Pregnancy and birth resulting from transfer of a blastocyst observed to have one pronucleus at the time of examination for fertilization: Case report

Gras, Lyn; Trounson, Alan

doi:10.1093/humrep/14.7.1869

Cited by 37 publications

(29 citation statements)

References 11 publications

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“…Gras and Trounson suggested extended culture beyond the cleavage stage in an effort to assess the potential of these embryos to develop to the blastocyst stage as a better predictor of genetic normalcy (1). In the present report, our patient was 41 years old and had a markedly elevated FSH level.…”

Section: Discussionmentioning

confidence: 56%

“…Staessen and Van Steirteghem found that 48.7% of embryos developing from single pronucleated oocytes were diploid after IVF and 27.9% after ICSI (7). The potential of embryos derived from such oocytes to cause a normal pregnancy has been the subject of only three reports in the literature (1,6,8). The initial report by Staessen et al described two births out of 38 transfers of a single embryo resulting from a zygote with one pronucleus (6).…”

Section: Discussionmentioning

confidence: 99%

“…Single pronuclei are sometimes observed and cause a concern in the IVF laboratory as these may be the result of parthenogenetic activation, failure of either gamete to progress with pronucleus formation, disynchronous appearance of pronuclei, or male and female pronuclear fusion. Although some of these oocytes may actually be normally fertilized and diploid, due to the uncer-tainty as to their genetic normalcy, they are often discarded (1).…”

Section: Introductionmentioning

confidence: 99%

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Monozygotic Twin Birth After the Transfer of a Cleavage Stage Embryo Resulting from a Single Pronucleated Oocyte

Dasig

Lyon

Behr

et al. 2004

J Assist Reprod Genet

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Purpose : To present a case involving the transfer of a single pronucleated oocyte resulting in a monozygotic twin pregnancy. Method : A descriptive case report of a single patient. Results : The patient conceived and was found to have a monochorionic diamnionic pregnancy which resulted in the birth of normal identical twin boys at 32 weeks of gestation. Conclusions : The case report addresses an issue that has not received proper attention in the literature. It illustrates that observing a single PN in an oocyte at fertilization check should not be an absolute deterrent to transferring the resulting embryo even in an older patient with a high FSH level. This report also suggests that single observations, especially at the assessment of fertilization, in the IVF laboratory are limited when evaluating embryo potential and normalcy.

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Section: Discussionmentioning

confidence: 56%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Monozygotic Twin Birth After the Transfer of a Cleavage Stage Embryo Resulting from a Single Pronucleated Oocyte

Dasig

Lyon

Behr

et al. 2004

J Assist Reprod Genet

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“…On the one hand, cytogenetic analysis has revealed that a proportion of diploid embryos can develop from 1PN zygotes [1]. Healthy babies developing from such embryos have been reported [2]. On the other hand, 2PN zygotes are not necessarily euploid.…”

