Practical Hints on Absorption Spectrometry

Edisbury, J. R.

doi:10.1007/978-1-4899-6401-4

Cited by 28 publications

(19 citation statements)

References 19 publications

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“…Aqueous solutions of potassium nitrate exhibit a nearly symmetrical absorption peak with Amax at 302 nm. This system has been studied extensively by Vandenbelt [14] and by the British Photoelectric Spectrometry Group in two collaborative tests [28,29]. Molar absorptivities reported vary from 7.06 to 7.20 liter mol-1 cm-I • This range of values undoubtedly results in part from the fact that KN03 solutions deviate significantly from Beer's law.…”

Section: Resultsmentioning

confidence: 99%

Liquid absorbance standards

Burke

Deardorff

Menis

1972

J. RES. NATL. BUR. STAN. SECT. A.

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Errors in the meas ureme nt of the absorbances of liquid filters res ult from instrumental and c hemical un ce rtainties. This paper presents a systematic study of these variables on the absorbances of selected filters. Three types of liquid filters are di sc ussed. These are (1) individual solutions of hi gh purity compounds, (2) composi te mixtures and (3) aqueous solutions of organic dyes. The accuracy of th e absorptivity data is established using NBS-calibrated glass filters. The magnitude of the errors ari sing from spectral bandpass, beam geometry, stray light, internal multipl e refl ections, and refractiv e index are delin eated. Finally, as a practical outgrowth of this study, th e development and iss uan ce of NBS Standard Refere nce Material 931, Liquid Absorbance Standards for Ultraviolet and Visible Spectrophotometry, is described.

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Section: Resultsmentioning

confidence: 99%

Liquid absorbance standards

Burke

Deardorff

Menis

1972

J. RES. NATL. BUR. STAN. SECT. A.

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“…The sample loop volume was determined by a spectrophotometric method using basic chromate solutions which are known to be suitable as absorption standards [8]. The method consisted of measuring the absorbance at 373 nm of three loopfuls of an aqueous stock solution of K 2 Cr0 4 (1.3% by weight) diluted to 50 mL with 0.2 percent KOH, and measuring the absorbance of the same stock solution after diluting 1:500 with 0.2 percent KOH.…”

Section: Hplc Systemmentioning

confidence: 99%

Generator Columns and High Pressure Liquid Chromatography for Determining Aqueous Solubilities and Octanol-Water Partition Coefficients of Hydrophobic Substances

DeVoe¹,

Miller²,

Wasik³

1981

J. RES. NATL. BUR. STAN.

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Generator columns packed with a solid support and loaded with a liquid organic phase make it possible to rapidly and conveniently equilibrate water with the organic phase. By coupling the generator column to an extractor column for high pressure liquid chromatographic analysis of the aqueous solution, errors from surface adsorption and loss to the atmosphere are avoided. Using this method, the mean values and confidence limits at a 95 percent confidence level of the aqueous solubility, S, and the octanol-water partition coefficient, P, of n-propylbenzene at 25°C were found to be S = (4.32 ± 0.02) X 1O-4 M and log P = 3.720 ± 0.003.

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“…Interlaboratory surveys for this evaluation in (clinical) chemistry laboratories have been performed only on a very limited scale. These surveys are usually restricted to the measurement of the absorbances of dichromate or cobaltous sulphate Solutions (1)(2)(3)(4). In the programmes of the German Society for Clinical Chemistry, absorbance checks at different wavelength are performed at regulär intervals.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous Spectrophotometric Calibration of Wavelength and Absorbance in an Interlaboratory Survey Using Holmium Oxide (HO2O3) in Perchloric Acid as Reference, Compared with p-Nitrophenol and Cobaltous Sulphate Solutions (1978-1984)

Jansen¹,

Kampen²,

Steigstra³

et al. 1986

Clinical Chemistry and Laboratory Medicine

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Summary:The wavelength accuracy of ten different types of spectrophotometer was tested with holmium perchlorate solutions. It was found to be good, with mean deviations from the literature values of maximally 0.3 nm. Standard deviations over the entire spectral ränge were within 0.75 nm.The absorbance accuracy for different types of Instruments was generally within 5%, except in the 287 nm ränge where higher deviations were found. The sharpness of the holmium peaks, in combination with band width and sensitivity of the Instruments, troubled the majority of the participants. 150 spectrophotometers ! were involved in the surveys. ! « Linearity of the spectrophotometers was tested with /7-nitrophenol and cobaltous sulphate and found to be j satisfactory.

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Practical Hints on Absorption Spectrometry

Cited by 28 publications

References 19 publications

Liquid absorbance standards

Liquid absorbance standards

Generator Columns and High Pressure Liquid Chromatography for Determining Aqueous Solubilities and Octanol-Water Partition Coefficients of Hydrophobic Substances

Simultaneous Spectrophotometric Calibration of Wavelength and Absorbance in an Interlaboratory Survey Using Holmium Oxide (HO2O3) in Perchloric Acid as Reference, Compared with p-Nitrophenol and Cobaltous Sulphate Solutions (1978-1984)

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