Potent effects of, and mechanisms for, modification of crosstalk between macrophages and adipocytes by lactobacilli

Miyazawa, Kenji; He, Fang; Yoda, Kazutoyo; Hiramatsu, Masaru

doi:10.1111/j.1348-0421.2012.00512.x

Cited by 15 publications

(30 citation statements)

References 35 publications

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“…In the latter situation, strains such as CRL117, CRL350, CRL143 CRL575, CRL1446, and CRL66 could be useful. Miyazawa et al demonstrated that increased pro-inflammatory cytokines (IL-6, IL-12, and TNF-α) inhibited the differentiation of a fibroblast cell line (3T3L1) in adipocytes, suggesting that acute inflammation stimulated by lactobacilli could be the mechanism by which adipogenic differentiation is suppressed, leading to adipocyte hypertrophy (33). …”

Section: Discussionmentioning

confidence: 99%

“…In addition, it has been suggested that microbial products associated with immunobiotics (immunoregulatory probiotics) can also cross the intestinal barrier, being able to modulate distant tissues (such as adipose tissue) through the induction of changes in cytokine profiles (32). Miyazawa et al in an in vitro study using conditioned medium showed that different lactobacilli may suppress differentiation of preadipocytes through macrophage activation and alter the immune responses of macrophages to adipose cells (33). …”

Section: Introductionmentioning

confidence: 99%

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Specific Strains of Lactic Acid Bacteria Differentially Modulate the Profile of Adipokines In Vitro

et al. 2017

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Obesity induces local/systemic inflammation accompanied by increases in macrophage infiltration into adipose tissue and production of inflammatory cytokines, chemokines, and hormones. Previous studies have shown that probiotics could improve the intestinal dysbiosis induced by metabolic diseases such as obesity, diabetes, and metabolic syndrome. Microorganisms could (directly or indirectly) affect adipokine levels due to their capacity to induce translocation of several intestinal microbial antigens into systemic circulation, which could lead to metabolic endotoxemia or produce immunomodulation in different organs. The aim of the present study was to select non-inflammatory lactic acid bacteria (LAB) strains with the capacity to modulate adipokine secretion by the adipose tissue. We wish to elucidate the role of potential probiotic strains in the regulation of the cross talking between immune cells such as macrophages and adipose cells. Mouse macrophage cell line RAW 264.7 was used for evaluating the ability of 14 LAB strains to induce cytokine production. The LAB strains were chosen based on their previously studied beneficial properties in health. Then, in murine adipocyte culture and macrophage–adipocyte coculture, we determined the ability of these strains to induce cytokines and leptin secretion. Tumor necrosis factor alpha, interleukin 6 (IL-6), IL-10, monocyte chemoattractant protein-1, and leptin levels were measured in cell supernatants. We also performed the detection and quantification of leptin receptor (Ob-Rb) expression in macrophage cell lines stimulated by these LAB strains. Differential secretion profile of cytokines in macrophage cells induced by LAB strains was observed. Also, the levels of Ob-Rb expression diverged among different LAB strains. In LAB-stimulated coculture cells (adipocytes and macrophages), we observed differential production of leptin and cytokines. Furthermore, we detected lower production levels in single culture than cocultured cells. The principal component analysis showed an association between the four clusters of strains established according to their inflammatory profiles and leptin adipocyte production and leptin receptor expression in macrophages. We conclude that coculture is the most appropriate system for selecting strains with the ability to modulate adipokine secretion. The use of microorganisms with low and medium inflammatory properties and ability to modulate leptin levels could be a strategy for the treatment of some metabolic diseases associated with dysregulation of immune response.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Specific Strains of Lactic Acid Bacteria Differentially Modulate the Profile of Adipokines In Vitro

et al. 2017

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“…rhmanosus LA-2 mediated up-regulation was unknown. In addition, we previously used the conditioned medium of murine macrophage-like cell line J774.1 cultured with LGG or TMC0356 strains to stimulate mouse preadipocyte cell line 3T3-L1 and found a suppressed lipid accumulation and reduced PPAR-γ mRNA expression [ 19 ]. Moreover, the J774 cells treated with Lactobacillus GG or L .…”

