Potential Predictive Role of MicroRNAs in the Neoadjuvant Treatment of Esophageal Cancer

Vrána, David; Matzenauer, Marcel; Aujeský, R; Vrba, Radek; Neoral, C; Melichar, Bohuslav; Souček, Pavel

doi:10.21873/anticanres.11332

Cited by 14 publications

(16 citation statements)

References 49 publications

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“…36 At the molecular biology level, the genetic susceptibility of esophageal cancer is related to the activation and expression of oncogenes and the loss or inactivation of tumor suppressor genes. 19,33 At present, the specific pathogenesis of esophageal cancer is still unclear. Molecular biology research on esophageal cancer, searching for early diagnostic markers of esophageal cancer and related factors and mechanisms of invasion and metastasis have become a hot topic in this field.…”

Section: Discussionmentioning

confidence: 99%

<p>HIPK3 Promotes Growth and Metastasis of Esophageal Squamous Cell Carcinoma via Regulation of miR-599/c-MYC Axis</p>

Ba¹,

Liu²,

Li³

et al. 2020

OTT

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Background/Aims: this experimental design was based on HIPK3 to explore the pathogenesis of ESCC. Methods: RT-qPCR was used to detect the expression of CircHIPK3 and miR-599 in ESCC tissues and cell lines.CCK-8, colony formation, flow cytometry and transwell assay were used to detect the effects of CircHIPK3 and miR-599 on tumor cell proliferation, apoptosis and migration and invasion. Target gene prediction and screening, luciferase reporter assays were used to validate downstream target genes of CircHIPK3 and miR-599.mRNA and protein expression of c-MYC were detected by RT-qPCR and Western blotting. The tumor changes in mice were detected by in vivo experiments in nude mice. Results: HIPK3 was highly expressed in ESCC tissues and cell lines. In addition, HIPK3 expression levels were associated with advanced TNM stage, lymph node metastasis and tumor size. Moreover, HIPK3 was significantly promoted cell proliferation and migration of ESCC cells. In addition, HIPK3 was able to inhibit miRNA-599 expression and up-regulate the expression level of c-MYC. Finally, the results of in vivo animal models confirmed that HIPK3 promoted ESCC progression by modulating the miR-599/c-MYC axis. Conclusion: HIPK3 can regulate the proliferation of esophageal squamous cell carcinoma cells by regulating miR-599/c-MYC axis, thereby inhibiting the occurrence and development of esophageal squamous cell carcinoma.

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Section: Discussionmentioning

confidence: 99%

<p>HIPK3 Promotes Growth and Metastasis of Esophageal Squamous Cell Carcinoma via Regulation of miR-599/c-MYC Axis</p>

Ba¹,

Liu²,

Li³

et al. 2020

OTT

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“…miRNAs have been recognized as a new class of genes involved in human cancer and have recently been shown to be biomarkers of diagnostic, prognostic and therapeutic (10,11). Several studies have identified some miRNAs that were significantly altered and have the potential to restrain proliferation of cervical cancer (12,13). Previous studies suggest that miRNA-138 restrains cervical cancer cells proliferation (14).…”

Section: Introductionmentioning

confidence: 99%

Long non-coding RNA BCYRN1 promotes the proliferation and metastasis of cervical cancer via targeting microRNA-138 inï¿½vitro and inï¿½vivo

et al. 2018

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Abstract. Cervical cancer is one of the most malignant types of tumor and the fourth leading cause of cancer-associated mortality in females worldwide. High expression of brain cytoplasmic RNA 1 (BCYRN1) has been detected in various tumors. The present study aimed to investigate the effect of BCYRN1 in the viability and motility of cervical cancer, and the relevant mechanism. The results demonstrated that BCYRN1 was upregulated in cervical cancer tissues compared with normal tissues. Elevated levels of BCYRN1 were also detected in three human cervical cancer cell lines (SiHa, HeLa and CaSki) compared with non-cancerous ectocervical epithelial cell line (Ect1/E6E7). The expression of BCYRN1 was suppressed following transfection with small interfering RNA (siRNA) in HeLa cells. The silence of BCYRN1 significantly reduced cell viability and motility. Furthermore, microRNA (miR)-138 was predicted as a direct target of BCYRN1 and the expression of miR-138 was elevated in HeLa cells transfected with BCYRN1 siRNA. Subsequently, elevated levels of miR-138 were suppressed by transfection with miR-138 inhibitor in HeLa cells pretreated with BCYRN1 siRNA. The targeting association between BCYRN1 and miR-138 was supported by luciferase reporter assays. Additionally, BCYRN1 siRNA partially counteracted the effect of miR-138 inhibitor on promoting cell viability and mobility in HeLa cells. Finally, the in vivo experiment verified that BCYRN1 siRNA was able to prevent tumor growth, and reduced the expression of migration marker proteins metalloproteinase 2 and vascular endothelial cell growth factor, with enhanced expression levels of miR-138. These results suggest that lncRNA BCYRN1 promotes the proliferation and invasion of cervical cancer via targeting miR-138.

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“…Paralysis of the left reccurent laryngeal nerve in patients operated on using the cervical approach is reported in up to 14% of patients 35 . There is a consensus that is based on the analysis of postoperative results that surgery for esophageal cancer should be performed in high volume centres 36, however despite this effort and intensive research in this field [37][38][39] the general outcome of the treatment remains unsatisfactory. The present retrospective analysis has some obvious limitations.…”

Section: Discussionmentioning

confidence: 99%

Complications of the surgical treatment of esophageal cancer and microbiological analysis of the respiratory tract

Vrba¹,

Neoral²,

Vomáčková³

et al. 2020

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub

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The aim of this study was to reduce the severe respiratory complications of esophageal cancer surgery often leading to death. Methods. Two groups of patients operated on for esophageal cancer were evaluated in this retrospective analysis. The first group was operated between 2006-2011, prior to the implementation of preoperative microbiological examination while the second group had surgery between 2012-2017 after implementation of this examination. Results. In total, 260 patients, 220 males and 40 females underwent esophagectomy. Between 2006-2011, 113 (87.6%) males and 16 (12.4%) females and between 2012-2017, esophagectomy was performed in 107 (81.7%) males and 24 (18.3%) females. In the first cohort, 10 patients died due to respiratory complications. The 30-day mortality was 6.9% and 90-day was 9.3%. In the second cohort, 4 patients died from respiratory complications. The 30-day mortality was 1.5% and 90-day mortality was 3.1%. With regard to the incidence of respiratory complications (P=0.014), these occurred more frequently in patients with sputum collection, however, severe respiratory complications were more often observed in patients without sputum collection. Significantly fewer patients died (P=0.036) in the group with sputum collection. The incidence of respiratory complications was very significantly higher in the patients who died (P<0.0001). Conclusion. The incidence of severe respiratory complications (causing death) may be reduced by identifying clinically silent respiratory tract infections.

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Potential Predictive Role of MicroRNAs in the Neoadjuvant Treatment of Esophageal Cancer

Cited by 14 publications

References 49 publications

<p>HIPK3 Promotes Growth and Metastasis of Esophageal Squamous Cell Carcinoma via Regulation of miR-599/c-MYC Axis</p>

<p>HIPK3 Promotes Growth and Metastasis of Esophageal Squamous Cell Carcinoma via Regulation of miR-599/c-MYC Axis</p>

Long non-coding RNA BCYRN1 promotes the proliferation and metastasis of cervical cancer via targeting microRNA-138 inï¿½vitro and inï¿½vivo

Complications of the surgical treatment of esophageal cancer and microbiological analysis of the respiratory tract

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