Possible involvement of heterotrimeric G-protein signaling in Al-induced secretion of organic acid anions in Arabidopsis and rye

Li, Yao Yang; Tang, Xin; Yang, Lei; Yu, Yong; Li, Xiao Feng

doi:10.1007/s11104-014-2325-0

Cited by 7 publications

(7 citation statements)

References 37 publications

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“…IP 3 then binds to its receptor and initiates intracellular calcium (as Ca 2+ ) release from the ER. In agreement with previous findings in Arabidopsis and rye 9 , Al 3+ -enhancement of citrate secretion from stylo roots was significantly reduced by antagonists of PLC, IP 3 receptor and DAG, whereas treatment with TSG, a specific activator of PLC, resulted in an increase in citrate release (Fig. S4 ).…”

Section: Discussionsupporting

confidence: 92%

“…Gα signaling is terminated by the intrinsic GTPase activity of Gα, in which ras-related small GTPase superfamily function as switch molecules by cycling between GTP-bound activated state to a GDP-bound inactivated state 19 , 20 . In consistence with our previous data in Arabidopsis and rye 9 , the involvement of G-proteins in Al 3+ enhancement of citrate excretion in stylo roots was supported by pharmacological analysis and enhancement of GTPase activity revealed in this work. Importantly, our recent data demonstrated Al 3+ -induced secretion of oxalate in buckwheat and exudation of malate in wheat without elevation of GTPase activity in the roots of both species (unpublished data).…”

Section: Discussionsupporting

confidence: 92%

“…Our early investigation detected the activation of GTPase in response to Al 3+ in root plasma membrane of Arabidopsis and rye 9 . To test the role of heterotrimeric G-proteins in Al 3+ -induced secretion of citrate in stylo roots, presence of the proteins in the plasma membrane of R2 roots was evaluated (Fig.…”

Section: Resultsmentioning

confidence: 99%

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Insights into aluminum-tolerance pathways in Stylosanthes as revealed by RNA-Seq analysis

Jiang

Liu

et al. 2018

Sci Rep

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Stylo has a great potential for Al3+ resistance in acidic soils through secretion of citrate from the roots. To get insight into the molecular mechanisms responsible, transcriptomic changes were investigated in the roots after treatment with T01 (−Al3+, pH6.0), T02 (−Al3+, pH4.3) and T03 (50 µM AlCl3, pH4.3). In total, 83,197 unigenes generated from 130,933 contigs were obtained. Of them, 282, 148 and 816 differentially expressed unigenes (DEGs) were revealed in T01_vs_T02, T02_vs_T03 and T01_vs_T03 comparison, respectively (FDR < 0.001, log2FC > 2). DEGs by Al3+ were related to G-proteins, diacyglycerol and inositol metabolism, calcium-signaling, transcription regulation, protein modification and transporters for detoxification of Al3+. Additionally, Al3+ facilitates citrate synthesis via modifying gene expression of pathways responsible for citrate metabolism. Overall, Al3+ resistance in stylo involves multiple strategies and enhancement of citrate anabolism. The Al3+ signal transmits through heterotrimeric G-proteins, phospholipase C, inositol triphosphate, diacylglycerol, Ca2+ and protein kinases, thereby activating transcription and anion channels in plasma membrane, and resulting in citrate secretion from stylo roots.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionsupporting

confidence: 92%

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Insights into aluminum-tolerance pathways in Stylosanthes as revealed by RNA-Seq analysis

Jiang

Liu

et al. 2018

Sci Rep

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“…Recently, Li et al . 4 further reported that heterotrimeric G-protein-coordinated transduction of Al-stress signals may be associated with the secretion of OAs from roots of Arabidopsis and rye. However, studies detailing the signal regulation mechanism of Al-induced organic acid efflux are still lacking 5 .…”

Section: Introductionmentioning

confidence: 96%

MAPK-mediated auxin signal transduction pathways regulate the malic acid secretion under aluminum stress in wheat (Triticum aestivum L.)