Section: Discussionmentioning

confidence: 99%

“…gasseri TMC0356 improved the production of IL-6 and IL-12 [ 19 ]. The conditioned medium from lactobacilli-cultured J774.1 cells transferred to the preadipocyte cell line 3T3-L1 significantly suppressed lipid accumulation and decreased peroxisome proliferator activated receptor γ (PPAR-γ) [ 19 ]. Similarly, Park et al [ 20 ] showed that exposure of 3T3-L1 adipocytes to L .…”

Section: Introductionmentioning

confidence: 99%

Advanced Application of Porcine Intramuscular Adipocytes for Evaluating Anti-Adipogenic and Anti-Inflammatory Activities of Immunobiotics

et al. 2015

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We previously established a clonal porcine intramuscular preadipocyte (PIP) line and we were able to establish a protocol to obtain functional mature adipocytes from PIP cells. We hypothesized that both PIP cells and mature adipocytes are likely to be useful in vitro tools for increasing our understanding of immunobiology of adipose tissue, and for the selection and study of immunoregulatory probiotics (immunobiotics) able to modulate adipocytes immune responses. In this study, we investigated the immunobiology of PIP cells and mature adipocytes in relation to their response to TNF-α stimulation. In addition, we evaluated the possibility that immunobiotic microorganisms modify adipogenesis and immune functions of porcine adipose tissue through Peyer’s patches (PPs) immune-competent cells. We treated the porcine PPs immune cells with different probiotic strains; and we evaluated the effect of conditioned media from probiotic-stimulated immune cells in PIP cells and mature adipocytes. The Lactobacillus GG and L. gasseri TMC0356 showed remarkable effects, and were able to significantly reduce the expression of pro-inflammatory factors and negative regulators (A20, Bcl-3, and MKP-1) in adipocytes challenged with TNF-α. The results of this study demonstrated that the evaluation of IL-6, and MCP-1 production, and A20 and Bcl-3 down-regulation in TNF-α-challenged adipocytes could function as biomarkers to screen and select potential immunobiotic strains. Taking into consideration that several in vivo and in vitro studies clearly demonstrated the beneficial effects of Lactobacillus GG and L. gasseri TMC0356 in adipose inflammation, the results presented in this work indicate that the PIP cells and porcine adipocytes could be used for the screening and the selection of new immunobiotic strains with the potential to functionally modulate adipose inflammation when orally administered.

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“…Because pulmonary TNF‐α gene expression was upregulated by LGG administration in the present study, the resultant proinflammatory status may have led to further increases in body weight and fat. On the other hand, our previous study showed that LGG administration reduces lipid accumulation in the murine pre‐adipocyte cell line 3T3‐L1 thorough TNF‐α production by the murine macrophage‐like cell line J774.1 . Unfortunately, we did not examine markers associated with obesity in adipose tissue in the present study.…”

Section: Bacterial Profiles Of Each Group According To T‐rflp Analysismentioning

confidence: 75%

Influence of orally administered Lactobacillus GG on respiratory immune response in a murine model of diet‐induced obesity

Miyazawa

Yoda

Kawase

et al. 2015

Microbiology and Immunology

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Mice with diet-induced obesity were fed with Lactobacillus rhamnosus GG (LGG) suspended in saline or saline alone (control mice). Pulmonary mRNA expression of IFN-g; IFN-a receptor 1; CD247 antigen; killer cell lectin-like receptor subfamily K, member 1; TNF-a; IL-12 receptor b1 and IL-2 receptor b, and the proportion of Lactobacillales in feces were significantly greater in the LGG group than in the control mice (P < 0.05 and P < 0.01, respectively). These results suggest that LGG alters the respiratory immunity of obese subjects through having a potent impact on intestinal immunity.

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Potent effects of, and mechanisms for, modification of crosstalk between macrophages and adipocytes by lactobacilli

Abstract: ABSTRACT

Cited by 15 publications

References 35 publications

Specific Strains of Lactic Acid Bacteria Differentially Modulate the Profile of Adipokines In Vitro

Specific Strains of Lactic Acid Bacteria Differentially Modulate the Profile of Adipokines In Vitro

Advanced Application of Porcine Intramuscular Adipocytes for Evaluating Anti-Adipogenic and Anti-Inflammatory Activities of Immunobiotics

Influence of orally administered Lactobacillus GG on respiratory immune response in a murine model of diet‐induced obesity

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