Post-thaw storage of sperm from various salmonid species

Abstract

Summary The effects of post‐thaw storage on the motility and fertilizing capacity of cryopreserved sperm were investigated in four salmonid species, including the Adriatic grayling (Thymallus thymallus; five males), rainbow trout (Oncorhynchus mykiss; six males), marble trout (Salmo marmoratus; five males) and the brown trout (Salmo trutta m. Fario; six males). The sperm of rainbow trout and marble trout was cryopreserved in the presence of either methanol or dimethyl‐sulfoxide (DMSO) as a cryoprotectant; for the sperm of the Adriatic grayling and brown trout, only methanol was used. A 1‐h storage of sperm did not reduce the fertilizing capacity of the three species, whereas in the marble trout a gradual decrease in the percentage of eyed eggs in parallel with post‐thaw storage time was observed. The use of methanol was found to result in higher fertilization than that of DMSO in the rainbow trout and marble trout. Remarkably high post‐thaw motility expressed in % motile cells was observed following post‐thaw storage of brown trout sperm for 10 (53 ± 12%) or 60 (56 ± 15%) min. The results indicate that immediate use of cryopreserved sperm for fertilization following thawing is not mandatory to attain successful fertilization.

Retracted: Effect of Post‐Thaw Storage Time on Motility and Fertility of Cryopreserved Beluga Sturgeon (Huso huso) Sperm

“…Our recent study on the Persian sturgeon, Acipenser persicus (Aramli and Nazari ) and also studies performed on the Adriatic grayling, rainbow trout, marble trout and brown trout (Horvath et al. ) demonstrated that the motility and fertilizing ability of cryopreserved sperm of these salmonid species can be preserved up to 60 min of post ‐ thaw storage of semen. In another study, Dreanno et al.…”

“…However, these parameters significantly decreased after 60 min of post-thaw storage. Our recent study on the Persian sturgeon, Acipenser persicus (Aramli and Nazari 2014) and also studies performed on the Adriatic grayling, rainbow trout, marble trout and brown trout (Horvath et al 2013) demonstrated that the motility and fertilizing ability of cryopreserved sperm of these salmonid species can be preserved up to 60 min of postthaw storage of semen. In another study, Dreanno et al (1997) reported that after cryopreservation of turbot sperm, the percentage of motile frozen-thawed spermatozoa was significantly lower than that of fresh sperm, whereas the velocity and duration of motion were not significantly modified.…”

Effect of postthaw storage time and sperm-to-egg ratio on fertility of cryopreserved brook trout sperm

“…To our knowledge, this is the first report concerning the examination of the effect of the storage period on the postthaw sperm motility parameters of brook trout. Preliminary studies performed on the Adriatic grayling, rainbow trout, marble trout, and brown trout [12] demonstrated that the fertilizing ability of cryopreserved sperm of these salmonid species can be preserved up to 60 minutes of postthaw storage of semen. Prolonged handling time of brook trout thawed semen could lead to better organization of hatchery work because the thawing procedure of several sperm samples for fertilization trials is time consuming.…”

Chilled and post‐thaw storage of sperm in different goldfish types

“…The importance of storage following thawing was also presented in various fish species (salmonids, sturgeon, perch etc.) (Aramli & Nazari, 2014;Bernáth et al, 2015;Horváth et al, 2015). The methods described above can support selective breeding and conservation programmes, as well as the hatchery practice in various fish (Aramli, Nazari, & Gharibi, 2015;Cabrita et al, 2010;Glenn et al, 2011).…”