Preliminary field test of lyophilised contagious caprine pleuropneumonia vaccine

Rurangirwa, F. R.; McGuire, T C; Mbai, L.; Ndung'u, Loise; Wambugu, A.

doi:10.1016/0034-5288(91)90114-4

Cited by 21 publications

(17 citation statements)

References 6 publications

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“…These features are collectively consistent with chronic fibrinous bronchopneumonia [8,15] and thus compatible with poor resolution of pneumonia from M. capripneumoniae infection. However, the agent was not grown from the lungs, in accordance with previous experiences from attempts to cultivate M. capripneumoniae from pulmonary or pleural lesions late in experimental infection [18,28,35]. In our study, cultivation was negative despite use of pyruvate-containing media known to markedly support growth [4,22].…”

Section: Discussionsupporting

confidence: 87%

“…Long term survivors of acute disease may display chronic pleuropneumonia or chronic pleuritis [13,28] but cultural recovery of the agent has not been demonstrated in such late stage pulmonary lesions [18,35]. Still, negative results of cultivation of M. capripneumoniae is not proof of freedom of infection [32] and the use of complementary techniques for microbial identification is indicated.…”

Section: Introductionmentioning

confidence: 99%

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Experimental Contagious Caprine Pleuropneumonia: A Long Term Study on the Course of Infection and Pathology in a Flock of Goats Infected with Mycoplasma capricolum subsp. capripneumoniae

et al. 2004

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: Experimental contagious caprine pleuropneumonia: A long term study on the course of infection and pathology in a flock of goats infected with Mycoplasma capricolum subsp. capripneumoniae. Acta vet. scand. 2004, 45, 167-179. -Contagious caprine pleuropneumonia (CCPP) is a major threat to goat farming in parts of Africa and Asia. It classically causes acute high morbidity and mortality early in infection, but little is known of its long term epizootiology and course. In this study, 10 goats were inoculated with Mycoplasma capricolum subsp. capripneumoniae (M. capripneumoniae) and then mixed with 15 goats for contact transmission. The disease course was monitored in each goat for 56-105 days, whereafter the goats were killed and necropsied. Varying features signifying infection occurred in altogether 17 goats (7 inoculated, 10 in-contact). Clinical signs were severe in 8 goats but no fatalities occurred. Only 6 goats had serum antibody titres against M. capripneumoniae in ELISA. Fourteen goats (5 inoculated, 9 in-contact) had chronic pleuropulmonary lesions compatible with CCPP at necropsy and 7 of those showed M. capripneumoniae antigen in the lung by immunohistochemistry. Neither cultivation nor PCR tests were positive for the agent in any goat. The results indicate that the clinical course of CCPP in a flock may be comparatively mild, M. capripneumoniaeassociated lung lesions may be present at a late stage of infection, and chronic infection may occur without a significant serological response. goat; Mycoplasma; contagious pleuropneumonia; ELISA; immunohistochemistry; serology; pathology.

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Section: Discussionsupporting

confidence: 87%

Section: Introductionmentioning

confidence: 99%

Experimental Contagious Caprine Pleuropneumonia: A Long Term Study on the Course of Infection and Pathology in a Flock of Goats Infected with Mycoplasma capricolum subsp. capripneumoniae

et al. 2004

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“…Such trials would also be useful to determine the true sensitivity of the test, which is difficult to evaluate with serum samples collected in the field. Quality control is particularly important for vaccines, as the industrial product used is generated on a large scale and may behave differently from the original laboratory product for which efficacy was demonstrated [29,30]. The cELISA test could be used, in particular, to evaluate the stability of the vaccine in liquid form after storage at +4°C.…”

