Potensi Antibakteri Ekstrak Diethyl Ether Daun Mahkota Dewa (Phaleria macrocarpa (Scheff.) Boerl) terhadap Bakteri Pseudomonas aeruginosa dan Staphylococcus aureus

Staphylococcus aureus and Pseudomonas aeruginosa are opportunistic pathogenic bacteria that are the main causes of nosocomial infections. These bacteria can infect almost every tissue of the body and there were 15% cases of infection in hospitals. Urinary tract infections, even sepsis, where the death rate reaches 50%. Inappropriate use of antibiotics raises resistance. About 10% of bacterial isolates are generally expressed as S. aureus and P. aeruginosa Multidrug Resistant (MDR). Sargassum brown seaweed has many potential antimicrobial compounds. This research aims to screen the antibacterial active compounds of Sargassum crassifolium seaweed against S. aureus and P. aeruginosa MDR bacteria. S. crassifolium collected from waters of Karimunjawa Islands, Jepara. The research was conducted by laboratory experimental methods. Sample was extracted with diethyl ether, methanol, ethanol and chloroform. The MIC value is done by measuring the diameter of the inhibitory zone in the antibacterial activity test of the agar diffusion method. Furthermore, extracts at MIC concentrations were tested for the antibacterial activity of the diluted method by measuring bacterial OD by spectrophotometric methods. The extract with the best antibacterial activity was performed spectral analysis by GC-MS method. The results showed that the different extracts had different MIC values (p <0.05). Extracts with high antibacterial activity are extracts from diethyl ether solvent. The extract has a MICP value of P. aeruginosa 12.7 mg/ml and S. aureus 8.4 mg/ml. P. aeruginosa has exponential growth at 12 hours and death at 44 hours. While exponential S. aureus was at 16 hours and death at 36 hours. Spectral analysis of S. crassifolium extract of diethyl ether solvent showed the composition of the presence of eicosane compounds (16.22% ), dotriacontane (11.27%), nanocosane (11.09%), dicosane (9.85%), 10.13-octadeadienoic acid (9.52%). 2-butyloctanol (6.33%), pentatriacontane (5.4%), tritriacontane (5.07%), tricosane (1.6%)

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In vitro antibacterial study and spectral analysis of brown seaweed Sargassum crassifolium extract from Karimunjawa Islands, Jepara

Setyati

Pramesti

Susanto

et al. 2020

IOP Conf. Ser.: Earth Environ. Sci.

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Identification <i>Escherichia coli</i> on some fisheries commodities in the traditional market of Mamuju, West Sulawesi

Jamaluddin

Nurfadilah

Sunarti

2021

Akuatikisle: J. Aqua. Coast. & Isle

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Fish is a source of high-quality protein. Protein in fish has a complete composition and amount of essential amino acids. However, fish is a type of food that is susceptible to biological damage and is susceptible to microbial contamination so that it quickly decays. One of the most important microorganisms in spoilage of fish meat is Escherichia coli bacteria. This study aims to determine the presence of Escherichia coli bacteria in scad, tuna and skipjack tuna. The research was carried out from July to September 2021. The research method used was a sample survey method, sampling was carried out at 3 market locations, namely the TPI market and the tasiu market. Furthermore, the samples were analyzed at the Laboratory of Fish Quarantine and Quality Control Center (BKIPM) Mamuju, West Sulawesi. The results showed that the largest E.coli content was found in tuna 1 in the TPI market, which was 20 MPN/g, and the smallest E.coli content was < 3 MPN/g, namely scad fish 1 in the tasiu and TPI markets, tuna 1 and 2 in the TPI market, tuna 2 in TPI market.

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Potensi Antibakteri Ekstrak Diethyl Ether Daun Mahkota Dewa (Phaleria macrocarpa (Scheff.) Boerl) terhadap Bakteri Pseudomonas aeruginosa dan Staphylococcus aureus

Abstract: This study aims to test the antibacterial potential of diethyl ether extract of mahkota dewa leaves (Phaleria macrocarpa (Scheff.)

Cited by 2 publications

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In vitro antibacterial study and spectral analysis of brown seaweed Sargassum crassifolium extract from Karimunjawa Islands, Jepara

In vitro antibacterial study and spectral analysis of brown seaweed Sargassum crassifolium extract from Karimunjawa Islands, Jepara

Identification <i>Escherichia coli</i> on some fisheries commodities in the traditional market of Mamuju, West Sulawesi

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