Potential Alternative Hosts and Transmissibility of Potato virus Y

Rizk, Mohamed N.; Ketta, H. A.; Shabana, Yasser M.

doi:10.21608/jppp.2020.133232

Cited by 4 publications

(4 citation statements)

References 6 publications

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“…The seedlings of papaya were grown and maintained in nursery begs under insect rearing gauge to avoid cross contamination and infestation of unwanted insects. Preparation of phosphate buffer About 1.781g of Sodium phosphate monobasic (Na2HPO2.2H20) were dissolved in 1 Liter of dH2O (deionized water) to make Na solution and 1.362g of Sodium hydrogen phosphate (KH2PO4.2H20) was dissolved in 1liter of dH2O (deionized water) to make Solution K. Then 49 ml of solution K and 51 ml of solution Na were mixed to prepare phosphate buffer as described by Rizk et al [20]. Preparation of sap for inoculation About 5 g of symptomatic papaya were taken and crushed using10 ml phosphate buffer in sterilized pestle and mortar.…”

Section: Pathogenicity Test For Confirmation Of Viral Infection At Te...mentioning

confidence: 99%

Intensity of papaya ring spot disease in district Hyderabad Pakistan and its management using antiviral compounds

Qazi¹

2023

PAB

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Papaya ring spot disease caused by Papaya ringspot virus (Genus: Potyvirus, Family: Potyviridae) is a major yield limiting factor of papaya in Pakistan. In severe attack of the disease crop can be lost completely. The aim of the study was to estimate prevalence of this destructive disease in district Hyderabad and to evaluate the effectiveness of Azaguanine and Ribavirin for managing the disease. During survey in Hyderabad, the incidence (%) and severity (score) of the disease was determined. Sap obtained from disease plants was mechanically inoculated on 30 days old test plants and induction of typical symptoms was noted. After induction of local lesions both chemicals at concentration of 100, 500, 1000 µM were applied with five replications. Non-significant difference among disease incidence (%) was recorded in Hyderabad (Df = 4, F = 0.49, P = 0.7435). Severity of the disease was significantly varying in the surveyed fields in Hyderabad (Df = 4, F= 1.59, P = 0.1843). Mechanically inoculated plants showed 100±0 % incidence and 2.2±0.5 severity score. Among various tested concentrations of Azaguanine and Ribavirin, only 1000 µM significantly decreased 40 ± 3.3 % incidence and 0.4 ± 0.33 severity of the disease. It is concluded that disease is prevailing in Hyderabad and application of Azaguanine and Ribavirin at 1000 µM could reduce the disease. Furthermore, it is recommended at antiviral compounds shall be included in disease management strategy and additional studies shall be undertaken to address the mechanism of the antiviral compounds to control the disease.

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Section: Pathogenicity Test For Confirmation Of Viral Infection At Te...mentioning

confidence: 99%

Intensity of papaya ring spot disease in district Hyderabad Pakistan and its management using antiviral compounds

Qazi¹

2023

PAB

View full text Add to dashboard Cite

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“…Preparation of phosphate buffer 1.781 g of Sodium phosphate monobasic (Na2HPO2.2H20) was dissolved in 1L of deionized water (dH2O) to make the Na solution and 1.362 g of Potassium hydrophosphate (KH2PO4.2H20) was dissolved in 1L of deionized water (dH20) to constitute the K solution. Phosphate buffer was then made by adding 51 ml of the Na solution to 49 ml of the K solution, as reported by Rizk et al (2020).…”

Section: Pathogenicity Test For Confirmation Of Viral Infection At Te...mentioning

confidence: 99%

Chemotherapy of Tomato Mosaic Disease using Ribavirin and 8-Azaguanine

et al. 2023

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Tomato mosaic disease (ToMD) caused by Tomato mosaic virus (ToMV) is a major threat to tomato production globally. In Pakistan, tomato is one of the most popular vegetables, and ToMD causes significant losses in fruit production, quality, and size. Various management strategies are implemented to control the disease. In this study, the efficacy of chemotherapy using ribavirin and 8-azaguanine was investigated. Seedlings of tomato plants were inoculated with sap from symptomatic tomato plants using the sap transmission technique to test the pathogenicity of the virus. Disease incidence and severity were recorded at weekly intervals for one month after inoculation. Ribavirin and 8-azaguanine were applied to tomato plants at different concentrations, and disease incidence and severity were observed weekly for one month after treatment. The results showed that the mechanically inoculated plants exhibited characteristic symptoms of ToMD, such as a mosaic pattern of color on the leaves. The disease incidence did not reduce with the application of ribavirin and 8-azaguanine, but the severity of the disease was significantly reduced. The plants treated with 1000 µM of ribavirin and 8-azaguanine had significantly lower severity scores compared to the untreated plants. However, the treated plants did not recover from the disease. The study found that ribavirin and 8-azaguanine are effective chemotropic agents in reducing the severity of ToMD, although they cannot cure the disease completely. Further studies are required to investigate the underlying mechanism of how ribavirin and 8-azaguanine suppress the symptoms of ToMD

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“…The PVY genome consists of a monopartite single-stranded RNA enclosed by coat protein subunits, forming a flexuous filamentous particle (Inoue-Nagata et al 2022). Transmission occurs naturally by a diverse array of aphid vector species in a non-persistent manner (Davie et al 2017; Rizk et al 2020). PVY exhibits a wide range of plant hosts, including agricultural relevant species such as tomato, potato, peppers, and tobacco, as well as numerous weed species (Edwardson and Christie 1997; Jeffries 1998).…”

Section: Introductionmentioning

confidence: 99%

Emergence of potato virus Y outbreaks in tomatoes in Brazil, the disease and spread

Lucena,

Nakasu,

Pereira

et al. 2024

Preprint

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The emergence of 'Mexican Fire' disease in Brazilian tomato fields, attributed to potato virus Y (PVY), has raised concerns. Characterized by severe necrosis on median leaves, the definitive etiological agent of this disease remained unverified despite PVY detection in symptomatic plants. Our study aimed to elucidate the causal agent, occurrence, spread, and symptomatology of Mexican Fire. Deep sequencing of tomato leaves with typical necrotic symptoms confirmed the association with PVY, reinforcing its role as the causal agent. Serological tests with a PVY-specific polyclonal antibody consistently correlated symptoms with virus presence in a fresh market tomato field, with higher PVY incidence near older tomato and maize plants. Necrotic leaf distribution analysis revealed a predominant occurrence in median leaves, progressing upwards. Deep sequencing of symptomatic field samples exclusively detected PVY, reaffirming its role in symptom induction. Importantly, PVY inoculation under field and greenhouse conditions fulfilled Koch's Postulates, triggering leaf necrosis. Our findings unequivocally establish PVY as the causal agent of Mexican Fire disease, shedding light on its etiology, incidence, spread, and symptom expression, crucial for effective disease management strategies.

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Potential Alternative Hosts and Transmissibility of Potato virus Y

Abstract: To study the potential alternative hosts to Potato virus Y (PVY), thirteen different plant species/cultivars, Solanum tuberosum cv. Spounta L., S. tuberosum cv.

Cited by 4 publications

References 6 publications

Intensity of papaya ring spot disease in district Hyderabad Pakistan and its management using antiviral compounds

Intensity of papaya ring spot disease in district Hyderabad Pakistan and its management using antiviral compounds

Chemotherapy of Tomato Mosaic Disease using Ribavirin and 8-Azaguanine

Emergence of potato virus Y outbreaks in tomatoes in Brazil, the disease and spread

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