Potential anti‐inflammatory and antioxidant effects of Citrus aurantium essential oil against carbon tetrachloride‐mediated hepatotoxicity: A biochemical, molecular and histopathological changes in adult rats

Abstract

The present study aimed (1) to investigate the chemical composition as well as the antiinflammatory properties and in vitro antioxidant activity of Citrus aurantium peel essential oil (pEOCa) and (2) to evaluate its potential effect in vivo. The main results showed that the major components of pEOCa are Limonene and Linalool. Additionally, DPPH scavenging ability and β-carotene bleaching inhibition tests confirmed the antioxidant capacity of pEOCa. Our oil reduced the production of NO by LPS-stimulated RAW264,7 macrophages in a concentrationdependent. This inhibition occurred at a transcriptional level. pEOCa in CCl 4 treated rats alleviated hepatotoxicity as monitored by the improvement of hepatic oxidative stress biomarkers levels plasma biochemical parameters, and DNA molecule aspect. Furthermore, the mRNA gene expression of Cu-Zn SOD, CAT, and GPx increased under CCl 4 + pEOCa exposure to reach the same value to the control. Similarly, antioxidant activities of these three enzymes changed in accordance with the mRNA levels. These results were confirmed by the histological results. It seems obvious that the treatment with pEOCa prevented liver damage induced by CCl 4 , thus preventing the harmful effects of free radicals. K E Y W O R D Santioxidant activity, beneficial effect,

Chemical composition and hepatoprotective effect of essential oil fromMyrtus communisL. flowers against CCL4-induced acute hepatotoxicity in rats

“…12 Hence, our results revealed that the injection of CCl 4 to rats caused a signicant increase in AST, ALT, ALP and LDH activities at day 14 of treatment. This is in agreement with previous reports, 43,46 suggesting that an extensive liver injury was occasioned by CCl 4 due to changes in their functional transition. These changes cause membrane permeability, and leads to the leakage of enzymes into extracellular space.…”

Novel non-specific lipid-Transfer Protein (TdLTP4) isolated from Durum wheat: Antimicrobial activities to control pathogen and spoilage bacteria and Anti-Inflammatory properties in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages

“…The cells were cultured in DMEM medium supplemented with 10% heat-inactivated FBS, 1% streptomycin/penicillin at 37 °C in a humidi ed atmosphere of 5% CO2. The cells were treated with TdLTP4 at different concentrations and then stimulated with 0.5 µg/mL LPS for 18 h (Ben Hsouna et al, 2018;.…”

“…Measurement of NO production RAW 264.7 cells (2 × 105 cells/well) were pre-incubated for 1 h with various concentrations of TdLTP4 and stimulated with LPS (0.5 µg/mL) at 37°C for 18 h in medium. NO levels were determined by measuring nitrite levels in the culture media using Griess reagent assay according to our previously described method (Tursun et al, 2016;, 2018.…”

“…Measurement of IL-6, IL-1β, TNF-α and IL-10 levels RAW264.7 cells (2 × 10 5 cells/well) were pretreated with different concentrations of TdLTP4 for 1 h and then stimulated with LPS (0.5 µg/mL) for 18 h. The concentration of IL-6, TNF-α, IL-10 and IL-1β were assayed using the ELISA kits according to the manufacturer's instructions (Ben Hsouna et al, 2018;.…”

Anticonvulsant Essential Oils and Their Relationship with Oxidative Stress in Epilepsy

“…The flowers of C. aurantium are used to treat various neurological disorders such as insomnia, epilepsy, and hysteria [48]. Some pharmacological activities have already been described for C. aurantium essential oil (CAEO) such as an anti-inflammatory agent [49], an anxiolytic [50], as a larvicide [51] and antioxidant [52].…”