Pre-labelled oligo probe-FISH karyotype analyses of four Araliaceae species using rDNA and telomeric repeat

Zhou, Hui; Pellerin, Remnyl Joyce; Waminal, Nomar Espinosa; Yang, Tae-Jin; Kim, Hyun Hee

doi:10.1007/s13258-019-00786-x

Cited by 14 publications

(10 citation statements)

References 75 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…ex A.Murr.) Koidz [ 51 ], Ginkgo biloba L., Hordeum vulgare L., Phaseolus vulgaris sensu Blanco, non L. and Trigonella foenum - graecum L. [ 52 ], Rosa wichurana Cr‚p. [ 53 ], Cestrum elegans (Brongn.…”

Section: Discussionmentioning

confidence: 99%

“…5S rDNA has been used extensively as a chromosome marker and exhibits substantial conservation and stability in woody plants Annona cherimola L. [ 61 ], C. sinensis × P. trifoliata [ 23 ], A. elata , D. morbiferus , E. sessiliflorus , K. septemlobus [ 51 ], Ch. campanulatus [ 30 ], G. biloba and P. densiflora [ 52 ], H. rhamnoides [ 17 ], R. wichurana [ 53 ], Passiflora species [ 62 ], Cestrum species [ 56 ], and V. foetida [ 57 ].…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Oligo-FISH Can Identify Chromosomes and Distinguish Hippophaë rhamnoides L. Taxa

Luo

Liu

2022

Genes

View full text Add to dashboard Cite

Oligo-fluorescence in situ hybridization (FISH) facilitates precise chromosome identification and comparative cytogenetic analysis. Detection of autosomal chromosomes of Hippophaë rhamnoides has not been achieved using oligonucleotide sequences. Here, the chromosomes of five H. rhamnoides taxa in the mitotic metaphase and mitotic metaphase to anaphase were detected using the oligo-FISH probes (AG3T3)3, 5S rDNA, and (TTG)6. In total, 24 small chromosomes were clearly observed in the mitotic metaphase (0.89–3.03 μm), whereas 24–48 small chromosomes were observed in the mitotic metaphase to anaphase (0.94–3.10 μm). The signal number and intensity of (AG3T3)3, 5S rDNA, and (TTG)6 in the mitotic metaphase to anaphase chromosomes were nearly consistent with those in the mitotic metaphase chromosomes when the two split chromosomes were integrated as one unit. Of note, 14 chromosomes (there is a high chance that sex chromosomes are included) were exclusively identified by (AG3T3)3, 5S rDNA, and (TTG)6. The other 10 also showed a terminal signal with (AG3T3)3. Moreover, these oligo-probes were able to distinguish one wild H. rhamnoides taxon from four H. rhamnoides taxa. These chromosome identification and taxa differentiation data will help in elucidating visual and elaborate physical mapping and guide breeders’ utilization of wild resources of H. rhamnoides.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Oligo-FISH Can Identify Chromosomes and Distinguish Hippophaë rhamnoides L. Taxa

Luo

Liu

2022

Genes

View full text Add to dashboard Cite

show abstract

“…The Arabidopsis -type telomeric repeat sequences T 3 AG 3 are most like AG 3 T 3 , which are still the most frequent even in woody plants—such as species of Abies [ 37 ], Aralia [ 41 ], Cycas [ 39 ], Dendropanax [ 41 ], Eleutherococcus [ 41 ], Kalopanax [ 41 ], Malus [ 23 ], Picea [ 23 ], Pinus [ 23 , 40 , 42 , 43 , 44 ], Populus [ 35 ], Podocarpus [ 46 ], and Zamia [ 23 , 61 ]—but has been shown to be absent in species of Cestrum , Sessea , and Vestia [ 14 ]. Nevertheless, these sequences cannot be entirely equated, due to the existence of at least ten variable telomere sequences (T x A y G z ) n —including TAG 3 [ 4 ], TA 2 G 2 [ 5 ], T 2 AG 2 [ 6 ], T 2 AG 3 [ 7 ], TA 2 G 3 [ 5 ], T 3 AG 3 [ 8 , 9 , 10 ], T 4 AG 3 [ 12 ], A 2 TG 6 [ 13 ], T 2 AT 2 AG 3 [ 7 ], and T 6 AG 3 [ 14 , 15 ]—and at least seven variants, such as TCAG 2 [ 16 ], T 2 AG 2 C [ 17 ], T 2 CAG 2 [ 18 ], T 3 CAG 2 [ 18 ], C 3 TA 3 [ 19 ], T 2 N 4 AG 3 [...…”

