Possible modulation of FAS and PTP-1B signaling in ameliorative potential of Bombax ceiba against high fat diet induced obesity

Abstract

BackgroundBombax ceiba Linn., commonly called as Semal, is used in various gastro-intestinal disturbances. It contains Lupeol which inhibits PTP-1B, adipogenesis, TG synthesis and accumulation of lipids in adipocytes and adipokines whereas the flavonoids isolated from B. ceiba has FAS inhibitory activity. The present study was aimed to investigate ameliorative potential of Bombax ceiba to experimental obesity in Wistar rats, and its possible mechanism of action.MethodsMale Wistar albino rats weighing 180-220 g were employed in present study. Experimental obesity was induced by feeding high fat diet for 10 weeks. Methanolic extract of B. ceiba extract 100, 200 and 400 mg/kg and Gemfibrozil 50 mg/kg as standard drug were given orally from 7th to 10th week.ResultsInduction with HFD for 10 weeks caused significant (p < 0.05) increase in % body wt, BMI, LEE indices; serum glucose, triglyceride, LDL, VLDL, cholesterol, free fatty acid, ALT, AST; tissue TBARS, nitrate/nitrite levels; different fat pads and relative liver weight; and significant decrease in food intake (g and kcal), serum HDL and tissue glutathione levels in HFD control rats. Treatment with B. ceiba extract and Gemfibrozil significantly attenuated these HFD induced changes, as compared to HFD control. The effect of B. ceiba 200 and 400 mg/kg was more pronounced in comparison to Gemfibrozil.ConclusionOn the basis of results obtained, it may be concluded that the methanolic extract of stem bark of Bombax ceiba has significant ameliorative potential against HFD induced obesity in rats, possibly through modulation of FAS and PTP-1B signaling due to the presence of flavonoids and lupeol.

Potential involvement of PPAR α activation in diminishing the hepatoprotective effect of fenofibrate in NAFLD

“…Balasubramanian et al [2] demonstrated that GF (18 mg/kg/day) orally for 30 days significantly reduce HFD induced gain in BW of rats. Also, Gupta et al [29] showed that GF deceased BW and LI in male Wistar rats fed HFD for 10 weeks which received GF (50 mg/kg/day) in the last 3 weeks of the experiment. This effect may be due to the prevention of pathological mechanisms responsible for excessive fat accumulation, dyslipidemia and weight gain, possibly by increasing leptin sensitivity, providing anorexic effect, and increasing energy expenditure.…”

In-vitro osteoblast proliferation and in-vivo anti-osteoporotic activity of Bombax ceiba with quantification of Lupeol, gallic acid and β-sitosterol by HPTLC and HPLC

“…Stem bark of the plant is reported to contain lupeol, β-sitosterol, shamimicin [ 21 ], ceibanephthaquinone, silamiln-A and silamalin-B [ 22 ], magniferin, epicatechin-3-O-b-xylopyra-noside, epicatechin-7-O-b-xylopyranosid [ 23 ], shamiminol, stigmasta-3,5-diene, lupenone and opuntiol [ 24 ]. The plant is reported as anti-inflammatory, anti-oxidant, anti-obesity, hepatoprotective, hypoglycemic and hypotensive activities [ 25 , 26 ]. B. ceiba is used for inflammatory diseases such as asthma, diabetes, wounded glandular swelling [ 27 ].…”

Bombax ceiba flowers extract ameliorates hepatosteatosis induced by ethanol and relatively moderate fat diet in rats

“…The Phytochemical compositions of methanol extract of the Egyptian B. ceiba flowers indicate the presence of alkaloids, flavonoids, glycosides, saponine, polyphenols, tannins, proteins, carbohydrates, lipids, amino acids and coumarins [33,34]. These valuable compounds offer the potent antioxidant and hypolipedimic properties of BCE in the liver tissues [35]. In our study, we examined the efficacy of these constituents in curing the alcohol addiction that were accompanied by fat intake in comparison to the effect of the standard drug; Fluvastatin.…”

“…This explains that the extract may be preventing the pathological mechanisms susceptive for excessive fat accumulation and weight gain due to the presence of bioactive components like alkaloids and glycosides. In addition, the extract is possibly increasing leptin sensitivity, providing anorexic effect and increasing energy expenditure [35,37]. The liver is the primary organ responsible for ethanol metabolism and it is susceptible to alcohol's toxic effects.…”