Potassium and tetraphenylphosphonium ion-selective electrodes for monitoring changes in the permeability of bacterial outer and cytoplasmic membranes

Yasuda, Keiko; Ohmizo, Chie; Katsu, Takashi

doi:10.1016/s0167-7012(02)00255-5

Cited by 31 publications

(30 citation statements)

References 12 publications

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“…To disrupt the outer membrane of E. coli, EDTA at 0.1mM (final concentration) was added. Cetyltrimethylammonium bromide (CTAB) at 0.25 mg/ml was used as a positive control, to determine the 100% level of K + efflux from bacteria (Yasuda et al, 2003).…”

Section: E Colimentioning

confidence: 99%

Susceptibility of Escherichia coli, Salmonella sp. and Clostridium perfringensto organic acids and monolaurin

Skřivanová¹,

Marounek²,

Benda³

et al. 2006

Vet. Med.

176

128

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The antimicrobial activity of fatty acids, monolaurin, citric, succinic, fumaric, malic and lactic acid was determined in cultures of two strains of Escherichia coli, three strains of Salmonella sp. and two strains of Clostridium perfringens. Antimicrobial activity was expressed as minimum inhibitory concentration (MIC) that prevented growth and glucose utilization in treated cultures. Caprylic acid was the only acid inhibiting glucose utilization in all cultures. Its MIC varied from 1 to 3 mg/ml. Strains CCM 3954 and CCM 4225 of E. coli were inhibited also by capric acid at 5 mg/ml. Strains CCM 4435T and CNCTC 5459 of Cl. perfringens were inhibited by medium-chain fatty acids (C8 to C14), oleic acid and one strain also by linoleic acid. The minimum MICs were those of lauric and myristic acid (between 0.1 and 0.2 mg/ml). Growth of Cl. perfringens, but not other bacteria, was inhibited also by monoglyceride of lauric acid (MIC = 3 mg/ml), and by citric acid (MIC = 4 mg/ml). Inhibitory effects of other acids were not observed at 5 mg/ml. Caprylic and lauric acid did not influence the K+ permeability of the cytoplasmic membrane in cells of E. coli CCM 4225 and Cl. perfringens CCM 4435T, respectively. In cultures of both strains of E. coli treated with caprylic acid at 5 mg/ml, and in those of Cl. perfringens CCM 4435T treated with lauric acid at 1 mg/ml, or with its monoglyceride at 5 mg/ml, the transmission electron microscopy revealed damage of cytoplasmatic structures. In cells of Cl. perfringens the separation of inner and outer membranes was apparent, the integrity of the outer membrane, however, was maintained. It can be concluded that medium-chain fatty acids are more efficient antimicrobials than other, more polar organic acids tested.

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Section: E Colimentioning

confidence: 99%

Susceptibility of Escherichia coli, Salmonella sp. and Clostridium perfringensto organic acids and monolaurin

Skřivanová¹,

Marounek²,

Benda³

et al. 2006

Vet. Med.

176

128

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“…The effects are higher on Gramnegative bacterium where an action on the membrane acid leads to an increase in fluidity and permeability, causing the release of lipopolysaccharide. [13,14] …”

Section: Discussionmentioning

confidence: 99%

The antibacterial activity of contact lens solutions against microbial keratitis

Budiman¹,

Fauzi²,

Sulistiyaningsih³

et al. 2017

Natl J Physiol Pharm Pharmacol

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Background: Contact lens has a growing popularity among people with vision impairment as well as for cosmetic reasons. However, its contamination can cause microbial keratitis. This study aimed to test the effectiveness of three antimicrobial contact lens solutions against microbial keratitis and to determine the contact time and adjustments in the existing label of multipurpose solutions. Aims and Objectives: To study the antimicrobial activity of three locally available contact lens solutions against Pseudomonas aeruginosa and Staphylococcus aureus and determined their contact time. Materials and Methods: Three bottles of each contact lens solution were evaluated using a single-blind controlled experiment method. These test solutions were categorized based on their identified disinfecting ingredient. This study evaluated the antibacterial activity of three contact lens solutions against P. aeruginosa and S. aureus at different contact times of 1, 3, 6, and 12 h. Results: All samples of contact lens solutions tested showed excellent antimicrobial activity. The contact lens solution B had the highest antibacterial activity against S. aureus and P. aeruginosa with a contact time of 6 h. Conclusion: The results showed that all the contact lens solutions demonstrate antimicrobial activity even after 24 h of exposure. Contact lens solutions containing polyhexamethylene biguanid as the active agent are found to have excellent antimicrobial activity and a desirable contact time.

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“…The initial interactions of PHMB and the membrane is concentrated around such proteins, leading to a loss of their function by inflicting changes in their boundary phospholipid environment. This interaction leads to increased fluidity, permeability, and loss of integrity of membranes, followed by the disruption of the bacterial metabolism and the death of the organism [35] [36]. The ability of PHMB to create single lipid domains within heterogeneous lipid-bilayers is clearly a function of polymer chain length with longer polymers, being able to form the larger domains and hence the greater perturbation of membrane function.…”

Section: Discussionmentioning

confidence: 99%

Polyhexamethylene Biguanide in Vaginal Solution Is Effective in the Treatment of Vulvovaginal Candidiasis: A Pilot Study

Biamonti¹,

Saracino²

2017

OJOG

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Vaginitis is one of the most recurrent conditions that afflict women and require medical care. Vulvovaginal candidiasis (VVC) is frequently related to vaginal carriage of Candida albicans, an opportunistic polymorphic fungus, which colonizes the human skin, the mucosa and the reproductive tract. Actually, standard treatments to counteract vaginitis include several topical and oral treatments. The aim of this study was to evaluate the efficacy of a new biguanide compound, polyhexamethylene biguanide (PHMB) in avaginal solution, in women affected by vaginal candidiasis. For this reason, 40 women showing the symptoms previously ascribed to vaginal candidiasis were recruited and treated with a single PHMB dose of vaginal solution. After a single dose of treatment, 80% of them had a complete resolution of the fungal infection; furthermore, all of them reported clinical benefits, statistically significant reduction in both clinical signs and symptoms of candidiasis and a score's reduction of 50%.

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Potassium and tetraphenylphosphonium ion-selective electrodes for monitoring changes in the permeability of bacterial outer and cytoplasmic membranes

Cited by 31 publications

References 12 publications

Susceptibility of Escherichia coli, Salmonella sp. and Clostridium perfringensto organic acids and monolaurin

Susceptibility of Escherichia coli, Salmonella sp. and Clostridium perfringensto organic acids and monolaurin

The antibacterial activity of contact lens solutions against microbial keratitis

Polyhexamethylene Biguanide in Vaginal Solution Is Effective in the Treatment of Vulvovaginal Candidiasis: A Pilot Study

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