In greenhouse energy balance models, the soil thermal parameters are important for evaluating the heat transfer between the greenhouse air and the soil. In this study, the soil thermal diffusivity was estimated from greenhouse soil temperature data using the amplitude, phase-shift, arctangent, logarithmic, and min-max methods. The results showed that the amplitude method and the min-max method performed well in estimating the soil thermal diffusivity. The obtained soil thermal diffusivity was input into a sinusoidal model to determine the greenhouse soil temperature at different soil depths. For greenhouse applications, the daily average soil temperature at different depths was predicted according to the temperature at the surface and the annual mean soil temperature. The model was validated using soil temperature data from summer and winter, when the greenhouse was cooled and heated, respectively.