Preferential glial cell attachment to microcontact printed surfaces

John, Pamela M. St.; Kam, Lance C.; Turner, Stephen W.; Craighead, Harold G.; Issacson, M.; Turner, James N.; Shain, William

doi:10.1016/s0165-0270(97)00069-1

Cited by 75 publications

(38 citation statements)

References 13 publications

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“…This assertion that methyl-terminated, alkyl surfaces can be excellent substrates for human cell culture is unique among the published reports of cellular interactions with alkyl monolayers. 27,29,[31][32][33]35,36 Our findings are not surprising, however, because as motile phagocytes, macrophages must be able to adhere to a wide range of foreign materials and reside in a variety of human tissues.…”

Section: Discussionmentioning

confidence: 68%

“…26 Short and long chain alkyl-modified surfaces were both used extensively as model surfaces for studying biological interactions with proteins, bacteria, and cells. [27][28][29][30][31][32][33][34][35][36] These in vitro studies demonstrated that alkyl-modified surfaces make poor cellular substrates for most cell types including osteoblasts, neurons, fibroblasts, endothelial cells, and glial cells. The primary aim of this study was to determine if the reports of reduced cellular adhesion on long chain alkyl surfaces are applicable to human monocytes and monocyte derived macrophages, which we previously showed to strongly adhere to a diverse range of surface chemistries.…”

Section: Introductionmentioning

confidence: 97%

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Alkylsilane-modified surfaces: Inhibition of human macrophage adhesion and foreign body giant cell formation

Jenney

Anderson

1999

J. Biomed. Mater. Res.

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A homologous set of alkylsilane-modified glass surfaces with chain lengths ranging from methyl to octadecyl was prepared in order to examine the influence of alkyl surface chemistry on macrophage adhesion and foreign body giant cell (FBGC) formation. Contact angle and X-ray photoelectron spectroscopy analysis confirmed our silanation technique and indicated a consistent alkyl chain density independent of chain length. Human peripheral blood monocytes were isolated and cultured on these alkylsilane surfaces for a period of 10 days. The initial density of human monocytes was similar on all surfaces. Beyond day 0 the clean glass, methyl (DM and C1), propyl (C3), and hexyl (C6) surfaces maintained a high cell density and supported macrophage development. In contrast, long-term macrophage density was extremely low on the tetradecyl (C14) and octadecyl (C18) surfaces. When interleukin-4 was added to induce FBGC formation in vitro, the DM, C1, C3, and C6 surfaces supported high levels of macrophage fusion while clean glass strongly inhibited fusion. The C14 and C18 surfaces did not contain sufficient macrophages to support FBGC formation. Cage implant studies revealed that in vivo macrophage density and FBGC formation on clean glass and C6 surfaces was similar to in vitro data. In contrast to the monocyte culture results, the C18 cage implant samples supported significant FBGC formation, possibly as a result of different conditions within each experimental system. Radiotracer adsorption studies of eight human serum proteins identified the high concentration and tenacious hold of adsorbed von Willebrand factor as being possibly involved in the poor long-term macrophage density observed on C14 and C18.

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Section: Discussionmentioning

confidence: 68%

Section: Introductionmentioning

confidence: 97%

Alkylsilane-modified surfaces: Inhibition of human macrophage adhesion and foreign body giant cell formation

Jenney

Anderson

1999

J. Biomed. Mater. Res.

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“…Hydrophobic alkylsilanes may indirectly constitute another cell-repellent surface. St. John and coworkers 205,206 studied the attachment and growth of astrocytes on silicon surfaces microstamped with patterns of DETA (hydrophilic and cell adhesive) surrounded by octadecyltrichlorosilane (OTS, hydrophobic, and cell repellent). LRM55 cells (a rat astrocyte cell line) attached selectively to DETA SAM areas with minimal attachment to OTS areas.…”

Section: Vc Glial Cell Biology On a Chipmentioning

confidence: 99%

Biology on a Chip: Microfabrication for Studying the Behavior of Cultured Cells

Tourovskaia

Folch

2003

Crit Rev Biomed Eng

173

140

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The ability to culture cells in vitro has revolutionized hypothesis testing in basic cell and molecular biology research and has become a standard methodology in drug screening and toxicology assays. However, the traditional cell culture methodology-consisting essentially of the immersion of a large population of cells in a homogeneous fluid medium-has become increasingly limiting, both from a fundamental point of view (cells in vivo are surrounded by complex spatiotemporal microenvironments) and from a practical perspective (scaling up the number of fluid handling steps and cell manipulations for high-throughput studies in vitro is prohibitively expensive). Micro fabrication technologies have enabled researchers to design, with micrometer control, the biochemical composition and topology of the substrate, the medium composition, as well as the type of neighboring cells surrounding the microenvironment of the cell. In addition, microtechnology is conceptually well suited for the development of fast, low-cost in vitro systems that allow for high-throughput culturing and analysis of cells under large numbers of conditions. Here we review a variety of applications of microfabrication in cell culture studies, with an emphasis on the biology of various cell types.

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“…[14][15][16] Usually, selective cell attachment is achieved indirectly by microfabricating a template to which cells adhere preferentially. The template may be made of metals, 1,2 self-assembled monolayers, 3,4,6,7,11,17 polymers, 5,8 extracellular matrix proteins, [13][14][15][16] celladhesive peptides, 9,10,12 or a combination thereof. This strategy has several drawbacks: (1) by the very presence of the template, cellular micropatterns on homogeneous surfaces are not possible; (2) for a given template, attachment selectivity varies broadly with cell type; (3) the composition of the template may be affected by proteins adsorbed from the seeding medium before the cells actually "see" the surface; and (4) in many cases, fabrication of the template requires experimental methods such as surface chemical modification, biochemical synthesis, and/or microfabrication, which are not readily available in most biological laboratories.…”

Section: Introductionmentioning

confidence: 99%

Microfabricated elastomeric stencils for micropatterning cell cultures

Folch

Hurtado

et al. 2000

J. Biomed. Mater. Res.

329

250

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Here we present an inexpensive method to fabricate microscopic cellular cultures, which does not require any surface modification of the substrate prior to cell seeding. The method utilizes a reusable elastomeric stencil (i.e., a membrane containing thru holes) which seals spontaneously against the surface. The stencil is applied to the cell-culture substrate before seeding. During seeding, the stencil prevents the substrate from being exposed to the cell suspension except on the hole areas. After cells are allowed to attach and the stencil is peeled off, cellular islands with a shape similar to the holes remain on the cell-culture substrate. This solvent-free method can be combined with a wide range of substrates (including biocompatible polymers, homogeneous or nonplanar surfaces, microelectronic chips, and gels), biomolecules, and virtually any adherent cell type.

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Preferential glial cell attachment to microcontact printed surfaces

Cited by 75 publications

References 13 publications

Alkylsilane-modified surfaces: Inhibition of human macrophage adhesion and foreign body giant cell formation

Alkylsilane-modified surfaces: Inhibition of human macrophage adhesion and foreign body giant cell formation

Biology on a Chip: Microfabrication for Studying the Behavior of Cultured Cells

Microfabricated elastomeric stencils for micropatterning cell cultures

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