Potentially human‐virulentVibrio vulnificusisolates from diseased great pompano (Trachinotus goodei)

Abstract

Summary Vibrio vulnificus is an opportunistic human pathogen responsible for the majority of seafood‐associated deaths worldwide and is also a relevant fish pathogen for the aquaculture industry. In addition to infections in aquatic livestock, V. vulnificus also represents a risk to aquarium animals. For the first time, this work describes an important mortality outbreak in Trachinotus goodei in a zoo aquarium, with the isolation of Vibrio vulnificus (Vv) from the internal organs of the diseased fish. The isolates were identified by MALDI‐TOF MS, serotyped and characterized by pulsed‐field gel electrophoresis (PFGE). Although the isolates from great pompanos did not belong to pathovar piscis (formerly biotype 2) or to any of the fish‐related serovars, they all had identical phenotypes, antimicrobial susceptibility profiles and PFGE patterns, which together with their isolation in pure culture from internal organs is strongly indicative of their clinical significance. Moreover, Vv isolates harboured important genetic markers of human virulence potential: they had the clinical variant of the vcg gene, gave the 338 bp DNA amplification product of the pilF gene and resisted the bactericidal activity of human serum. All these results strongly suggest that these Vv isolates should be considered potentially virulent for humans. These results extend the range of fish species affected by V. vulnificus, confirm the threat that this pathogen represents to aquatic animals and highlight the risk that this bacterial pathogen poses to human health.

Nutritional Evaluation of Black Soldier Fly Frass as an Ingredient in Florida Pompano (Trachinotus carolinus L.) Diets

“…The increase in Vibrio, one of the most common genera in marine carnivorous fish [ 53 , 61 ], was especially pronounced in the 6% frass group, followed by the 18% and 12% frass inclusion groups ( Figure 3 c). Some Vibrio species have been reported to exhibit probiotic features by assisting in the degradation of dietary materials in the fish gut [ 61 ], while other species are known as opportunistic fish pathogens [ 62 , 63 ]. Although the fiber contents were altered among the different diets, there was not a large difference among the bacterial species known to metabolize fiber.…”

Genomic and Phenotypic Characteristics for Vibrio vulnificus Infections

“… 8 Epidemiological investigations showed an overlap in the distribution of VV among marine animals, aquariums, and human. 9 , 10 However, the genetic factors that differentiate clinical or environmental isolates remain enigmatic. Pangenome analysis has identified VV isolates cohabitated with both commensal and bloomer ecotypes in the mucosa of eels from aquaculture farms, raising concerns about man-made environments.…”