Preclinical biological and physicochemical evaluation of two-photon engineered 3D biomimetic copolymer scaffolds for bone healing

Kampleitner, Carina; Changi, Katayoon; Felfel, Reda M.; Scotchford, Colin A.; Sottile, Virginie; Kluger, Robert; Hoffmann, Oskar; Grant, David M.; Epstein, Michelle M.

doi:10.1039/c9bm01827a

Cited by 12 publications

(17 citation statements)

References 58 publications

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“…Recently, to develop the bone repairing materials, one of the most important issues is bone homeostasis between osteoclasts and osteoblasts, because excessive differentiation of osteoclasts affects bone tissue resorption, which would occur metabolic bone-related diseases such as osteoporosis [ 32 , 33 , 34 ]. In general, when bone fracture occurs, both osteoblasts and osteoclasts are activated.…”

Section: Resultsmentioning

confidence: 99%

Promotion of Bone Regeneration Using Bioinspired PLGA/MH/ECM Scaffold Combined with Bioactive PDRN

et al. 2021

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Current approaches of biomaterials for the repair of critical-sized bone defects still require immense effort to overcome numerous obstacles. The biodegradable polymer-based scaffolds have been required to expand further function for bone tissue engineering. Poly(lactic-co-glycolic) acid (PLGA) is one of the most common biopolymers owing to its biodegradability for tissue regenerations. However, there are major clinical challenges that the byproducts of the PLGA cause an acidic environment of implanting site. The critical processes in bone repair are osteogenesis, angiogenesis, and inhibition of excessive osteoclastogenesis. In this study, the porous PLGA (P) scaffold was combined with magnesium hydroxide (MH, M) and bone-extracellular matrix (bECM, E) to improve anti-inflammatory ability and osteoconductivity. Additionally, the bioactive polydeoxyribonucleotide (PDRN, P) was additionally incorporated in the existing PME scaffold. The prepared PMEP scaffold has pro-osteogenic and pro-angiogenic effects and inhibition of osteoclast due to the PDRN, which interacts with the adenosine A2A receptor agonist that up-regulates expression of vascular endothelial growth factor (VEGF) and down-regulates inflammatory cytokines. The PMEP scaffold has superior biological properties for human bone-marrow mesenchymal stem cells (hBMSCs) adhesion, proliferation, and osteogenic differentiation in vitro. Moreover, the gene expressions related to osteogenesis and angiogenesis of hBMSCs increased and the inflammatory factors decreased on the PMEP scaffold. In conclusion, it provides a promising strategy and clinical potential candidate for bone tissue regeneration and repairing bone defects.

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Section: Resultsmentioning

confidence: 99%

Promotion of Bone Regeneration Using Bioinspired PLGA/MH/ECM Scaffold Combined with Bioactive PDRN

et al. 2021

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“…Several methacrylated or acrylated synthetic or biological polymers or hybrid materials are qualified, for instance hyaluronic acid, gelatin, polycaprolactone, or poly-lactide [14,[18][19][20][21]. Hybrid materials made of poly-lactide (PLA) and poly-caprolactone (PCL) performed well in previous studies [3,[22][23][24]. They combine hydrophilic, highly degradable PLA with slow degrading, hydrophobic PLC with excellent biocompatibility [14].…”

Section: Introductionmentioning

confidence: 86%

“…The present study is the first investigation of (i) the viability of human MSC as well as (ii) the differentiation of human PBMC into osteoclasts and (iii) the activity of osteoclasts in direct contact to porous scaffolds (PS) compared to compact scaffolds (CS) of LCM. In a previous study, Kampleitner et al (2020) analyzed the proliferation of murine MC3T3 osteoblast cell line and morphology of murine osteoclasts on porous scaffolds of LCM (pore size of 314 ± 14 µm) compared to pure cell culture plastic [3]. Thus, the major novelty of our study was the analysis of human cells on porous LCM scaffolds compared to compact scaffolds.…”

