Predominance of CTX-M-15 extended spectrum β-lactamases in diverse Escherichia coli and Klebsiella pneumoniae from hospital and community patients in Kuwait

Ensor, V. M.; Jamal, W.; Rotimi, Vincent O.; Evans, Jonathan D.; Hawkey, Peter M.

doi:10.1016/j.ijantimicag.2008.10.011

Cited by 37 publications

(36 citation statements)

References 8 publications

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“…Similarly, in most European countries, Latin America, and East Asia, CTX-M variants have displaced TEM and SHV enzymes as the predominant beta-lactamases produced by Gram-negative bacteria such as K. pneumoniae [3,27,32]. In Arab countries, the first description of CTX-M was in Egypt and then in the United Arab Emirates and Kuwait [39][40][41]. In these countries, the predominant ESBL was CTX-M-15.…”

Section: Discussionmentioning

confidence: 99%

Molecular characterization of extended-spectrum beta-lactamase producing Enterobacteriaceae in a Saudi Arabian tertiary hospital

Hassan

Abdalhamid

2014

J Infect Dev Ctries

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Introduction: The aim of this study was to determine the prevalence of extended-spectrum beta-lactamase (ESBL) producing Escherichia coli (E. coli), Klebsiella pneumoniae (K. pneumoniae), and Proteus mirabilis (P. mirabilis). In addition, different methods for detection of these enzymes, including the newly introduced CHROMagar ESBL, were evaluated. Methodology: A total of 382 Enterobacteriaceae clinical isolates were obtained from King Fahad Specialist Hospital -Dammam, during 2011 and screened for production of ESBL using advanced expert system of Vitek 2, CHROMagar and ESBL-E-strips. PCR assay was used to detect bla TEM , bla SHV , and bla CTX-M genes. Susceptibility to a panel of antibiotics was determined. Results: The overall proportion of ESBL-producing enterobacterial isolates was 30.6%, which was higher in E. coli (35.8%) than in K. pneumoniae (25.7%). ESBL genotypes showed remarkable increase in the CTX-M (97.4%) compared to SHV (23.1%). The predominant ESBL was CTX-M-15 (92.1 %). No TEM ESBL was detected in this study. The Vitek2 showed the highest sensitivity (100%), and the CHROMagar had the lowest specificity (97.3%) compared to the molecular method. All isolates were susceptible to imipenem and meropenem. Conclusions: This study confirms a high level of blaCTX-M positive ESBL isolates are circulating in the Eastern Province of Saudi Arabia. The trend of a multidrug-resistant profile associated with the recovery of the blaCTX-M gene is alarming.

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Section: Discussionmentioning

confidence: 99%

Molecular characterization of extended-spectrum beta-lactamase producing Enterobacteriaceae in a Saudi Arabian tertiary hospital

Hassan

Abdalhamid

2014

J Infect Dev Ctries

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“…isolates producing CTX-M-15-ESBL were obtained from non-Kuwaiti Arabs, while three of the CTX-M-15 producers were isolated from patients of Indian origin. Similarly, the majority of CTX-M-15-producing E. coli and K. pneumoniae isolates were found in patients from the Indian subcontinent and non-Kuwaiti Arabs with recent travel histories (76).…”

Section: Extended-spectrum and Ampc-type ␤-Lactamasesmentioning

confidence: 95%

“…Of 16 randomly selected ESBL-producing K. pneumoniae isolates and 27 E. coli isolates, CTX-M-15 was found in 10 out of 11 bla CTX-M -positive K. pneumoniae and 19 out of 21 bla CTX-M -positive E. coli strains isolated between 2005 and 2006. One E. coli isolate and another K. pneumoniae isolate produced CTX-M-9, while a single E. coli isolate was found to produce CTX-M-14 (76). An internationally prevalent E. coli clone (ST131) has been found to frequently produce CTX-M enzymes (78).…”

Section: Extended-spectrum and Ampc-type ␤-Lactamasesmentioning

confidence: 99%

“…Similarly, CTX-M-15-positive Enterobacteriaceae are also prevalent in Kuwait (54), UAE (52,54,56), and Oman (102-104) ( Table 2). This high prevalence may be due to immigrants arriving from countries with a high rate of CTX-M-15-positive Enterobacteriaceae, as typified in Kuwait, where the majority of CTX-M-15 producers were from patients from South Asia and non-Kuwaiti Arabs (76). About half of the population in Kuwait is from the Indian subcontinent and other Middle Eastern countries (116).…”

Section: Esbl and Carbapenemase Concerns In The Arabian Peninsulamentioning

confidence: 99%

“…It is well known that the ESBL type CTX-M-15 is ubiquitous in the Indian subcontinent (167)(168)(169)(170)(171). If inpatients are colonized with ESBL-or carbapenemases-positive bacteria, endogenous infections and/or patientto-patient transmission can occur, which may account for the high prevalence of CTX-M-15 producers isolated from hospitals in Gulf countries (52,54,56,76,84).…”

Section: Gram-negative Bacilli In the Arabian Peninsulamentioning

confidence: 99%

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β-Lactamase Production in Key Gram-Negative Pathogen Isolates from the Arabian Peninsula

et al. 2013

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SUMMARY Infections due to Gram-negative bacilli (GNB) are a leading cause of morbidity and mortality worldwide. The extent of antibiotic resistance in GNB in countries of the Gulf Cooperation Council (GCC), namely, Saudi Arabia, United Arab Emirates, Kuwait, Qatar, Oman, and Bahrain, has not been previously reviewed. These countries share a high prevalence of extended-spectrum-β-lactamase (ESBL)- and carbapenemase-producing GNB, most of which are associated with nosocomial infections. Well-known and widespread β-lactamases genes (such as those for CTX-M-15, OXA-48, and NDM-1) have found their way into isolates from the GCC states. However, less common and unique enzymes have also been identified. These include PER-7, GES-11, and PME-1. Several potential risk factors unique to the GCC states may have contributed to the emergence and spread of β-lactamases, including the unnecessary use of antibiotics and the large population of migrant workers, particularly from the Indian subcontinent. It is clear that active surveillance of antimicrobial resistance in the GCC states is urgently needed to address regional interventions that can contain the antimicrobial resistance issue.

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Evolution of β-Lactamases: Past, Present, and Future

Bush

2011

Antibiotic Discovery and Development

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Predominance of CTX-M-15 extended spectrum β-lactamases in diverse Escherichia coli and Klebsiella pneumoniae from hospital and community patients in Kuwait

Cited by 37 publications

References 8 publications

Molecular characterization of extended-spectrum beta-lactamase producing Enterobacteriaceae in a Saudi Arabian tertiary hospital

Molecular characterization of extended-spectrum beta-lactamase producing Enterobacteriaceae in a Saudi Arabian tertiary hospital

β-Lactamase Production in Key Gram-Negative Pathogen Isolates from the Arabian Peninsula

Evolution of β-Lactamases: Past, Present, and Future

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