Postcolumn determination of polyphenolic antioxidants in Cirsium vulgare (Savi) Ten. extracts

Abstract

Novel methods for the determination of polyphenolic antioxidants present in extracts from inflorescences of Cirsium vulgare (Savi) Ten. based on ultra-high performance liquid chromatography with photodiode array and chemiluminescence detection have been developed. Under the optimized conditions of chromatographic separation the analytical characteristic of the method was performed. The proposed method was successfully applied to the determination of ten polyphenols present in inflorescences of Cirsium vulgare. A comparison of the contents of analytes in extracts prepared by using various extraction media (methanol, ethanol, 70% methanol, 70% ethanol, and water) was carried out for the first time. For the postcolumn detection of scavenging activity of polyphenolic antioxidants against reactive oxygen species (H O , OH, O ) three systems based on chemiluminescence of luminol were used. A review of the current scientific literature shows that this is the first report on the application of luminol-based postcolumn detection for the on-line investigation of OH scavenging activity. The main compound determined in extracts from inflorescences of Cirsium vulgare was apigenin 7-O-glucuronide, whereas the highest antioxidant activity was observed for chlorogenic acid, luteolin 7-O-glucoside, and apigenin.

Chemical fingerprint and bioactivity evaluation of Globularia orientalis L. and Globularia trichosantha Fisch. & C. A. Mey. using non‐targeted HPLC‐ESI‐QTOF‐MS approach

“…Based on its MS information and its main fragments yielded at m/z 268 and 269 corresponding to apigenin, it was tentatively identified as apigenin-O-glucuronide methyl ester. Despite the fact that it has not been previously reported in the Globulariaceae family, this compound has been characterised in other plant samples 48. The same occurred with peak 97 which was identified in the two samples for the first time as an apigenin derivative, and it was tentatively characterised as tri-O-methylvitexin.…”

Natural Compounds as a Strategy to Optimize “In Vitro” Expansion of Stem Cells

“…Cirsium vulgare is a common plant belonging to Asteraceae family whose main components are flavonoids and phenolic acids 137 . The effects of a C. vulgare hydroethanolic extract (200-1000 μg/mL) and its main component, di- (2-ethylhexyl) phthalate (100-600 μM) have been investigated on the proliferation and pluripotency of neonatal rat hippocampus-derived NSCs.…”

Chlorogenic Acid of Cirsium japonicum Resists Oxidative Stress Caused by Aging and Prolongs Healthspan via SKN-1/Nrf2 and DAF-16/FOXO in Caenorhabditis elegans

“…Chlorogenic acid (CA) is widely distributed in plants, fruits, and vegetables [ 26 ], and has been reported to exhibit various health benefits, having antioxidant, anti-inflammatory, antimicrobial, anticardiovascular, and anticancer properties [ 17 , 27 , 28 , 29 , 30 ]. In particular, CA is involved in a comprehensive antioxidation mechanism, directly scavenging free radicals by its polyhydroxy structure, and activating the oxidative signaling pathway by regulating gene expression and endogenous oxidase systems [ 30 ].…”