Section: Introductionmentioning

confidence: 97%

“…Progress in the field of 3D biomaterial generation allows the production of biphasic porous scaffolds that mimics the cartilage, tide zone, and subchondral bone. These biomimetic biphasic porous scaffolds should hold unlimited availability and personalized size adaption [3] capability [4]. Good biocompatibility would let them be the desired surgical option for the treatment of deep osteochondral defects.…”

Section: Introductionmentioning

confidence: 99%

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Porous 3D Scaffolds Enhance MSC Vitality and Reduce Osteoclast Activity

et al. 2021

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In the context of an aging population, unhealthy Western lifestyle, and the lack of an optimal surgical treatment, deep osteochondral defects pose a great challenge for the public health system. Biodegradable, biomimetic scaffolds seem to be a promising solution. In this study we investigated the biocompatibility of porous poly-((D,L)-lactide-ε-caprolactone)dimethacrylate (LCM) scaffolds in contrast to compact LCM scaffolds and blank cell culture plastic. Thus, morphology, cytotoxicity and metabolic activity of human mesenchymal stromal cells (MSC) seeded directly on the materials were analyzed after three and six days of culturing. Further, osteoclastogenesis and osteoclastic activity were assessed using reverse-transcriptase real-time PCR of osteoclast-specific genes, EIA and morphologic aspects after four, eight, and twelve days. LCM scaffolds did not display cytotoxic effects on MSC. After three days, metabolic activity of MSC was enhanced on 3D porous scaffolds (PS) compared to 2D compact scaffolds (CS). Osteoclast activity seemed to be reduced at PS compared to cell culture plastic at all time points, while no differences in osteoclastogenesis were detectable between the materials. These results indicate a good cytocompatibility of LCM scaffolds. Interestingly, porous 3D structure induced higher metabolic activity of MSC as well as reduced osteoclast activity.

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“…Since the first definition of the term “tissue engineering” in the late 1990s, biomaterial-based scaffolds have entered this domain in order to provide structural stability and suitable environment for cellular regeneration therefore “imitating” native tissue in functionality [ 5 ]. 3D scaffolds have been assessed for an extensive variety of applications ranging from bone [ 6 ], nerve [ 7 ], muscle, tendon/ligament [ 8 ] regeneration, and many more.…”

Section: Introductionmentioning

confidence: 99%

Scaffolds for Wound Healing Applications

2020

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In order to overcome the shortcomings related to unspecific and partially efficient conventional wound dressings, impressive efforts are oriented in the development and evaluation of new and effective platforms for wound healing applications. In situ formed wound dressings provide several advantages, including proper adaptability for wound bed microstructure and architecture, facile application, patient compliance and enhanced therapeutic effects. Natural or synthetic, composite or hybrid biomaterials represent suitable candidates for accelerated wound healing, by providing proper air and water vapor permeability, structure for macro- and microcirculation, support for cellular migration and proliferation, protection against microbial invasion and external contamination. Besides being the most promising choice for wound care applications, polymeric biomaterials (either from natural or synthetic sources) may exhibit intrinsic wound healing properties. Several nanotechnology-derived biomaterials proved great potential for wound healing applications, including micro- and nanoparticulate systems, fibrous scaffolds, and hydrogels. The present paper comprises the most recent data on modern and performant strategies for effective wound healing.

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Preclinical biological and physicochemical evaluation of two-photon engineered 3D biomimetic copolymer scaffolds for bone healing

Abstract: A major challenge in orthopedics is the repair of large non-union bone fractures.

Cited by 12 publications

References 58 publications

Promotion of Bone Regeneration Using Bioinspired PLGA/MH/ECM Scaffold Combined with Bioactive PDRN

Promotion of Bone Regeneration Using Bioinspired PLGA/MH/ECM Scaffold Combined with Bioactive PDRN

Porous 3D Scaffolds Enhance MSC Vitality and Reduce Osteoclast Activity

Scaffolds for Wound Healing Applications

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