Potensi Bakteri Simbion Endofit Mangrove Avicennia marina sebagai Antifouling

Abstract

Biofouling is micro-organism and makrofouling attached to the submerged substrate. Prevention of biofouling organism called antifouling.Tributyltin organotin was using to mixture antifouling paint. Antifouling paints containing toxic and environmentally unfriendly. Symbiotic bacteria have secondary metabolites, there are tanin, steroid, and triterpenoid. This research aimed to obtain antifouling bacteria through isolation process of bacteria obtained from the roots of mangrove Avicennia marina, and analyze the bacterial symbiont inhibitory zone of mangrove Avicennia marina as antifouling. The method was beginning with the isolation of bacteria roots of mangrove Avicennia marina, identification of symbiotic bacteria, and the antifouling activity test. Results obtained from the isolation of bacteria, there are 21 symbiotic endophytic on a sample of the inner root, and 15 epiphytic bacteria on a sample of the outer root. Endophytic bacteria inhibition zone test potential as antifouling from mangrove Avicennia marina that was from the root part that inhibitory zone size strong category for> 10 mm. Biofouling merupakan organisme mikro dan makrofouling yang menempel pada substrat terendam. Pencegahan terhadap organisme biofouling disebut antifouling. Antifouling menggunakan campuran cat Tributyltin organotin. Cat antifouling mengandung bahan beracun dan tidak ramah lingkungan. Bakteri simbion memiliki kandungan metabolit sekunder, seperti tanin, steroid dan triterpenoid. Penelitian ini bertujuan untuk memperoleh bakteri antifouling melalui proses isolasi bakteri yang diperoleh dari akar mangrove Avicennia marina dan menganalisis zona hambat bakteri simbion mangrove Avicennia marina sebagai antifouling. Metode yang digunakan diawali dengan isolasi bakteri akar mangrove Avicennia marina, identifikasi bakteri, dan uji aktivitas antifouling. Hasil yang diperoleh dari isolasi bakteri, yaitu 21 bakteri simbion endofit dan sampel akar bagian luar diperoleh 15 bakteri epifit. Uji zona hambat bakteri endofit yang berpotensi sebagai antifouling dari tumbuhan mangrove Avicennia marina yaitu memiliki ukuran zona hambat kategori kuat karena >10 mm.

Biodegradation Test of Rhodamine B by Mangrove Root Symbiont Bacteria Avicennia marina in Sea Panipahan, Muara Rokan, Panipahan Village, Riau

“…The purposive sampling method was used to conduct sampling, which is a technique based on the existence of certain objectives rather than strata, randomness, or regions (Sa'adah & Novitasari, 2022). By the sampling conditions, mangrove root samples were taken from 2 different Avicennia marina mangrove trees.…”

“…Pneumatophore mangrove root samples were dried and then crushed using a sterile mortar until smooth (Sa'adah & Novitasari, 2022). The results of the grinding were weighed 1g and put into an Erlenmeyer containing 9 ml sterile seawater, then 1 ml of suspension was put into 9 ml sterile seawater to make a dilution of 10-1, dilutions were made up to 10-5 (Modification of Yanti et al, 2021).…”

“…The clear zone around the bacterial isolate indicates that the bacterial isolate has the potential to degrade Rhodamine B. The clear zone is measured using a caliper with an accuracy of 0.05 mm (Sa'adah & Novitasari, 2022).…”

Karakteristik Morfologis dan Fisiologis Bakteri Endofit dari Akar Napas Tumbuhan Avicennia marina (fork) vierh di Mempawah Mangrove Park

“…Pengambilan sampel akar napas A. marina menggunakan metode sistematis (Systematic sampling) yaitu menelusuri kawasan Mempawah Mangrove Park dan menentukan 5 titik area A. marina (Adiarti, 2013). Setiap titik dipilih 5 pohon yang memiliki umur sekitar 1-2 tahun, pada setiap pohon diambil 3 akar napas.…”