Section: Introductionmentioning

confidence: 99%

Live births resulting from 0PN-derived embryos in conventional IVF cycles

Liu

Wang

Zhang³

et al. 2016

J Assist Reprod Genet

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Purpose The aim of this study was to (1) investigate the incidence of embryos derived from Bunfertilized oocytes^i.e., oocytes not displaying pronuclei (0PN) at the time of the fertilization check and (2) determine the clinical pregnancy rates when transferring 0PN-derived embryos. Methods In this retrospective study, 4424 IVF-ET cycles were reviewed. Results In total, 11.3 % (4966/43,949) 0PN-derived embryos were observed. It was found that female age, number of oocytes, and the top-quality embryo rate were significantly correlated with 0PN-derived embryo occurrence. The source of embryos transferred did not impact significantly on clinical pregnancy and livebirth rates. Of the 183 cycles included in this study where 275 0PN-derived embryos were transferred in total, only 0PN-derived embryos were available in 70 of those cycles. It was noteworthy that 13 healthy infants resulted from 0PN-derived embryos with an implantation rate of 17.0 %. Conclusion These results indicate that the traditional method of excluding embryos because of those oocytes originally lacking any sign of a pronucleus at the fertilization check should be reconsidered as transferring 0PN-derived embryos with subsequent expected developmental performance may be considered as an option for those patients where no other embryos are available.Keywords Abnormal fertilization . Pronucleus . 0PN . Pregnancy . ImplantationIn in vitro fertilization (IVF) or intracytoplasmic sperm injection (ICSI) treatment cycles, a fertilization check is performed at 17-20 h post insemination to exclude oocytes that have fertilized abnormally or not fertilized at all. Normal fertilization of an oocyte is defined by observing two distinct pronuclei (2PN) and two polar bodies after insemination. Oocytes showing no pronucleus (0PN), one pronucleus (1PN), or three pronuclei (3PN) (or more) are deemed as having fertilized abnormally and may be discarded. However, this assumption may not always be accurate. On the one hand, cytogenetic analysis has revealed that a proportion of diploid embryos can develop from 1PN zygotes [1]. Healthy babies developing from such embryos have been reported [2]. On the other hand, 2PN zygotes are not necessarily euploid. Furthermore, there are reports of 0PN-derived embryos present in 12 to 20 % of day 3 embryos [3][4][5] which result in healthy babies [4,6,7]. Although to transfer such embryos in the absence of the signs of normal fertilization may have become an option in clinical policy, it is still a dilemma for the clinical team when it comes to carrying this out in practice.In this study, fertilization results from conventional IVF were reviewed to (1) investigate the incidence of embryos derived from 0PN oocytes and (2) determine the clinical pregnancy rates (CPR) when transferring such 0PN embryos.

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“…Although the majority of 1PN zygotes are incapable of further development, a small number develop to the blastocyst stage. Moreover, they successfully progress to a live birth in cases where no other embryos exist of better quality [30][31][32] and despite the low pregnancy rate. Nevertheless, because of the uncertainty of their genetic normality, transferring such embryos is controversial among ART laboratories.…”

Section: Aberrant Behavior Of Female Pn (Fpn) During the Pronuclear Smentioning

confidence: 99%

Observation of human embryonic behavior in vitro by high‐resolution time‐lapse cinematography

Iwata

Mio²

2016

Reprod Medicine & Biology

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Assisted reproductive technology (ART) has yielded vast amounts of information and knowledge on human embryonic development in vitro; however, still images provide limited data on dynamic changes in the developing embryos. Using our high-resolution time-lapse cinematography (hR-TLC) system, we were able to describe normal human embryonic development continuously from the fertilization process to the hatched blastocyst stage in detail. Our hR-TLC observation also showed the embryonic abnormality of a third polar body (PB)-like substance likely containing a small pronucleus being extruded and resulting in single-pronucleus (1PN) formation, while our molecular biological investigations suggested the possibility that some 1PN embryos could be diploid, carrying both maternal and paternal genomes. Furthermore, in some embryos the extruded third PB-like substance was eventually re-absorbed into the ooplasm resulting in the formation of an uneven-sized, two-PN zygote. In addition, other hR-TLC observations showed that cytokinetic failure was correlated with equal-sized, multi-nucleated blastomeres that were also observed in the embryo showing early initiation of compaction. Assessment combining our hR-TLC with molecular biological techniques enables a better understanding of embryonic development and potential improvements in ART outcomes.

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Pregnancy and birth resulting from transfer of a blastocyst observed to have one pronucleus at the time of examination for fertilization: Case report

Cited by 37 publications

References 11 publications

Monozygotic Twin Birth After the Transfer of a Cleavage Stage Embryo Resulting from a Single Pronucleated Oocyte

Monozygotic Twin Birth After the Transfer of a Cleavage Stage Embryo Resulting from a Single Pronucleated Oocyte

Live births resulting from 0PN-derived embryos in conventional IVF cycles

Observation of human embryonic behavior in vitro by high‐resolution time‐lapse cinematography

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