Preferential immobilization of biomolecules on silicon microstructure array by means of electron beam lithography on organosilane self-assembled monolayer resist

Takenaka, Takashi; Hosaka, Takumi; Miyake, Takeo; Zhang, Guo Jun; Zako, Tamotsu; Funatsu, Takashi; Ohdomari, Iwao

doi:10.1016/j.apsusc.2004.05.033

Cited by 26 publications

(15 citation statements)

References 10 publications

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“…Various protocols describing the immobilization of oligonucleotides onto surfaces can be found in the literature 23–24, 32–33. Unfortunately, lack of experimental details or characterization aspects prevented us from preparing suitable surfaces for our purposes based on these studies.…”

Section: Resultssupporting

confidence: 91%

Oligonucleotide Nanostructured Surfaces: Effect on Escherichia coli Curli Expression

Cottenye

Teixeira

Ponche

et al. 2008

Macromolecular Bioscience

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Oligonucleotide model surfaces allowing independent variation of topography and chemical composition were designed to study the adhesion and biofilm growth of E.coli. Surfaces were produced by covalent binding of oligonucleotides and immobilization of nucleotide-based vesicles. Their properties were confirmed through a combination of fluorescence microscopy, XPS, ellipsometry, AFM and wettability studies at each step of the process. These surfaces were then used to study the response of three different strains of E.coli quantified in a static biofilm growth mode. This study led to convincing evidence that oligonucleotide-modified surfaces, independent of the topographical feature used in this study, enhanced curli expression without an increase in the number of adherent bacteria.

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Section: Resultssupporting

confidence: 91%

Oligonucleotide Nanostructured Surfaces: Effect on Escherichia coli Curli Expression

Cottenye

Teixeira

Ponche

et al. 2008

Macromolecular Bioscience

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“…Unlike the process described in Ref. [13], the lift-off is performed after the glutaraldehyde grafting because the molecule is very reactive and will be physisorbed on the substrate if the resist is removed before. The lift-off is facilitated by the large difference between the thickness of the PMMA layer and the APTES one, 270 nm against 1 nm.…”

Section: Functionalization Processmentioning

confidence: 99%

Bio-Functionalization of Silicon Carbide Nanostructures for SiC Nanowire-Based Sensors Realization

Fradetal¹,

Stambouli²,

Bano³

et al. 2014

j. nanosci. nanotech.

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The bio-functionalization process consisting in grafting desoxyribo nucleic acid via aminopropyl-triethoxysilane is performed on several kinds of silicon carbide nanostructures. Prior, the organic layer is characterized on planar surface with fluorescence microscopy and X-ray photoelectron spectroscopy. Then, the functionalization is performed on two kinds of nanopillar arrays. One is composed of top-down SiC nanopillars with a wide pitch of 5 microm while the other one is a dense array (pitch: 200 nm) of core-shell Si-SiC nanowires obtained by carburization of silicon nanowires. Depending on both the pillar morphology and the pitch, different results in term of DNA surface coverages are obtained, as seen from fluorescence microscopy images. Particularly, in the case of the wide pitch array, it has been shown that the DNA molecules are located all along the nanopillars. To achieve a DNA sensor based on a nanowire-field effect transistor, the functionalization must be conducted on a single SiC nanowire or nanopillar that constitutes the channel of the field effect transistor. The localization of the functionalization in a small area around the nanostructures guarantees high performances to the sensor. In this aim, the functionalization process is combined with common microelectronics techniques of lithography and lift-off. The DNA immobilization is investigated by fluorescence microscopy and atomic force microscopy.

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“…[50] It is also possible to chemically modify SAMs in a specific manner by electrons. [33,[52][53][54][55][56][57][58][59] …”

Section: Nanoscaled Lateral Structurization Of Sams For Surface Functmentioning

confidence: 99%

Interfacial Systems Chemistry: Out of the Vacuum—Through the Liquid—Into the Cell

Wöll

2010

ChemPhysChem

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Preferential immobilization of biomolecules on silicon microstructure array by means of electron beam lithography on organosilane self-assembled monolayer resist

Cited by 26 publications

References 10 publications

Oligonucleotide Nanostructured Surfaces: Effect on Escherichia coli Curli Expression

Oligonucleotide Nanostructured Surfaces: Effect on Escherichia coli Curli Expression

Bio-Functionalization of Silicon Carbide Nanostructures for SiC Nanowire-Based Sensors Realization

Interfacial Systems Chemistry: Out of the Vacuum—Through the Liquid—Into the Cell

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