Lin

Liu

et al. 2017

Sci Rep

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An isobaric tags for relative and absolute quantitative (iTRAQ)-based quantitative proteomic approach was used to screen the differentially expressed proteins during control treatment (CK), aluminum (Al) and Al+ indole-3-acetic acid (IAA) treatment of wheat lines ET8 (Al-tolerant). Further, the the expression levels of auxin response factor (ARF), Aux/IAA, Mitogen activated protein kinase (MAPK) 2c, and MAPK1a were analyzed. Results showed that 16 proteins were determined to be differentially expressed in response to Al and IAA co-treatment compared with Al alone. Among them, MAPK2c and MAPK1a proteins displayed markedly differential expression during the processes. The expression of ARF2 was upregulated and Aux/IAA was downregulated by Al, while both in concentration- and time-dependent manners. Western-blot detection of MAPK2c and MAPK1a indicated that Al upregulated MAPK2c and downregulated MAPK1a in both concentration- and time-dependent manners. Exogenous IAA could promote the expression of MAPK2c, but inhibit the expression of MAPK1a in the presence/absence of Al. These findings indicated that IAA acted as one of the key signaling molecule controls the response mechanism of wheat malic acid efflux to Al stress through the suppression/activation of Aux/IAA and ARFs, and the activity of MAPK2c and MAPK1a were positively or negatively regulated.

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“…Hence, the PM proteomic changes under Al stress in TBS roots were detected by a combination of TMT labeling and HPLC-MS/MS analysis in this study. In addition, PM proteins play important roles in Al signal transmembrane were presented (Li et al, 2015). The identified Al-induced changes of PM proteins lay a foundation for further study of the transmembrane mechanism of aluminum signaling related to citric acid secretion from TBS roots.…”

Section: Introductionmentioning

confidence: 99%

Plasma membrane proteomic analysis by TMT-PRM provides insight into mechanisms of aluminum resistance in tamba black soybean roots tips

Wei¹,

Jiang²,

Han³

et al. 2020

PeerJ

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Aluminum (Al) toxicity in acid soil is a worldwide agricultural problem that inhibits crop growth and productivity. However, the signal pathways associated with Al tolerance in plants remain largely unclear. In this study, tandem mass tag (TMT)-based quantitative proteomic methods were used to identify the differentially expressed plasma membrane (PM) proteins in Tamba black soybean (TBS) root tips under Al stress. Data are available via ProteomeXchange with identifier PXD017160. In addition, parallel reaction monitoring (PRM) was used to verify the protein quantitative data. The results showed that 907 PM proteins were identified in Al-treated plants. Among them, compared to untreated plants, 90 proteins were differentially expressed (DEPs) with 46 up-regulated and 44 down-regulated (fold change > 1.3 or < 0.77, p < 0.05). Functional enrichment based on GO, KEGG and protein domain revealed that the DEPs were associated with membrane trafficking and transporters, modifying cell wall composition, defense response and signal transduction. In conclusion, our results highlight the involvement of GmMATE13, GmMATE75, GmMATE87 and H+-ATPase in Al-induced citrate secretion in PM of TBS roots, and ABC transporters and Ca2+ have been implicated in internal detoxification and signaling of Al, respectively. Importantly, our data provides six receptor-like protein kinases (RLKs) as candidate proteins for further investigating Al signal transmembrane mechanisms.

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Possible involvement of heterotrimeric G-protein signaling in Al-induced secretion of organic acid anions in Arabidopsis and rye

Cited by 7 publications

References 37 publications

Insights into aluminum-tolerance pathways in Stylosanthes as revealed by RNA-Seq analysis

Insights into aluminum-tolerance pathways in Stylosanthes as revealed by RNA-Seq analysis

MAPK-mediated auxin signal transduction pathways regulate the malic acid secretion under aluminum stress in wheat (Triticum aestivum L.)

Plasma membrane proteomic analysis by TMT-PRM provides insight into mechanisms of aluminum resistance in tamba black soybean roots tips

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