Potential of Tissue Engineered Blood Vessel as Model to Study Effect of Flow and Wall Thickness on Cellular Communication

Ragaseema, V. M.; Columbus, Soumya; Ramesh, Renu; Krishnan, Lissy K.

doi:10.2174/2211542002666131209233849

Cited by 5 publications

(2 citation statements)

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“…Recently, the improvements of tissue engineering enable the fabrication of tissue-engineered blood vessels (TEBVs) for studying atherogenesis [41]. Ragaseema et al developed two-layered TEBVs by seeding endothelial cells and SMC on a biodegradable copolymer conduit in a perfusion system to obtain similar mechanical properties to native arteries [43], while Robert et al fabricated a TEBV by seeding vascular cells and adding LDL and TNFα under high or low shear stress, demonstrating a preferential monocyte transmigration for the diseased model [44]. Finally, vessel-on-a-chip models have been developed to study atherosclerosis using a microfluidic platform including an engineered architecture recapitulating the microphysiological environment and architectures of functional human organs, connected to a pump to control flow rates and shear stress [45,46].…”

Section: Discussionmentioning

confidence: 99%

The Interplay between Atherosclerosis and Cancer: Breast Cancer Cells Increase the Expression of Endothelial Cell Adhesion Markers

Scalia,

Doumani,

Kindt

et al. 2023

Biology

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Cardiovascular diseases are the leading causes of death worldwide, closely followed by cancer. To investigate the impact of breast cancer cell lines (SKBR3, MCF-7, and MDA-MB-231) on endothelial cell adhesion, a blended medium containing 30% breast-cancer-conditioned medium was prepared. This medium was then exposed to human umbilical vein endothelial cells (HUVECs) and monocytes (THP-1) for 48 h. Homemade oxidized low-density lipoproteins (oxLDL) were optionally added to the blended medium. Immunofluorescence was performed to assess the expression of E-selectin, connexin-43, and ICAM-1 on HUVECs, as well as LOX-1, CD36, and CD162 on THP-1. Additionally, unoxidized LDL was exposed to the three breast cancer cell lines for 48 h, and the formation of oxLDL was quantified. Our results revealed an upregulation of all six adhesion markers involved in the initiation of atherosclerosis when HUVECs and THP-1 were exposed to the breast-cancer-conditioned medium. Furthermore, this expression was further increased by exposure to oxLDL. We also observed a significant elevation in oxLDL levels when LDL was exposed to breast cancer cells. In conclusion, our findings successfully demonstrate an increased LDL oxidation in the presence of breast cancer cells, accompanied by an augmented expression of receptors involved in atherosclerosis initiation. These findings shed new light on the clinically observed interplay between atherosclerosis and cancer.

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Section: Discussionmentioning

confidence: 99%

The Interplay between Atherosclerosis and Cancer: Breast Cancer Cells Increase the Expression of Endothelial Cell Adhesion Markers

Scalia,

Doumani,

Kindt

et al. 2023

Biology

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“…The resultant TEBVs achieved similar mechanical properties to that of native arteries. Similarly, Lissy et al created 2-layered TEBVs by seeding ECs and SMCs on PCL conduit with controllable wall thickness and shear stress (162). In addition to the seeding strategy, cell sheet technology has also been used as an alternative approach.…”

Section: In Vitro 3d Vessel Based Systems Tissue-engineered Blood Vessels (Tebvs)mentioning

confidence: 99%

Recent Progress in in vitro Models for Atherosclerosis Studies

Zhang

Millican

Lynd

et al. 2022

Front. Cardiovasc. Med.

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Atherosclerosis is the primary cause of hardening and narrowing arteries, leading to cardiovascular disease accounting for the high mortality in the United States. For developing effective treatments for atherosclerosis, considerable efforts have been devoted to developing in vitro models. Compared to animal models, in vitro models can provide great opportunities to obtain data more efficiently, economically. Therefore, this review discusses the recent progress in in vitro models for atherosclerosis studies, including traditional two-dimensional (2D) systems cultured on the tissue culture plate, 2D cell sheets, and recently emerged microfluidic chip models with 2D culture. In addition, advanced in vitro three-dimensional models such as spheroids, cell-laden hydrogel constructs, tissue-engineered blood vessels, and vessel-on-a-chip will also be covered. Moreover, the functions of these models are also summarized along with model discussion. Lastly, the future perspectives of this field are discussed.

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