Pre- and post-natal muscle microRNA expression profiles of two pig breeds differing in muscularity

Siengdee, Puntita; Trakooljul, Nares; Muráni, Eduard; Brand, Bodo; Schwerin, Manfred; Wimmers, Klaus; Ponsuksili, Siriluck

doi:10.1016/j.gene.2015.02.035

Cited by 25 publications

(28 citation statements)

References 61 publications

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“…A lecture showed that the inhibition of miR-29 by TGF-beta-Smad3 signaling through dual mechanisms promotes the trans-differentiation of mouse myoblasts into myofibroblasts 23 . In agreement with a previous study, miR-29a was also increased in abundance after birth 13 . These results indicated these miRNAs play important roles in muscle hypertrophy.…”

Section: Discussionsupporting

confidence: 93%

“…Thus, it can be reasonably speculated that in LR and LT, these known myogenic genes may be distinctly expressed to regulate myofibrillar genes in muscle development during the embryonic stages and muscle hypertrophy during the postnatal stages. A recent study comparing pre- and post-natal muscle microRNA expression profiles in the longissimus dorsi muscle of LR and Pietrain pigs also revealed that several pathways related to muscle development were enriched with both the predicted targets of the differentially expressed miRNAs and the miRNA with breed and/or stage differences identified in the present study 13 . Those findings further indicate that miRNAs could play key roles in the phenotypic variations of porcine muscles.…”

Section: Discussionsupporting

confidence: 73%

“…Moreover, numerous miRNAs have been shown to be associated with skeletal muscle development. A recent study compared the pre- and post-natal microRNA expression profiles in the longissimus dorsi muscles of LR and Pietrain pigs, as the muscularity of this muscle differs between these breeds; those authors found that the dynamic expression and breed-associated regulation of porcine muscle miRNAs suggests a functional role for miRNA-mediated gene regulation during muscle development and in establishing phenotypic variations of muscle traits 13 . Our previous research discovered 18 novel candidate myogenic miRNAs in LR during muscle development 2 .…”

Section: Introductionmentioning

confidence: 99%

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An integrated analysis revealed different microRNA-mRNA profiles during skeletal muscle development between Landrace and Lantang pigs

Xie

Chen

Zhang

et al. 2017

Sci Rep

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Pigs supply vital dietary proteins for human consumption, and their economic value depends largely on muscle production. MicroRNAs are known to play important roles in skeletal muscle development. However, their relationship to distinct muscle production between pig breeds remains unknown. Here, we performed an integrated analysis of microRNA-mRNA expression profiles for Landrace (LR, lean) pigs and the Chinese indigenous Lantang pig (LT, lard-type) during 8 stages of skeletal muscle developmental, including at 35, 49, 63, 77 dpc (days post coitum) and 2, 28, 90, 180 dpn (days postnatal). As differentially expressed-miRNA expression profiles can be well classified into two clusters by PCA analysis, we grouped the embryonic stages as G1 and the postnatal stages as G2. A total of 203 genes were predicted miRNA targets, and a STEM analysis showed distinct expression patterns between G1 and G2 in both breeds based on their transcriptomic data. Furthermore, a STRING analysis predicted interactions between 22 genes and 35 miRNAs, including some crucial myogenic factors and myofibrillar genes. Thus, it can be reasonably speculated that myogenic miRNAs may regulate myofibrillar genes in myofiber formation during embryonic stages and muscle hypertrophy during postnatal stages, leading to distinct differences in muscle production between breeds.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionsupporting

confidence: 73%

Section: Introductionmentioning

confidence: 99%

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An integrated analysis revealed different microRNA-mRNA profiles during skeletal muscle development between Landrace and Lantang pigs

Xie

Chen

Zhang

et al. 2017

Sci Rep

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“…Many miRNAs were differentially expressed in mammary gland of lactating Jinhua versus Yorkshire breeds of swine21, whereas several miRNAs expressed in the longissimus dorsi muscle varied between German Landrace and Pietrain pig breeds22. Expression of 125 miRNAs in kidney varied among pig breeds, including the Iberian breed, European Wild Boar ancestor, Landrace, Large White, Piétrain, Meishan and Vietnamese breeds10.…”

