Potential of Trichoderma spp. Secondary Metabolite in Controlling Vascular Streak Dieback (VSD) on Cacao Seedlings

Harni, Rita; Amaria, Widi; Syafaruddin, Syafaruddin; Mahsunah, Anis Herliyati

doi:10.21082/jtidp.v4n2.2017.p57-66

Cited by 9 publications

(10 citation statements)

References 7 publications

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“…Vinale et al (2014) melaporkan bahwa kultur filtrat dari T. harzianum dapat menginduksi ketahanan tanaman tomat, serta menghambat pertumbuhan dari hifa Sclerotinia sclerotiorum dan Rhizoctonia solani. Harni, Amaria, Syafaruddin, & Mahsunah (2017) melaporkan metabolit sekunder T. amazonicum dan T. virens dapat menekan intensitas penyakit VSD pada bibit kakao.…”

Section: Pendahuluanunclassified

“…Isolat Trichoderma virens LP1 dan T. amazonicum LP3 yang digunakan merupakan isolat potensial yang telah diuji keefektifannya terhadap C. theobromae di laboratorium dan rumah kaca (Harni et al, 2017). T. virens LP1 dan T. amazonicum LP3 diremajakan dan diperbanyak pada medium potato dextrose agar (PDA).…”

Section: Perbanyakan Massal Metabolit Sekunder Trichoderma Sppunclassified

“…Starter dibuat dengan cara menambahkan 2 ml larutan NaCl fisiologis steril ke dalam agar miring (10 ml) Trichoderma spp. Selanjutnya, 1 ml suspensi [1% (v/v)] dimasukkan ke dalam erlenmeyer 500 ml yang telah berisi media tahap vegetatif 100 ml, dan diinkubasi pada suhu 28°C menggunakan orbital shaker (200 rpm; 48 jam) (Harni et al, 2017).…”

Section: Perbanyakan Massal Metabolit Sekunder Trichoderma Sppunclassified

“…Dengan demikian, dapat memberikan pengaruh positif terhadap pertumbuhan dan produksi tanaman. Sejalan dengan hasil penelitian sebelumnya yang dilakukan oleh Harni et al (2017) bahwa T. virens LP1 dan T. amazonicum LP3 dapat menekan keparahan penyakit VSD pada bibit kakao masing-masing sebesar 81,80% dan 63,20% dan dapat memicu pertumbuhan benih kakao.…”

Section: Pengaruh Metabolit Sekunder Dan Fungisida Nabati Terhadap Prunclassified

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Pengaruh Metabolit Sekunder Trichoderma spp. dan Fungisida Nabati untuk Mengendalikan Penyakit VSD pada Tanaman Kakao

Harni

Amaria

Mahsunah

et al. 2019

J. tanam. industri dan penyegar

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Vascular streak dieback (VSD) caused by Ceratobasidium theobromae is a major disease in cacao which results in yield losses up to 45%. Controlling VSD using secondary metabolites and botanical fungicide is expected to reduce disease attacks because secondary metabolites contain antibiotics, enzymes, and toxins that can control the disease and botanical fungicide that are antifungal. The research aimed to determine the effect of Trichoderma spp. secondary metabolites and botanical fungicide to control VSD in cacao. The research was conducted in the Integrated Laboratory, IIBCRI, Sukabumi, Biotechnology Laboratory, BPPT, Serpong and farmer gardens in Rahmat Village, Palolo District, Sigi Regency, Central Sulawesi, from March to December 2017. The research used a randomized block design of 7 treatments with 3 replications; each replication consists of 10 plants. The treatments were: (1) Trichoderma virens LP1, (2) T. amazonicum LP3, (3) botanical fungicide, (4) T. virens LP1 + botanical fungicide, (5) T. amazonicum LP3 + botanical fungicide, (6) chemical fungicide (for comparison), (7) control (without application). Variables observed were the symptoms of the attack, severity and disease progression, and production. The results showed that Trichoderma spp. secondary metabolites suppress VSD disease severity and increase cacao production up to 33.97%–61.34%, either in a single form or in combination with botanical fungicide. T. virens LP1 secondary metabolite showed the highest suppression of VSD disease (54.18%), followed by the combination of T. virens LP1 + botanical fungicide (47.64%), better than chemical fungicide (30.89%). Thus, Trichoderma spp. secondary metabolites can be used as a VSD control technology.