Section: Discussionmentioning

confidence: 99%

An international collaborative study to determine the prevalence of contagious caprine pleuropneumonia by monoclonal antibody-based cELISA

et al. 2014

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BackgroundFew serological tests are available for detecting antibodies against Mycoplasma capricolum subsp. capripneumoniae, the causal agent of contagious caprine pleuropneumonia (CCPP). The complement fixation test, the test prescribed for international trade purposes, uses a crude antigen that cross-reacts with all the other mycoplasma species of the “mycoides cluster” frequently infecting goat herds. The lack of a more specific test has been a real obstacle to the evaluation of the prevalence and economic impact of CCPP worldwide. A new competitive ELISA kit for CCPP, based on a previous blocking ELISA, was formatted at CIRAD and used to evaluate the prevalence of CCPP in some regions of Kenya, Ethiopia, Mauritius, Tajikistan and Pakistan in an international collaborative study.ResultsThe strict specificity of the test was confirmed in CCPP-free goat herds exposed to other mycoplasma species of the “mycoides cluster”. Prevalence studies were performed across the enzootic range of the disease in Africa and Asia. Seroprevalence was estimated at 14.6% in the Afar region of Ethiopia, whereas all the herds presented for CCPP vaccination in Kenya tested positive (individual seroprevalence varied from 6 to 90% within each herd). In Mauritius, where CCPP emerged in 2009, nine of 62 herds tested positive. In Central Asia, where the disease was confirmed only recently, no positive animals were detected in the Wakhan District of Afghanistan or across the border in neighboring areas of Tajikistan, whereas seroprevalence varied between 2.7% and 44.2% in the other districts investigated and in northern Pakistan. The test was also used to monitor seroconversion in vaccinated animals.ConclusionsThis newly formatted CCPP cELISA kit has retained the high specificity of the original kit. It can therefore be used to evaluate the prevalence of CCPP in countries or regions without vaccination programs. It could also be used to monitor the efficacy of vaccination campaigns as high-quality vaccines induce high rates of seroconversion.

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“…15, 16 Although some animals had very high titers, no clinical signs were observed and mycoplasma cultures were negative. 18 The possible occurrence of CCPP in wild herbivores in contact with livestock has been investigated in Kenya. 17 Isolation of mycoplasmas other than Mccp has been reported in other wild species such as the Rocky mountain bighorn sheep (Ovis Canadensis), mouflon (Ovis musimon), aoudad (Ammotragus lervi), 14,23 and Vaal rhebok (Pelea capreolus).…”

Section: Introductionmentioning

confidence: 99%

Contagious Caprine Pleuropneumonia Outbreak in Captive Wild Ungulates at Al Wabra Wildlife Preservation, State of Qatar

Arif¹,

Schulz²,

Thiaucourt³

et al. 2007

Journal of Zoo and Wildlife Medicine

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Contagious caprine pleuropneumonia (CCPP) caused by Mycoplasma capricolum subsp. capripneumoniae is a highly contagious and serious respiratory disease of domestic goats, characterized by coughing, severe respiratory distress, and high mortality rates. The lesions at necropsy are mainly a fibrinous pleuropneumonia with increased straw-colored pleural fluid. An outbreak of CCPP in wild goat (Capra aegagrus), Nubian ibex (Capra ibex nubiana), Laristan mouflon (Ovis orientalis laristanica), and gerenuk (Litocranius walleri) occurred at Al Wabra Wildlife Preservation in the State of Qatar. The disease was suspected because of the clinical symptoms and the necropsy findings and was confirmed by the isolation and identification of the causative organism. This new finding indicates that CCPP should be considered a potential threat to wildlife and the conservation of endangered ruminant species, especially in the Middle East, where it is enzootic because of its presence in chronic carriers. Susceptible imported animals should be quarantined and vaccinated. The preferred samples for diagnosis are the pleural fluid, which contains high numbers of Mycoplasma, and sections of hepatized lung, preferably at the interface of normal and diseased tissues. Samples must be shipped to diagnostic laboratories rapidly, and appropriate cool conditions must be maintained during shipping.

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Preliminary field test of lyophilised contagious caprine pleuropneumonia vaccine

Cited by 21 publications

References 6 publications

Experimental Contagious Caprine Pleuropneumonia: A Long Term Study on the Course of Infection and Pathology in a Flock of Goats Infected with Mycoplasma capricolum subsp. capripneumoniae

Experimental Contagious Caprine Pleuropneumonia: A Long Term Study on the Course of Infection and Pathology in a Flock of Goats Infected with Mycoplasma capricolum subsp. capripneumoniae

An international collaborative study to determine the prevalence of contagious caprine pleuropneumonia by monoclonal antibody-based cELISA

Contagious Caprine Pleuropneumonia Outbreak in Captive Wild Ungulates at Al Wabra Wildlife Preservation, State of Qatar

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