adequate time to provide space for newly formed periodontal tissue.…”

Section: Introductionmentioning

confidence: 99%

Freeze gelated porous membranes for periodontal tissue regeneration

et al. 2015

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Guided tissue regeneration (GTR) membranes have been used for the management of destructive forms of periodontal disease as a means of aiding regeneration of lost supporting tissues, including the alveolar bone, cementum, gingiva and periodontal ligaments (PDL). Currently available GTR membranes are either non-biodegradable, requiring a second surgery for removal, or biodegradable. The mechanical and biofunctional limitations of currently available membranes result in a limited and unpredictable treatment outcome in terms of periodontal tissue regeneration. In this study, porous membranes of chitosan (CH) were fabricated with or without hydroxyapatite (HA) using the simple technique of freeze gelation (FG) via two different solvents systems, acetic acid (ACa) or ascorbic acid (ASa). The aim was to prepare porous membranes to be used for GTR to improve periodontal regeneration. FG membranes were characterized for ultra-structural morphology, physiochemical properties, water uptake, degradation, mechanical properties, and biocompatibility with mature and progenitor osteogenic cells. Fourier transform infrared (FTIR) spectroscopy confirmed the presence of hydroxyapatite and its interaction with chitosan. μCT analysis showed membranes had 85-77% porosity. Mechanical properties and degradation rate were affected by solvent type and the presence of hydroxyapatite. Culture of human osteosarcoma cells (MG63) and human embryonic stem cell-derived mesenchymal progenitors (hES-MPs) showed that all membranes supported cell proliferation and long term matrix deposition was supported by HA incorporated membranes. These CH and HA composite membranes show their potential use for GTR applications in periodontal lesions and in addition FG membranes could be further tuned to achieve characteristics desirable of a GTR membrane for periodontal regeneration.

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“…The desire to induce the complete regeneration of periodontal tissue has inspired the introduction of Guided Tissue Regeneration technique. 4 The term "Guided Tissue Regeneration (GTR)" was given by Gottlow in 1986. The 1996 World Workshop in Periodontics defined GTR as "procedures attempting to regenerate lost periodontal structures through differential tissue responses".…”

Section: Introductionmentioning

confidence: 99%

Electrospinned bioactive glass/nano hydroxyapatite reinforced polycaprolactone GTR/GBR membranes in periodontics- A review

2020

IJPI

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Periodontitis, a chronic inflammatory infectious disease triggered by an interaction between microbial biofilm and host response leading to gingival bleeding, connective tissue destruction , alveolar bone loss and finally causing tooth loss. Periodontal regeneration or restoration is the ultimate goal of any periodontal treatment. This can be achieved by GTR/GBR [Guided Tissue Regeneration/Guided Bone Regeneration] membranes along with bone replacement grafts. With the evolution of Tissue engineering and different generation of GTR/GBR nanofibrous membranes, periodontal restoration or regeneration can be easily and rapidly achieved . Nanofibrous GTR/GBR Membranes can be fabricated using natural or synthetic polymers by different techniques such as Phase separation, Self assembly and Electrospinning [E-spinning]. Of these, E-spinning is the most widely studied technique and also seems to exhibit the most promising results for Tissue Engineering [TE] applications . This review addresses the Electrospinning method of GTR/GBR membrane fabrication using Bioactive Glass [BG]/Nano Hydroxyapatite[nHAP] reinforced polycaprolactone polymer and its advantages and disadvantages.

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Possible functional scaffolds for periodontal regeneration

Cited by 46 publications

References 92 publications

Bioactive Polymeric Nanoparticles for Periodontal Therapy

Bioactive Polymeric Nanoparticles for Periodontal Therapy

Freeze gelated porous membranes for periodontal tissue regeneration

Electrospinned bioactive glass/nano hydroxyapatite reinforced polycaprolactone GTR/GBR membranes in periodontics- A review

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