Section: Resultsmentioning

confidence: 99%

MicroRNA in sperm from Duroc, Landrace and Yorkshire boars

Kasimanickam

Kastelic

2016

Sci Rep

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Sperm contain microRNAs (miRNAs), which may have roles in epigenetic control. Regarding phylogenetic relationships among various swine breeds, Yorkshire and Landrace, are considered phenotypically and genetically very similar, but distinctly different from Duroc. The objective of the present study was to compare abundance of boar sperm miRNAs in these three breeds. Overall, 252 prioritized miRNAs were investigated using real-time PCR; relative expression of miRNAs in sperm was similar in Yorkshire and Landrace boars, but significantly different compared to Duroc. Seventeen miRNAs (hsa-miR-196a-5p, hsa-miR-514a-3p, hsa-miR-938, hsa-miR-372-3p, hsa-miR-558, hsa-miR-579-3p, hsa-miR-595, hsa-miR-648, hsa-miR-524-3p, hsa-miR-512-3p, hsa-miR-429, hsa-miR-639, hsa-miR-551a, hsa-miR-624-5p, hsa-miR-585-3p, hsa-miR-508-3p and hsa-miR-626) were down-regulated (P < 0.05; fold regulation ≤−2) in Yorkshire and Landrace sperm, compared to Duroc sperm. Furthermore, three miRNAs (hsa-miR-9-5p, hsa-miR-150-5p, and hsa-miR-99a-5p) were significantly up-regulated in Yorkshire and Landrace sperm compared to Duroc sperm, However, 240 miRNAs were not significantly different (within + 2 fold) between Yorkshire and Landrace sperm. We concluded that miRNAs in sperm were not significantly different between Yorkshire and Landrace boars, but there were significant differences between those two breeds and Duroc boars. Furthermore, integrated target genes for selected down-regulated miRNAs (identified via an in-silico method) appeared to participate in spermatogenesis and sperm functions.

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“…This applies in particular to pigs as well as beef cattle (different miRNA expression in muscle tissue in different breeds) and dairy cattle (miRNAs in muscle, mammary gland) (51,62). All these studies indicate that the high developmental potential of the muscular tissue and mammary gland in dairy/meat cattle, leading to high milk/meat productivity, also depends on a specific miRNA expression pattern.…”

Section: Biogenesismentioning

confidence: 92%

MicroRNA function in domestic animal physiology and diseases: a promising diagnostic tool for veterinary use

Chodkowska¹,

Sadkowski²,

Ostaszewski³

2017

Medycyna Weterynaryjna

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SummaryMicroRNAs (miRNAs) are small non-coding interfering RNA molecules capable of post-transcriptionally regulating gene expression through sequence-specific base pairing to messenger ribonucleic acid (mRNA). In recent years, hundreds of miRNAs have been identified in humans, various animals and plants. The action of miRNAs has been examined in several biological processes, including tissue morphogenesis, development, cell proliferation and differentiation, apoptosis, immunity, metabolism, and major signaling pathways. Changes in miRNA expression have also been analyzed in the context of various pathological conditions, including different kinds of inflammation, cancer, cardiovascular diseases, etc. Thanks to these investigations, several miRNAs have been identified as potential sensitive diagnostic markers that may be important in monitoring physiological and pathological processes. In human medicine, microarray and real-time PCR-based diagnostic test panels with selected disease-specific miRNAs are increasingly used to predict disease occurrence or progression. In animals, they are mainly used to diagnose canine mammary cancers and infectious diseases, as well as to monitor reproduction. Until now, only few miRNAs of domestic animals have been studied in detail. Moreover, the silencing of selected miRNAs, successfully used in human medicine in diseases related to miRNA over-expression, is also emerging as a promising tool for veterinary medicine and animal breeding.This review presents recent progress in miRNA biology in various domestic animals and shows the current state of knowledge concerning miRNAs and their potential role as a diagnostic factor in veterinary sciences.

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Pre- and post-natal muscle microRNA expression profiles of two pig breeds differing in muscularity

Cited by 25 publications

References 61 publications

An integrated analysis revealed different microRNA-mRNA profiles during skeletal muscle development between Landrace and Lantang pigs

An integrated analysis revealed different microRNA-mRNA profiles during skeletal muscle development between Landrace and Lantang pigs

MicroRNA in sperm from Duroc, Landrace and Yorkshire boars

MicroRNA function in domestic animal physiology and diseases: a promising diagnostic tool for veterinary use

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