Possible involvement of phosphatidylinositol 3‐kinase in the maintenance of metaphase II attest in porcine oocytes matured <i>in vitro</i>

Ito, Junya; Shimada, M.; Kashiwazaki, Naomi

doi:10.1111/j.1740-0929.2009.00717.x

Cited by 4 publications

(2 citation statements)

References 36 publications

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“…Western blotting was carried out as previously reported (Ito et al . ). GV, MII, exposed MII and vitrified‐warmed MII oocytes were collected as described above.…”

Section: Methodsmentioning

confidence: 97%

Vitrification procedure decreases inositol 1,4,5‐trisphophate receptor expression, resulting in low fertility of pig oocytes

Hirose

Kamoshita

Fujiwara

et al. 2013

Animal Science Journal

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Although cryopreservation of mammalian oocytes is an important technology, it is well known that unfertilized oocytes, especially in pigs, are highly sensitive to low temperature and that cryopreserved oocytes show low fertility and developmental ability. The aim of the present study was to clarify why porcine in vitro matured (IVM) oocytes at the metaphase II (MII) stage showed low fertility and developmental ability after vitrification. In vitro matured cumulus oocyte complexes (COCs) were vitrified with Cryotop and then evaluated for fertility through in vitro fertilization (IVF). Although sperm-penetrated oocytes were observed to some extent (30-40%), the rate of pronuclear formation was low (9%) and none of them progressed to the two-cell stage. The results suggest that activation ability of cryopreserved oocytes was decreased by vitrification. We examined the localization and expression level of the type 1 inositol 1,4,5 trisphosphate receptor (IP3 R1), the channel responsible for Ca(2+) release during IVF in porcine oocytes. Localization of IP3 R1 close to the plasma membrane and total expression level of IP3 R1 protein were both decreased by vitrification. In conclusion, our present study indicates that vitrified-warmed porcine COCs showed a high survival rate but low fertility after IVF. This low fertility seems to be due to the decrease in IP3 R1 by the vitrification procedure.

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“…Western blotting was carried out as previously reported (Ito et al . ). GV, MII, exposed MII and vitrified‐warmed MII oocytes were collected as described above.…”

Section: Methodsmentioning

confidence: 97%

Vitrification procedure decreases inositol 1,4,5‐trisphophate receptor expression, resulting in low fertility of pig oocytes

Hirose

Kamoshita

Fujiwara

et al. 2013

Animal Science Journal

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“…1a). Possible involvement of phosphatidylinositol‐3 kinase on the maintenance of MAPK activity has been reported (Ito et al . 2010a).…”

Section: Meiotic Arrest At the MII Stage And Release From The Arrestmentioning

confidence: 95%

Molecular mechanism of fertilization in the pig

Ito

Kashiwazaki

2012

Animal Science Journal

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At fertilization, the sperm triggers resumption from the arrest, extrusion of the second polar body and pronuclear formation, the events of which are collectively acknowledged as 'oocyte activation'. In all species up to date, oocyte activation requires a fertilization-associated increase in the intracellular concentration of calcium. Especially in mammals, the signal of intracellular calcium rise at fertilization consists of periodical rises, which are also referred to as calcium oscillations. Our recent results suggest that these calcium oscillations have an important role in not only oocyte activation but also development of mammals. Pigs are animals of great agricultural value and ones in which assisted reproductive techniques, including somatic cell nuclear transfer, to produce gene-modified pigs. Although reconstructed embryos require artificial activation stimuli which mimic fertilization-associated increase of intracellular calcium in the oocytes, it has been known that the developmental ability of the oocytes after artificial activation is low and the regimen seems to be required for improvement. Recently we focused on two molecules, phospholipase C zeta and inositol 1,4,5-triphosphate receptor which have important roles in regulation of calcium oscillations during fertilization in mammals, including pigs. In this review, we will discuss the present status and future perspective of molecular mechanisms during fertilization in pigs.

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Possible involvement of Class III phosphatidylinositol-3-kinase in meiotic progression of porcine oocytes beyond germinal vesicle stage

et al. 2011

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Possible involvement of phosphatidylinositol 3‐kinase in the maintenance of metaphase II attest in porcine oocytes matured in vitro

Cited by 4 publications

References 36 publications

Vitrification procedure decreases inositol 1,4,5‐trisphophate receptor expression, resulting in low fertility of pig oocytes

Vitrification procedure decreases inositol 1,4,5‐trisphophate receptor expression, resulting in low fertility of pig oocytes

Molecular mechanism of fertilization in the pig

Possible involvement of Class III phosphatidylinositol-3-kinase in meiotic progression of porcine oocytes beyond germinal vesicle stage

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