Pre-adsorbed type-I collagen structure-dependent changes in osteoblastic phenotype

Hanagata, Nobutaka; Takemura, Tamiko; Monkawa, Akira; Ikoma, Toshiyuki; Tanaka, Junzo

doi:10.1016/j.bbrc.2006.04.021

Cited by 23 publications

(37 citation statements)

References 35 publications

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“…As a result, various phenotype and gene expression patterns were found to be different from cells on PS dishes. 16,17 Cell functions are determined not only by interfacial proteins but also by substrate surface properties. An understanding of cell adhesion and spreading processes on interfacial protein layers adsorbed on a substrate surface is of significant importance.…”

Section: Protein-mediated Cell Adsorption Formation Of Nano-bio Intermentioning

confidence: 99%

“…Surface morphology at the nano-and micrometer scales and subsequent protein adsorption are known to affect later cellular activities (proliferation, survival and gene expression). [15][16][17] The later activities are significantly affected by initial cellular activities (adhesion, spreading). Thus, to evaluate whether the surfaces of engineered biomaterials can induce desirable initial cell interactions, in vitro cell culture is performed because there are no applicable universal basic rules to predict cell behaviors on the basis of certain material surface properties.…”

Section: Introductionmentioning

confidence: 99%

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In situ QCM-D study of nano-bio interfaces with enhanced biocompatibility

Tagaya

2015

Polym J

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When biomaterials are implanted into an animal body, the body fluid proteins initially adsorb and then cells recognize these surfaces. Adherent cell functions respond differently to diverse biomaterial surfaces with different properties. Thus, an understanding of cellular responses to biomaterials is crucial for effective control of biomaterial − cell interactions. I have researched how to clarify interfacial phenomena via protein adsorption and subsequent cell adhesion to hydroxyapatite nanocrystals using a quartz crystal microbalance with a dissipation technique. In this review, I focused on the current understanding of enhanced biocompatibility by exploring the roles of protein mediation at the interface. The most promising nano-bio interfaces are explained, and different protein adsorption and cell adhesion processes are highlighted depending on their interfacial states. This approach will clarify several ambiguities of interfacial phenomena between biomaterials and cells and will help in the design of novel biomaterials that can be implanted.

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Section: Protein-mediated Cell Adsorption Formation Of Nano-bio Intermentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

In situ QCM-D study of nano-bio interfaces with enhanced biocompatibility

Tagaya

2015

Polym J

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“…The ECM regulates the function of cells and drives cellular differentiation. Thus ECMs show bioactivities including inducing proliferation and differentiation [2,5,8,9]. The in vitro bioactivities of many synthetic and natural ECMs are not fully understood.…”

Section: Introductionmentioning

confidence: 99%

Fibroblast-Derived Extracellular Matrix Induces Chondrogenic Differentiation in Human Adipose-Derived Mesenchymal Stromal/Stem Cells in Vitro

Dzobo

Turnley

Wishart

et al. 2016

IJMS

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Mesenchymal stromal/stem cells (MSCs) represent an area being intensively researched for tissue engineering and regenerative medicine applications. MSCs may provide the opportunity to treat diseases and injuries that currently have limited therapeutic options, as well as enhance present strategies for tissue repair. The cellular environment has a significant role in cellular development and differentiation through cell–matrix interactions. The aim of this study was to investigate the behavior of adipose-derived MSCs (ad-MSCs) in the context of a cell-derived matrix so as to model the in vivo physiological microenvironment. The fibroblast-derived extracellular matrix (fd-ECM) did not affect ad-MSC morphology, but reduced ad-MSC proliferation. Ad-MSCs cultured on fd-ECM displayed decreased expression of integrins α2 and β1 and subsequently lost their multipotency over time, as shown by the decrease in CD44, Octamer-binding transcription factor 4 (OCT4), SOX2, and NANOG gene expression. The fd-ECM induced chondrogenic differentiation in ad-MSCs compared to control ad-MSCs. Loss of function studies, through the use of siRNA and a mutant Notch1 construct, revealed that ECM-mediated ad-MSCs chondrogenesis requires Notch1 and β-catenin signaling. The fd-ECM also showed anti-senescence effects on ad-MSCs. The fd-ECM is a promising approach for inducing chondrogenesis in ad-MSCs and chondrogenic differentiated ad-MSCs could be used in stem cell therapy procedures.

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“…No significant difference in cell number, ALP activity and gene expression of three osteoblast-related proteins was found. However, a previous study by Hanagata et al (2006) showed that proliferation and mineralization were delayed on the fibrillar form of collagen. The discrepancy between these two studies might be due to the investigation at different stages of osteoblastic maturation.…”

Section: Resultsmentioning

confidence: 86%

“…Therefore, osteoblast behavior might be modulated by collagen structure. Previously, Hanagata et al (2006) studied the effects of two different structures of type I collagen (acid-soluble and fibrillar) on the late stage of maturation process of osteoblast-like cells. They found that proliferation and mineralization were delayed on the fibrillar form of collagen, suggesting that collagen structure affects osteoblast-like cells.…”

Section: Introductionmentioning

confidence: 99%