Section: Discussionmentioning

confidence: 99%

“…ex A.Murr.) Koidz [ 41 ]. Furthermore, T 3 AG 3 has been observed at the interstitial regions and at all chromosome ends in Pinus spp.…”

Section: Introductionmentioning

confidence: 99%

FISH Mapping of Telomeric and Non-Telomeric (AG3T3)3 Reveal the Chromosome Numbers and Chromosome Rearrangements of 41 Woody Plants

Luo

Liu

et al. 2022

Genes

View full text Add to dashboard Cite

Data for the chromosomal FISH mapping localization of (AG3T3)3 are compiled for 37 species belonging 27 families; for 24 species and 14 families, this is the first such report. The chromosome number and length ranged from 14–136 and 0.56–14.48 μm, respectively. A total of 23 woody plants presented chromosome length less than 3 μm, thus belonging to the small chromosome group. Telomeric signals were observed at each chromosome terminus in 38 plants (90.5%) and were absent at several chromosome termini in only four woody plants (9.5%). Non-telomeric signals were observed in the chromosomes of 23 plants (54.8%); in particular, abundant non-telomeric (AG3T3)3 was obviously observed in Chimonanthus campanulatus. Telomeric signals outside of the chromosome were observed in 11 woody plants (26.2%). Overall, ten (AG3T3)3 signal pattern types were determined, indicating the complex genome architecture of the 37 considered species. The variation in signal pattern was likely due to chromosome deletion, duplication, inversion, and translocation. In addition, large primary constriction was observed in some species, probably due to or leading to chromosome breakage and the formation of new chromosomes. The presented results will guide further research focused on determining the chromosome number and disclosing chromosome rearrangements of woody plants.

show abstract

“…However, karyotype analysis is still a huge challenge in non-model plants. Although there are various available DNA probes for FISH in plants, such as repetitive sequences [19] and bacterial artificial chromosome (BAC) clones [20], etc., application in related species that diverged a few MYs ago has always shown an unsatisfactory result. For example, BAC probes are not suitable for FISH in some plant species with large complex genomes [21].…”

Section: Discussionmentioning

confidence: 99%

Chromosomal Characterization of Tripidium arundinaceum Revealed by Oligo-FISH

Fan

Chai

et al. 2021

IJMS

View full text Add to dashboard Cite

Sugarcane is of important economic value for producing sugar and bioethanol. Tripidium arundinaceum (old name: Erianthus arundinaceum) is an intergeneric wild species of sugarcane that has desirable resistance traits for improving sugarcane varieties. However, the scarcity of chromosome markers has hindered the cytogenetic study of T. arundinaceum. Here we applied maize chromosome painting probes (MCPs) to identify chromosomes in sorghum and T. arundinaceum using a repeated fluorescence in situ hybridization (FISH) system. Sequential FISH revealed that these MCPs can be used as reliable chromosome markers for T. arundinaceum, even though T. arundinaceum has diverged from maize over 18 MYs (million years). Using these MCPs, we identified T. arundinaceum chromosomes based on their sequence similarity compared to sorghum and labeled them 1 through 10. Then, the karyotype of T. arundinaceum was established by multiple oligo-FISH. Furthermore, FISH results revealed that 5S rDNA and 35S rDNA are localized on chromosomes 5 and 6, respectively, in T. arundinaceum. Altogether, these results represent an essential step for further cytogenetic research of T. arundinaceum in sugarcane breeding.

show abstract

Pre-labelled oligo probe-FISH karyotype analyses of four Araliaceae species using rDNA and telomeric repeat

Cited by 14 publications

References 75 publications

Oligo-FISH Can Identify Chromosomes and Distinguish Hippophaë rhamnoides L. Taxa

Oligo-FISH Can Identify Chromosomes and Distinguish Hippophaë rhamnoides L. Taxa

FISH Mapping of Telomeric and Non-Telomeric (AG3T3)3 Reveal the Chromosome Numbers and Chromosome Rearrangements of 41 Woody Plants

Chromosomal Characterization of Tripidium arundinaceum Revealed by Oligo-FISH

Contact Info

Product

Resources

About