Predictive autoimmunity using autoantibodies: screening for anti-nuclear antibodies

Pérez, Dolores; Gilburd, Boris; Cabrera-Marante, Óscar; Martínez-Flores, José Ángel; Serrano, Manuel; Naranjo, Laura; Pleguezuelo, Daniel; Morillas, Luis; Shovman, Ora; Paz‐Artal, Estela; Shoenfeld, Yehuda; Serrano, Antonio

doi:10.1515/cclm-2017-0241

Cited by 35 publications

(17 citation statements)

References 40 publications

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“…Among 110 subjects with negative ANA and positive ENA serology, 21% developed to positive ANA during the 2.5-year follow-up. This conversion rate is in agreement with the numbers reported in another study where it was ~25% for two years and ~76% for three years [13].…”

Section: Discussionsupporting

confidence: 93%

A Multiplex Autoantibody Panel for Early Detection of Autoimmune Disease Activity

Yang¹,

Krishna²,

Ranganathan³

et al. 2018

OJRA

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Background: Detection of autoantibodies has played a consolidate role in diagnosis of systemic autoimmune disorders. A cascade autoantibody testing is usually performed by employing antinuclear antibodies (ANA) test as a first screening test and the other tests as second level determinations. Here, we present that supplementing extractable nuclear antigens (ENA) tests to the ANA test may capture more autoimmunity and provide critical medical information at an early stage. In this study, we evaluated the clinical significance of a multiplex ANA + ENA panel. Methods: A cohort of 110 subjects, identified as ANA negative but ENA positive, were followed up for two years. The detection of their ANA and anti-ENA autoantibodies was assessed with a multiplex ANA + ENA panel at Vibrant America Clinical Laboratory. Results: During two years of multi-visit follow-up, 23 out of 110 subjects (20.9%) were found to become ANA positive within an average of 385 (±144) days. Histone (50/110, 45.5%) and Chromatin (25/110, 22.7%) antibodies were the most frequently found antibodies at their first visits. The subjects who were positive for RNP (5/8, 62.5%) and SSA (Ro) (10/22, 45.5%) have the highest ratio of conversion to positive ANA. No significant correlation was observed between the conversion frequency and the number of anti-ENA antibodies being carried. Conclusion: This study, which followed up on the subjects who had disparate ANA and ENA test results, showed that anti-ENA antibodies may exist years earlier than ANA. Combining ENA tests with ANA screening may reduce false negatives and improve sensitivity.

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Section: Discussionsupporting

confidence: 93%

A Multiplex Autoantibody Panel for Early Detection of Autoimmune Disease Activity

Yang¹,

Krishna²,

Ranganathan³

et al. 2018

OJRA

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“…For example, 22.5% of the anticellular antibody–negative SLE patients in our study still had anti‐SSA/Ro 60 antibodies using extractable nuclear antigen testing; 11.3% of our anticellular antibody–negative patients had anti‐dsDNA by chemiluminescence immunoassay. Our observations are consistent with a recent study showing that there is significant lack of agreement between positive results using a conventional multiplex array technology and the IIF on HEp‐2 cells .…”

Section: Discussionsupporting

confidence: 92%

“…Biomarkers such as autoantibodies and a variety of immune‐related and inflammation‐related molecules can appear years prior to clinical symptoms and/or the diagnosis of SLE and can accrue over time . Therefore, longitudinal studies are needed to evaluate the serologic status of anticellular antibody–negative and isolated CMP–positive patients over time and to evaluate whether the status varies with disease activity, damage accrual, therapeutic interventions, and/or specific substrate assays.…”

Section: Discussionmentioning

confidence: 99%

“…Therefore, longitudinal studies are needed to evaluate the serologic status of anticellular antibody–negative and isolated CMP–positive patients over time and to evaluate whether the status varies with disease activity, damage accrual, therapeutic interventions, and/or specific substrate assays. Even among ANA‐negative patients with lupus nephritis, the patient can take up to 10 years to seroconvert from ANA negative to positive . Some patients may only have detectable positive serologic results when there is uncontrolled disease activity due to loss of self‐tolerance from chronic autoreactivity of T and B cells .…”

