Postcolumn derivatization in microcolumn liquid chromatography

Abstract

A postcolumn derivatization system was assembled using a commercially available Nano Y Connector with low dead volume for microcolumn liquid chromatography. Smooth baselines were achieved when postcolumn reagent solution and mobile phase were supplied by one microfeeder equipped with two syringes. Amino acids and inorganic anions were visualized by the postcolumn reaction with tolerable extracolumn band broadening.

Miniaturized Systems for Analytical Separations I: Systems Based on a Hydrodynamic Flow

“…This definition is accepted in this chapter, because the separation is carried out into capillaries in both cases. As T. Takeuchi recognized [22], one possible reason for the limited development of CLC (which began with Ishii's group, Japan, in 1974 [23]) lies in the use of electrically-driven pumping (Figure 4.4) for separation methods such as CZE and the other electrochromatographic modes (described in Chapter 5). The high resolution intended in CLC with the downsizing of the separation element containing the stationary phase (which follows the sequence shown in Figure 4.4) has the limitation of the pressure drop across the initial packed columns.…”

Development of Micro-Flow-Controlled Techniques and Novel Stationary Phases in Capillary Liquid Chromatography

“…As shown in Table 2, generally, LC can be classified into preparative LC, conventional LC, semi-micro-LC, micro-LC and nano-LC [17][18]. Until 2004, capillary columns in LC had been called microcolumns, microbore columns, small-bore columns and etc., where there was no specification made on their definition.…”

“…Until 2004, capillary columns in LC had been called microcolumns, microbore columns, small-bore columns and etc., where there was no specification made on their definition. In 2004, Takeuchi defined capillary LC as those involving nano-LC and micro-LC [14,18]. Saito et al, on the other hand, published a tremendously comprehensive review specifically on capillary columns in liquid chromatography (excluding electrodriven LC, i.e.…”

Novel devices for solvent delivery and temperature programming designed for capillary liquid chromatography

“…However, this miniaturized technique requires instrumental scaling for the maximum performance of the chromatographic system. The pumps needs to produce a low flow rate (μL/min) precisely and accurately, the connecting tube must have low extra‐column volume, and the sample introduction devices and detectors must be compatible with the capillary columns to avoid dispersion of the chromatographic band .…”