Posterior Hyaloid Detachment and Internal Limiting Membrane Peeling Assisted by Anthocyanins From Acai Fruit (Euterpe Oleracea) and 10 Other Natural Vital Dyes

Chen, Jane; Ferreira, Magno Antônio; Farah, Michel Eid; Carvalho, André Maia de; Ferreira, Raquel Eustaquio Alves; Filho, Milton Nunes de Moraes; Lima-Filho, Acácio Alves Souza; Lago, João Henrique G.; Sartorelli, Patrı́cia; Rodrigues, Eduardo Büchele; Ferreira, Eber Lopes; Peris, Cristiane S.; Maia, Maurício

doi:10.1097/iae.0b013e3182618a6d

Cited by 11 publications

(7 citation statements)

References 25 publications

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“…30 A recent study showed that the anthocyanin dye of the açaí fruit was efficient for posterior hyaloid and internal limiting membrane staining in human cadaveric eyes. 17 The results of this study confirm that açaí extract is effective in increasing stromal stiffness and resistance to thermal heating in rabbit corneas. Mechanical characterization of corneas by the three-point flexural test shows an increase in the elastic modulus by 10.6 times in the corneas treated with açaí extract for 2 h compared with the control corneas.…”

Section: Discussionsupporting

confidence: 86%

See 1 more Smart Citation

Characterization of Rabbit Corneas Subjected to Stromal Stiffening by the Açaí Extract (Euterpe oleracea)

Bersanetti

Bueno

Morandim-Giannetti

et al. 2016

Current Eye Research

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Section: Discussionsupporting

confidence: 86%

“…Chen et al 17 successfully used the flavonoid/ anthocyanin dye of the açaí fruit in an ophthalmologic application to stain and facilitate the posterior hyaloid detachment and internal limiting membrane peeling in human eyes.…”

Section: Introductionmentioning

confidence: 99%

Characterization of Rabbit Corneas Subjected to Stromal Stiffening by the Açaí Extract (Euterpe oleracea)

Bersanetti

Bueno

Morandim-Giannetti

et al. 2016

Current Eye Research

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“…Acai fruit is the most promising of a group of naturally occurring dyes that have recently been investigated. 117,118 It is a purple-colored anthocyanin dye that preferentially stains the ILM and recently underwent human trial (Clinicaltrials.gov: NCT02691429) with the results yet to be published.…”

Section: Investigational Dyesmentioning

confidence: 99%

Vital Dyes in Vitreomacular Surgery

Bracha¹,

Ciulla²,

Baumal³

2018

Ophthalmic Surg Lasers Imaging Retina

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Vital dyes contain complex molecules with chromophores that stain living tissues and have greatly enhanced identification and removal of transparent vitreoretinal tissues during surgery. Several "chromovitrectomy" dyes are frequently used by vitreoretinal specialists, including indocyanine green, trypan blue, brilliant blue G and triamcinolone acetonide; other dyes are also under investigation. Currently available chromovitrectomy dyes have their limitations, and of particular concern is their possibility for acute and chronic toxicity to the neurosensory retina and retinal pigmented epithelium. The potentially irreversible acute toxicity and other limitations, such as lack of long-term safety profiles, highlight the need for a review of the current literature and for further advancements.

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“…The antioxidant effects of anthocyanins theoretically could quench the singlet oxygen, which is released during chromovitrectomy [ 5 – 7 ]. The antioxidant property of the acai dye, associated with its high affinity for the internal limiting membrane (first observed in cadaveric eyes by our research team), became the basis for the development of a novel dye [ 8 ].…”

Section: Introductionmentioning

confidence: 99%

Analysis of Anthocyanins Extracted from the Acai Fruit (Euterpe oleracea): A Potential Novel Vital Dye for Chromovitrectomy

Peris

Caiado

Lima-Filho

et al. 2018

Journal of Ophthalmology

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Purpose To classify and quantify anthocyanins in a vital dye extracted from the acai fruit (Euterpe oleracea), adjust pH and osmolarity, and perform lyophilization to develop a new chromovitrectomy dye. Methods Three dye concentrations 10%, 25%, and 35% (equivalent to 100, 250, and 350 mg of lyophilized acai fruit pulp extract samples) were evaluated when diluted in 1 ml of phosphate-buffered solution (pH 7 and 300 mOsm). The dye was analyzed by mass spectrometry and high-performance liquid chromatography (HPLC) to identify and quantify anthocyanins molecules. Results The pH and osmolarity correction and lyophilization were performed without damaging the anthocyanin molecular structure. Mass spectrometry confirmed the presence of five anthocyanins in the three concentrations of the dye. Cyanidin-3-O-glucoside was the major anthocyanin found. HPLC showed that the concentration of anthocyanin was similar, independent of the dye concentration tested. Conclusions Lyophilization and the correction of pH and osmolarity (7.00 and 300 mOsm, resp.) were performed successfully. Five anthocyanins are present in the dye from the acai fruit. The major anthocyanin is cyanidin-3-O-glucoside. Independent of the dye concentration tested, the anthocyanin concentration was similar. Standardized chemical characteristics of this new dye may allow its use during chromovitrectomy in humans.

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Posterior Hyaloid Detachment and Internal Limiting Membrane Peeling Assisted by Anthocyanins From Acai Fruit (Euterpe Oleracea) and 10 Other Natural Vital Dyes

Abstract: Anthocyanin dye of the acai fruit (E. oleracea) and the dyes from cochineal (D. coccus) and chlorophyll extract from alfalfa (M. sativa) resulted in the best capability for posterior hyaloid and ILM staining in human cadaveric eyes and may be a useful tool for vitreoretinal surgery.

Cited by 11 publications

References 25 publications

Characterization of Rabbit Corneas Subjected to Stromal Stiffening by the Açaí Extract (Euterpe oleracea)

Characterization of Rabbit Corneas Subjected to Stromal Stiffening by the Açaí Extract (Euterpe oleracea)

Vital Dyes in Vitreomacular Surgery

Analysis of Anthocyanins Extracted from the Acai Fruit (Euterpe oleracea): A Potential Novel Vital Dye for Chromovitrectomy

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