Section: Discussionmentioning

confidence: 99%

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Antinuclear Antibody–Negative Systemic Lupus Erythematosus in an International Inception Cohort

Choi

Clarke

Pierre

et al. 2019

Arthritis Care & Research

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“…The most prevalent diagnosis was systemic lupus erythematous (37%) followed by Sjögren syndrome (23%). 20 Depending on the population you are looking at, the frequency numbers of WRDs are startling. For example, 1% to 4% of people who carry the gene for CD will manifest celiac.…”

mentioning

confidence: 99%

Food Sensitivities, Inflammation, and Autoimmune Disease: A Clinical Conversation with Tom O’Bryan, DC, CCN, DACBN, and Robert Rountree, MD

O'Bryan

Rountree²

2020

Alternative and Complementary Therapies

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Robert Rountree: I would like to start by hearing about how you got to where you are today. What was the trajectory of your educational and professional experience? You graduated from chiropractic school in 1981. That means it has been 38 years since you got that license. You have really come a long way since then! Tom O'Bryan: I went to the University of Michigan for undergrad. I think I was 19 at the time and I first read Prevention magazine, when it was a true health magazine, before it was sold to a pharmaceutical company and began advertising the benefits of drugs for different conditions. Jonathan Wright wrote case studies every month. I would read these case studies, and he had this very down-home, earthy kind of way of explaining medical conditions to patients in language they would understand and made sense to them. I did not realize until this interview that he was my first mentor on how to effectively communicate to patients. He would talk to this guy that had a condition, what were the symptoms and all that, and he recommended nutrition and diet and the guy would come back a few months later and he was much better. The references at the back of the article gave credibility to what he was talking about and it caught my interest. I said, "My gosh, there is really something to this holistic approach, that has science to validate it." I thought that was very interesting. The world that I was really in at the time, besides my studies, was the world of aikido. I had been lucky enough that the number-two guy in the world had come to Detroit to give a demonstration. He ultimately stayed and would have three to five classes per day, and a very special once a week by invitation only class called Kenshu, for a select group of the students. This man carried the direct unfiltered lineage of > 350 years of samurai in his family. These special weekly classes were on different concepts, one per week, of the samurai code of honor called Bushido. Dr. Rountree: So those were your formative yearsduring college, when you were studying aikido? Long before you went to chiropractic school? I would imagine that introduced you to a philosophy of how to approach health care. Dr. O'Bryan: That is exactly right. From there, I then went to Japan, and I lived in the world headquarters. I was a deshi. It was such an honor. On the surface it meant that I got to clean the toilets in the school, and whatever other menial tasks were assigned to me. I spent six months there and I was privileged to visit a number of the grand masters of different disciplines, jodo and kyodo and karate. I met some very famous grand masters. Dr. Rountree: Do you still do an aikido practice today? Dr. O'Bryan: Every day as a way of life. The whole premise of aikido is that you never fight force with force, it is that you deflect and allow the force to go in the direction it wants to go. That is what really attracted me. My girlfriend at the time, who became my wife later, was in a bad car accident when she was very young, and annually or so, she would have t...

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Predictive autoimmunity using autoantibodies: screening for anti-nuclear antibodies

Cited by 35 publications

References 40 publications

A Multiplex Autoantibody Panel for Early Detection of Autoimmune Disease Activity

A Multiplex Autoantibody Panel for Early Detection of Autoimmune Disease Activity

Antinuclear Antibody–Negative Systemic Lupus Erythematosus in an International Inception Cohort

Food Sensitivities, Inflammation, and Autoimmune Disease: A Clinical Conversation with Tom O’Bryan, DC, CCN, DACBN, and Robert Rountree, MD

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