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Section: Pendahuluanunclassified

Section: Perbanyakan Massal Metabolit Sekunder Trichoderma Sppunclassified

Section: Pengaruh Metabolit Sekunder Dan Fungisida Nabati Terhadap Prunclassified

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Pengaruh Metabolit Sekunder Trichoderma spp. dan Fungisida Nabati untuk Mengendalikan Penyakit VSD pada Tanaman Kakao

Harni

Amaria

Mahsunah

et al. 2019

J. tanam. industri dan penyegar

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“…Metabolit sekunder yang dihasilkan oleh T. virens dan T. amazonicum juga telah diaplikasikan pada bibit kakao, dengan penekanan intensitas penyakit VSD masingmasing sebesar 63,2% dan 81,8% (Harni, Amaria, Syafaruddin, & Mahsunah, 2017).…”

Section: Pengaruh Biofungisida Trichoderma Spp Terhadap Perkembanganunclassified

Pengaruh Dosis dan Frekuensi Aplikasi Biofungisida Trichoderma terhadap Infeksi Rigidoporus microporus pada Benih Karet

Amaria

Harni

Wardiana

2018

J. tanam. industri dan penyegar

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Biological agents Trichoderma virens and T. amazonicum have been developed and examined for their effectiveness through in vitro and in vivo approaches against Rigidoporus microporus, the cause of white root disease (WRD) in rubber. The effectiveness of these bio-agents can be determined by testing the dosage and frequency of Trichoderma spp. biofungicide application. The research aimed to investigate the effective dose and application frequency of Trichoderma spp. biofungicide on R. microporus infection in rubber seedling. The experiment was conducted in laboratory and screen house of Indonesian Industrial and Beverage Crops Research Institute (IIBCRI), Sukabumi, from June to December 2014. A randomized block design was used with 14 treatments and 3 replications, i.e biofungicide combination (T. virens and T. amazonicum), dosage (25, 50, and 75 g), application frequencies (1 and 2 times application), and two controls (positive and negative). Rubber seedlings used were propellegitiem seeds of GT1 clone planted in polybags. Trichoderma spp. was multiplied using fermentation method in liquid medium, whereas biofungicide was formulated using talc as carrier. Observed variables including Trichoderma spp. population number, incubation period, attack intensity, and WRD attack suppression. The results showed that T. virens and T. amazonicum biofungicides with 50 g/plant dose at one application was the most effective and efficient in suppressing R. microporus development on rubber seedlings. The type, dosage, and frequencies of application increased Trichoderma spp. population in soil, prolonged the pathogen’s incubation period, decreased WRD attack intensity, and suppress the attack of WRD disease.

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The potency of secondary metabolites endophytic fungi Trichoderma sp as biocontrol of Colletotrichum sp and Fusarium oxysporum causing disease in chili

Utami¹,

Nisa²,

Putri³

et al. 2019

International Conference on Biology and Applied Science (Icobas)

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Potential of Trichoderma spp. Secondary Metabolite in Controlling Vascular Streak Dieback (VSD) on Cacao Seedlings

Cited by 9 publications

References 7 publications

Pengaruh Metabolit Sekunder Trichoderma spp. dan Fungisida Nabati untuk Mengendalikan Penyakit VSD pada Tanaman Kakao

Pengaruh Metabolit Sekunder Trichoderma spp. dan Fungisida Nabati untuk Mengendalikan Penyakit VSD pada Tanaman Kakao

Pengaruh Dosis dan Frekuensi Aplikasi Biofungisida Trichoderma terhadap Infeksi Rigidoporus microporus pada Benih Karet

The potency of secondary metabolites endophytic fungi Trichoderma sp as biocontrol of Colletotrichum sp and Fusarium oxysporum causing disease in chili

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